Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA
Abstract Biopolymer topology is critical for determining interactions inside cell environments, exemplified by DNA where its response to mechanical perturbation is as important as biochemical properties to its cellular roles. The dynamic structures of chiral biopolymers exhibit complex dependence wi...
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| Format: | Article |
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Nature Portfolio
2024-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-47126-6 |
| _version_ | 1797233466951073792 |
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| author | Jack W. Shepherd Sebastien Guilbaud Zhaokun Zhou Jamieson A. L. Howard Matthew Burman Charley Schaefer Adam Kerrigan Clare Steele-King Agnes Noy Mark C. Leake |
| author_facet | Jack W. Shepherd Sebastien Guilbaud Zhaokun Zhou Jamieson A. L. Howard Matthew Burman Charley Schaefer Adam Kerrigan Clare Steele-King Agnes Noy Mark C. Leake |
| author_sort | Jack W. Shepherd |
| collection | DOAJ |
| description | Abstract Biopolymer topology is critical for determining interactions inside cell environments, exemplified by DNA where its response to mechanical perturbation is as important as biochemical properties to its cellular roles. The dynamic structures of chiral biopolymers exhibit complex dependence with extension and torsion, however the physical mechanisms underpinning the emergence of structural motifs upon physiological twisting and stretching are poorly understood due to technological limitations in correlating force, torque and spatial localization information. We present COMBI-Tweez (Combined Optical and Magnetic BIomolecule TWEEZers), a transformative tool that overcomes these challenges by integrating optical trapping, time-resolved electromagnetic tweezers, and fluorescence microscopy, demonstrated on single DNA molecules, that can controllably form and visualise higher order structural motifs including plectonemes. This technology combined with cutting-edge MD simulations provides quantitative insight into complex dynamic structures relevant to DNA cellular processes and can be adapted to study a range of filamentous biopolymers. |
| first_indexed | 2024-04-24T16:16:37Z |
| format | Article |
| id | doaj.art-7db654a7a24440c7a4597f05ffd56ce8 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T16:16:37Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-7db654a7a24440c7a4597f05ffd56ce82024-03-31T11:24:42ZengNature PortfolioNature Communications2041-17232024-03-0115111510.1038/s41467-024-47126-6Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNAJack W. Shepherd0Sebastien Guilbaud1Zhaokun Zhou2Jamieson A. L. Howard3Matthew Burman4Charley Schaefer5Adam Kerrigan6Clare Steele-King7Agnes Noy8Mark C. Leake9School of Physics, Engineering and Technology, University of YorkSchool of Physics, Engineering and Technology, University of YorkGuangdong Provincial Key Lab of Robotics and Intelligent System, Shenzhen Institute of Advanced Technology, Chinese Academy of SciencesSchool of Physics, Engineering and Technology, University of YorkSchool of Physics, Engineering and Technology, University of YorkSchool of Physics, Engineering and Technology, University of YorkThe York-JEOL Nanocentre, University of YorkBioscience Technology Facility, University of YorkSchool of Physics, Engineering and Technology, University of YorkSchool of Physics, Engineering and Technology, University of YorkAbstract Biopolymer topology is critical for determining interactions inside cell environments, exemplified by DNA where its response to mechanical perturbation is as important as biochemical properties to its cellular roles. The dynamic structures of chiral biopolymers exhibit complex dependence with extension and torsion, however the physical mechanisms underpinning the emergence of structural motifs upon physiological twisting and stretching are poorly understood due to technological limitations in correlating force, torque and spatial localization information. We present COMBI-Tweez (Combined Optical and Magnetic BIomolecule TWEEZers), a transformative tool that overcomes these challenges by integrating optical trapping, time-resolved electromagnetic tweezers, and fluorescence microscopy, demonstrated on single DNA molecules, that can controllably form and visualise higher order structural motifs including plectonemes. This technology combined with cutting-edge MD simulations provides quantitative insight into complex dynamic structures relevant to DNA cellular processes and can be adapted to study a range of filamentous biopolymers.https://doi.org/10.1038/s41467-024-47126-6 |
| spellingShingle | Jack W. Shepherd Sebastien Guilbaud Zhaokun Zhou Jamieson A. L. Howard Matthew Burman Charley Schaefer Adam Kerrigan Clare Steele-King Agnes Noy Mark C. Leake Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA Nature Communications |
| title | Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA |
| title_full | Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA |
| title_fullStr | Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA |
| title_full_unstemmed | Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA |
| title_short | Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA |
| title_sort | correlating fluorescence microscopy optical and magnetic tweezers to study single chiral biopolymers such as dna |
| url | https://doi.org/10.1038/s41467-024-47126-6 |
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