Electric field bridging-effect in electrified microfibrils’ scaffolds
Introduction: The use of biocompatible scaffolds combined with the implantation of neural stem cells, is increasingly being investigated to promote the regeneration of damaged neural tissue, for instance, after a Spinal Cord Injury (SCI). In particular, aligned Polylactic Acid (PLA) microfibrils’ sc...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1264406/full |
| _version_ | 1797649438832852992 |
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| author | Sara Fontana Sara Fontana Laura Caramazza Laura Caramazza Paolo Marracino Irene Cuenca Ortolá Micol Colella Noemi Dolciotti Alessandra Paffi Fernando Gisbert Roca Sergiy Ivashchenko Jorge Más Estellés Claudia Consales Marco Balucani Francesca Apollonio Francesca Apollonio Micaela Liberti Micaela Liberti |
| author_facet | Sara Fontana Sara Fontana Laura Caramazza Laura Caramazza Paolo Marracino Irene Cuenca Ortolá Micol Colella Noemi Dolciotti Alessandra Paffi Fernando Gisbert Roca Sergiy Ivashchenko Jorge Más Estellés Claudia Consales Marco Balucani Francesca Apollonio Francesca Apollonio Micaela Liberti Micaela Liberti |
| author_sort | Sara Fontana |
| collection | DOAJ |
| description | Introduction: The use of biocompatible scaffolds combined with the implantation of neural stem cells, is increasingly being investigated to promote the regeneration of damaged neural tissue, for instance, after a Spinal Cord Injury (SCI). In particular, aligned Polylactic Acid (PLA) microfibrils’ scaffolds are capable of supporting cells, promoting their survival and guiding their differentiation in neural lineage to repair the lesion. Despite its biocompatible nature, PLA is an electrically insulating material and thus it could be detrimental for increasingly common scaffolds’ electric functionalization, aimed at accelerating the cellular processes. In this context, the European RISEUP project aims to combine high intense microseconds pulses and DC stimulation with neurogenesis, supported by a PLA microfibrils’ scaffold.Methods: In this paper a numerical study on the effect of microfibrils’ scaffolds on the E-field distribution, in planar interdigitated electrodes, is presented. Realistic microfibrils’ 3D CAD models have been built to carry out a numerical dosimetry study, through Comsol Multiphysics software.Results: Under a voltage of 10 V, microfibrils redistribute the E-field values focalizing the field streamlines in the spaces between the fibers, allowing the field to pass and reach maximum values up to 100 kV/m and values comparable with the bare electrodes’ device (without fibers).Discussion: Globally the median E-field inside the scaffolded electrodes is the 90% of the nominal field, allowing an adequate cells’ exposure. |
| first_indexed | 2024-03-11T15:47:09Z |
| format | Article |
| id | doaj.art-7db0e0275d3f4dbb8276f73ce2d07e06 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-11T15:47:09Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-7db0e0275d3f4dbb8276f73ce2d07e062023-10-26T06:12:07ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-10-011110.3389/fbioe.2023.12644061264406Electric field bridging-effect in electrified microfibrils’ scaffoldsSara Fontana0Sara Fontana1Laura Caramazza2Laura Caramazza3Paolo Marracino4Irene Cuenca Ortolá5Micol Colella6Noemi Dolciotti7Alessandra Paffi8Fernando Gisbert Roca9Sergiy Ivashchenko10Jorge Más Estellés11Claudia Consales12Marco Balucani13Francesca Apollonio14Francesca Apollonio15Micaela Liberti16Micaela Liberti17BioEM Lab, Department of Information Engineering, Electronics and Telecommunications (DIET), Sapienza University of Rome, Rome, ItalyCenter for Life Nano- & Neuro-Science, Fondazione Istituto Italiano di Tecnologia (IIT), Rome, ItalyBioEM Lab, Department of Information Engineering, Electronics and Telecommunications (DIET), Sapienza University of Rome, Rome, ItalyCenter for Life Nano- & Neuro-Science, Fondazione Istituto Italiano di Tecnologia (IIT), Rome, ItalyRise Technology S.R.L, Rome, ItalyCenter for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, SpainBioEM Lab, Department of Information Engineering, Electronics and