The effect of concentration and storage time on short-term storage of ram sperm
The present study was conducted to observe the effect of different dilution rates on ram sperm motion characters (motility, progressivity, velocity and average of speed), head diameter, mitochondrial membrane potential (MMP) and live intact acrosome rate during liquid storage for 96 h at 5ºC. Normos...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2022-11-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2954 |
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| author | Cemal DAYANIKLI Ebru SENGUL Bulent BULBUL Burcu USTUNER Zekariya NUR |
| author_facet | Cemal DAYANIKLI Ebru SENGUL Bulent BULBUL Burcu USTUNER Zekariya NUR |
| author_sort | Cemal DAYANIKLI |
| collection | DOAJ |
| description | The present study was conducted to observe the effect of different dilution rates on ram sperm motion characters (motility,
progressivity, velocity and average of speed), head diameter, mitochondrial membrane potential (MMP) and live intact acrosome rate
during liquid storage for 96 h at 5ºC. Normospermic semen samples that obtained from 4 adult rams were pooled. Pooled semen was split
into two aliquots; Low Dilution Rate (LDR) (400 million) and High Dilution Rate (HDR) (20 million) motile spermatozoa per insemination
dose/0.25 mL straw and were diluted with a skim milk-egg yolk-based extender. It was observed that the Total Motility (TM) and total
progressivity of the LDR group decreased more rapidly than HDR group over time, in general. From the 12th h to the 96th h; the rapid
progressivity (P<0.01), MMP (P<0.05), live sperm rates with intact acrosome (P<0.01) and straightness (STR) (P<0.001) of the HDR group
results were statistically better than LDR. It was concluded that the spermatological properties examined during storage were damaged at
different times and were affected by sperm concentration and also storage time. |
| first_indexed | 2024-03-13T04:41:28Z |
| format | Article |
| id | doaj.art-7db2d9ef2c9141f3bdeeff03889d2399 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:41:28Z |
| publishDate | 2022-11-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-7db2d9ef2c9141f3bdeeff03889d23992023-06-19T05:49:02ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512022-11-0128668169010.9775/kvfd.2022.276362954The effect of concentration and storage time on short-term storage of ram spermCemal DAYANIKLI0Ebru SENGUL1Bulent BULBUL2Burcu USTUNER3Zekariya NUR4Department of Breeding Techniques, Sheep Breeding Research Institute, TR - 10200 Bandırma, Balıkesir - TÜRKİYEDepartment of Breeding Techniques, Sheep Breeding Research Institute, TR - 10200 Bandırma, Balıkesir - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Dokuz Eylul University, TR- 35890 Kiraz, İzmir - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, TR- 16059 Görükle, Bursa - TÜRKİYEDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, TR- 16059 Görükle, Bursa - TÜRKİYEThe present study was conducted to observe the effect of different dilution rates on ram sperm motion characters (motility, progressivity, velocity and average of speed), head diameter, mitochondrial membrane potential (MMP) and live intact acrosome rate during liquid storage for 96 h at 5ºC. Normospermic semen samples that obtained from 4 adult rams were pooled. Pooled semen was split into two aliquots; Low Dilution Rate (LDR) (400 million) and High Dilution Rate (HDR) (20 million) motile spermatozoa per insemination dose/0.25 mL straw and were diluted with a skim milk-egg yolk-based extender. It was observed that the Total Motility (TM) and total progressivity of the LDR group decreased more rapidly than HDR group over time, in general. From the 12th h to the 96th h; the rapid progressivity (P<0.01), MMP (P<0.05), live sperm rates with intact acrosome (P<0.01) and straightness (STR) (P<0.001) of the HDR group results were statistically better than LDR. It was concluded that the spermatological properties examined during storage were damaged at different times and were affected by sperm concentration and also storage time.https://vetdergikafkas.org/pdf.php?id=2954acrosomeconcentrationkinematicsmmpmotilityramsemenextenderstorage |
| spellingShingle | Cemal DAYANIKLI Ebru SENGUL Bulent BULBUL Burcu USTUNER Zekariya NUR The effect of concentration and storage time on short-term storage of ram sperm Kafkas Universitesi Veteriner Fakültesi Dergisi acrosome concentration kinematics mmp motility ram semen extender storage |
| title | The effect of concentration and storage time on short-term storage of ram sperm |
| title_full | The effect of concentration and storage time on short-term storage of ram sperm |
| title_fullStr | The effect of concentration and storage time on short-term storage of ram sperm |
| title_full_unstemmed | The effect of concentration and storage time on short-term storage of ram sperm |
| title_short | The effect of concentration and storage time on short-term storage of ram sperm |
| title_sort | effect of concentration and storage time on short term storage of ram sperm |
| topic | acrosome concentration kinematics mmp motility ram semen extender storage |
| url | https://vetdergikafkas.org/pdf.php?id=2954 |
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