Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer
Summary: Mitochondrial metabolism is a critical mechanism that is deregulated in numerous retinal diseases. Here, we elaborate a protocol to quantify oxygen consumption rate as a measure of mitochondrial respiration directly from mouse retinal tissue pieces. Our procedure combines the use of Seahors...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721002409 |
| _version_ | 1831757552574005248 |
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| author | Trupti Shetty Bomina Park Timothy W. Corson |
| author_facet | Trupti Shetty Bomina Park Timothy W. Corson |
| author_sort | Trupti Shetty |
| collection | DOAJ |
| description | Summary: Mitochondrial metabolism is a critical mechanism that is deregulated in numerous retinal diseases. Here, we elaborate a protocol to quantify oxygen consumption rate as a measure of mitochondrial respiration directly from mouse retinal tissue pieces. Our procedure combines the use of Seahorse extracellular flux technology and ex vivo retinal tissue isolation and is robustly reproducible under different treatment conditions. This protocol allows direct assessment of mitochondrial function in response to drug treatments or genetic manipulation in mouse models.For complete details on the use and execution of this protocol, please refer to Shetty et al. (2020), Sardar Pasha et al. (2021), Kooragayala et al. (2015), and Joyal et al. (2016). |
| first_indexed | 2024-12-22T00:33:13Z |
| format | Article |
| id | doaj.art-7db06dd5cc054caf93be94225e64f0e8 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T00:33:13Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-7db06dd5cc054caf93be94225e64f0e82022-12-21T18:44:53ZengElsevierSTAR Protocols2666-16672021-06-0122100533Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzerTrupti Shetty0Bomina Park1Timothy W. Corson2Eugene and Marilyn Glick Eye Institute, Department of Ophthalmology, Indiana University School of Medicine, Indianapolis, IN 46202, USA; Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, IN 46202, USA; Corresponding authorEugene and Marilyn Glick Eye Institute, Department of Ophthalmology, Indiana University School of Medicine, Indianapolis, IN 46202, USA; Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, IN 46202, USAEugene and Marilyn Glick Eye Institute, Department of Ophthalmology, Indiana University School of Medicine, Indianapolis, IN 46202, USA; Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, IN 46202, USA; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA; Corresponding authorSummary: Mitochondrial metabolism is a critical mechanism that is deregulated in numerous retinal diseases. Here, we elaborate a protocol to quantify oxygen consumption rate as a measure of mitochondrial respiration directly from mouse retinal tissue pieces. Our procedure combines the use of Seahorse extracellular flux technology and ex vivo retinal tissue isolation and is robustly reproducible under different treatment conditions. This protocol allows direct assessment of mitochondrial function in response to drug treatments or genetic manipulation in mouse models.For complete details on the use and execution of this protocol, please refer to Shetty et al. (2020), Sardar Pasha et al. (2021), Kooragayala et al. (2015), and Joyal et al. (2016).http://www.sciencedirect.com/science/article/pii/S2666166721002409MetabolismModel OrganismsNeuroscience |
| spellingShingle | Trupti Shetty Bomina Park Timothy W. Corson Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer STAR Protocols Metabolism Model Organisms Neuroscience |
| title | Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer |
| title_full | Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer |
| title_fullStr | Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer |
| title_full_unstemmed | Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer |
| title_short | Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer |
| title_sort | measurement of mitochondrial respiration in the murine retina using a seahorse extracellular flux analyzer |
| topic | Metabolism Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721002409 |
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