Cysteine-Capped Hydrogels Incorporating Copper as Effective Antimicrobial Materials against Methicillin-Resistant <i>Staphylococcus aureus</i>
Methicillin-resistant <i>Staphylococcus aureus</i> (<i>S. aureus</i>) (MRSA) has become an alarming threat to public health, and infected soft tissue. Antibiotics are commonly used to treat skin infection with MRSA, but the inappropriate use of antibiotics runs a considerable...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/2/149 |
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| author | John Jackson Yang Yung-Chi Huang Tsung-Hsien Chuang Deron Raymond Herr Ming-Fa Hsieh Chun-Jen Huang Chun-Ming Huang |
| author_facet | John Jackson Yang Yung-Chi Huang Tsung-Hsien Chuang Deron Raymond Herr Ming-Fa Hsieh Chun-Jen Huang Chun-Ming Huang |
| author_sort | John Jackson Yang |
| collection | DOAJ |
| description | Methicillin-resistant <i>Staphylococcus aureus</i> (<i>S. aureus</i>) (MRSA) has become an alarming threat to public health, and infected soft tissue. Antibiotics are commonly used to treat skin infection with MRSA, but the inappropriate use of antibiotics runs a considerable risk of generating resistant <i>S. aureus</i>. In this study, we created a cysteine-capped hydrogel able to absorb and release copper, an ion with the capability of suppressing the growth of USA300, a community-acquired MRSA. The results of analysis of Fourier transform infrared spectroscopy (FTIR) revealed the binding of copper to a cysteine-capped hydrogel. The topical application of a cysteine-capped hydrogel binding with copper on USA300-infected skin wounds in the dorsal skin of Institute of Cancer Research (ICR) mice significantly enhanced wound healing, hindered the growth of USA300, and reduced the production of pro-inflammatory macrophage inflammatory protein 2-alpha (MIP-2) cytokine. Our work demonstrates a newly designed hydrogel that conjugates a cysteine molecule for copper binding. The cysteine-capped hydrogel can potentially chelate various antimicrobial metals as a novel wound dressing. |
| first_indexed | 2024-12-21T04:02:51Z |
| format | Article |
| id | doaj.art-7db7706a6dfc44ddba0e36f1ce2751eb |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-21T04:02:51Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7db7706a6dfc44ddba0e36f1ce2751eb2022-12-21T19:16:41ZengMDPI AGMicroorganisms2076-26072020-01-018214910.3390/microorganisms8020149microorganisms8020149Cysteine-Capped Hydrogels Incorporating Copper as Effective Antimicrobial Materials against Methicillin-Resistant <i>Staphylococcus aureus</i>John Jackson Yang0Yung-Chi Huang1Tsung-Hsien Chuang2Deron Raymond Herr3Ming-Fa Hsieh4Chun-Jen Huang5Chun-Ming Huang6Department of Life Sciences, National Central University, Taoyuan County 32001, TaiwanDepartment of Life Sciences, National Central University, Taoyuan County 32001, TaiwanImmunology Research Center, National Health Research Institutes (NHRI), Zhunan, Miaoli County 35053, TaiwanDepartment of Pharmacology, National University of Singapore, Singapore 117543, SingaporeDepartment of Biomedical Engineering, Chung Yuan Christian University, Taoyuan City 32023, TaiwanDepartment of Biomedical Sciences and Engineering, National Central University, Taoyuan County 32001, TaiwanDepartment of Biomedical Sciences and Engineering, National Central University, Taoyuan County 32001, TaiwanMethicillin-resistant <i>Staphylococcus aureus</i> (<i>S. aureus</i>) (MRSA) has become an alarming threat to public health, and infected soft tissue. Antibiotics are commonly used to treat skin infection with MRSA, but the inappropriate use of antibiotics runs a considerable risk of generating resistant <i>S. aureus</i>. In this study, we created a cysteine-capped hydrogel able to absorb and release copper, an ion with the capability of suppressing the growth of USA300, a community-acquired MRSA. The results of analysis of Fourier transform infrared spectroscopy (FTIR) revealed the binding of copper to a cysteine-capped hydrogel. The topical application of a cysteine-capped hydrogel binding with copper on USA300-infected skin wounds in the dorsal skin of Institute of Cancer Research (ICR) mice significantly enhanced wound healing, hindered the growth of USA300, and reduced the production of pro-inflammatory macrophage inflammatory protein 2-alpha (MIP-2) cytokine. Our work demonstrates a newly designed hydrogel that conjugates a cysteine molecule for copper binding. The cysteine-capped hydrogel can potentially chelate various antimicrobial metals as a novel wound dressing.https://www.mdpi.com/2076-2607/8/2/149antimicrobialcoppercysteine-capped hydrogels<i>s. aureus</i>skin wounds |
| spellingShingle | John Jackson Yang Yung-Chi Huang Tsung-Hsien Chuang Deron Raymond Herr Ming-Fa Hsieh Chun-Jen Huang Chun-Ming Huang Cysteine-Capped Hydrogels Incorporating Copper as Effective Antimicrobial Materials against Methicillin-Resistant <i>Staphylococcus aureus</i> Microorganisms antimicrobial copper cysteine-capped hydrogels <i>s. aureus</i> skin wounds |
| title | Cysteine-Capped Hydrogels Incorporating Copper as Effective Antimicrobial Materials against Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_full | Cysteine-Capped Hydrogels Incorporating Copper as Effective Antimicrobial Materials against Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_fullStr | Cysteine-Capped Hydrogels Incorporating Copper as Effective Antimicrobial Materials against Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Cysteine-Capped Hydrogels Incorporating Copper as Effective Antimicrobial Materials against Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_short | Cysteine-Capped Hydrogels Incorporating Copper as Effective Antimicrobial Materials against Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_sort | cysteine capped hydrogels incorporating copper as effective antimicrobial materials against methicillin resistant i staphylococcus aureus i |
| topic | antimicrobial copper cysteine-capped hydrogels <i>s. aureus</i> skin wounds |
| url | https://www.mdpi.com/2076-2607/8/2/149 |
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