Telecommunications (DIET), Sapienza University of Rome, Rome, ItalyBioEM Lab, Department of Information Engineering, Electronics and Telecommunications (DIET), Sapienza University of Rome, Rome, ItalyBioEM Lab, Department of Information Engineering, Electronics and Telecommunications (DIET), Sapienza University of Rome, Rome, ItalyCenter for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, SpainCenter for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, SpainCenter for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Valencia, SpainDivision of Health Protection Technologies, ENEA-Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Rome, ItalyRise Technology S.R.L, Rome, ItalyBioEM Lab, Department of Information Engineering, Electronics and Telecommunications (DIET), Sapienza University of Rome, Rome, ItalyCenter for Life Nano- & Neuro-Science, Fondazione Istituto Italiano di Tecnologia (IIT), Rome, ItalyBioEM Lab, Department of Information Engineering, Electronics and Telecommunications (DIET), Sapienza University of Rome, Rome, ItalyCenter for Life Nano- & Neuro-Science, Fondazione Istituto Italiano di Tecnologia (IIT), Rome, ItalyIntroduction: The use of biocompatible scaffolds combined with the implantation of neural stem cells, is increasingly being investigated to promote the regeneration of damaged neural tissue, for instance, after a Spinal Cord Injury (SCI). In particular, aligned Polylactic Acid (PLA) microfibrils’ scaffolds are capable of supporting cells, promoting their survival and guiding their differentiation in neural lineage to repair the lesion. Despite its biocompatible nature, PLA is an electrically insulating material and thus it could be detrimental for increasingly common scaffolds’ electric functionalization, aimed at accelerating the cellular processes. In this context, the European RISEUP project aims to combine high intense microseconds pulses and DC stimulation with neurogenesis, supported by a PLA microfibrils’ scaffold.Methods: In this paper a numerical study on the effect of microfibrils’ scaffolds on the E-field distribution, in planar interdigitated electrodes, is presented. Realistic microfibrils’ 3D CAD models have been built to carry out a numerical dosimetry study, through Comsol Multiphysics software.Results: Under a voltage of 10 V, microfibrils redistribute the E-field values focalizing the field streamlines in the spaces between the fibers, allowing the field to pass and reach maximum values up to 100 kV/m and values comparable with the bare electrodes’ device (without fibers).Discussion: Globally the median E-field inside the scaffolded electrodes is the 90% of the nominal field, allowing an adequate cells’ exposure.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1264406/fulltissue engineeringbiocompatible scaffoldmicrofibrilselectric stimulationnumerical modelingdosimetry |
| spellingShingle | Sara Fontana Sara Fontana Laura Caramazza Laura Caramazza Paolo Marracino Irene Cuenca Ortolá Micol Colella Noemi Dolciotti Alessandra Paffi Fernando Gisbert Roca Sergiy Ivashchenko Jorge Más Estellés Claudia Consales Marco Balucani Francesca Apollonio Francesca Apollonio Micaela Liberti Micaela Liberti Electric field bridging-effect in electrified microfibrils’ scaffolds Frontiers in Bioengineering and Biotechnology tissue engineering biocompatible scaffold microfibrils electric stimulation numerical modeling dosimetry |
| title | Electric field bridging-effect in electrified microfibrils’ scaffolds |
| title_full | Electric field bridging-effect in electrified microfibrils’ scaffolds |
| title_fullStr | Electric field bridging-effect in electrified microfibrils’ scaffolds |
| title_full_unstemmed | Electric field bridging-effect in electrified microfibrils’ scaffolds |
| title_short | Electric field bridging-effect in electrified microfibrils’ scaffolds |
| title_sort | electric field bridging effect in electrified microfibrils scaffolds |
| topic | tissue engineering biocompatible scaffold microfibrils electric stimulation numerical modeling dosimetry |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1264406/full |
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