Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Oryza sativa L.)
The ALOG (Arabidopsis LSH1 and Oryza G1) family proteins, namely, DUF640 domain-containing proteins, have been reported to function as transcription factors in various plants. However, the understanding of the response and function of ALOG family genes during reproductive development and under abiot...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2024.1381690/full |
| _version_ | 1797219255632003072 |
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| author | Zhiyuan Liu Zhenjiang Fan Lei Wang Siyue Zhang Weichen Xu Sijie Zhao Sijia Fang Mei Liu Sackitey Mark Kofi Shuangxi Zhang Ningning Kang Hao Ai Ruining Li Tingting Feng Shuya Wei Heming Zhao |
| author_facet | Zhiyuan Liu Zhenjiang Fan Lei Wang Siyue Zhang Weichen Xu Sijie Zhao Sijia Fang Mei Liu Sackitey Mark Kofi Shuangxi Zhang Ningning Kang Hao Ai Ruining Li Tingting Feng Shuya Wei Heming Zhao |
| author_sort | Zhiyuan Liu |
| collection | DOAJ |
| description | The ALOG (Arabidopsis LSH1 and Oryza G1) family proteins, namely, DUF640 domain-containing proteins, have been reported to function as transcription factors in various plants. However, the understanding of the response and function of ALOG family genes during reproductive development and under abiotic stress is still largely limited. In this study, we comprehensively analyzed the structural characteristics of ALOG family proteins and their expression profiles during inflorescence development and under abiotic stress in rice. The results showed that OsG1/OsG1L1/2/3/4/5/6/7/8/9 all had four conserved helical structures and an inserted Zinc-Ribbon (ZnR), the other four proteins OsG1L10/11/12/13 lacked complete Helix-1 and Helix-2. In the ALOG gene promoters, there were abundant cis-acting elements, including ABA, MeJA, and drought-responsive elements. Most ALOG genes show a decrease in expression levels within 24 h under ABA and drought treatments, while OsG1L2 expression levels show an upregulated trend under ABA and drought treatments. The expression analysis at different stages of inflorescence development indicated that OsG1L1/2/3/8/11 were mainly expressed in the P1 stage; in the P4 stage, OsG1/OsG1L4/5/9/12 had a higher expression level. These results lay a good foundation for further studying the expression of rice ALOG family genes under abiotic stresses, and provide important experimental support for their functional research. |
| first_indexed | 2024-04-24T12:30:45Z |
| format | Article |
| id | doaj.art-7db8c59f9e084e09bb19283664567796 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-24T12:30:45Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-7db8c59f9e084e09bb192836645677962024-04-08T04:50:26ZengFrontiers Media S.A.Frontiers in Genetics1664-80212024-04-011510.3389/fgene.2024.13816901381690Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Oryza sativa L.)Zhiyuan Liu0Zhenjiang Fan1Lei Wang2Siyue Zhang3Weichen Xu4Sijie Zhao5Sijia Fang6Mei Liu7Sackitey Mark Kofi8Shuangxi Zhang9Ningning Kang10Hao Ai11Ruining Li12Tingting Feng13Shuya Wei14Heming Zhao15Country Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaCollege of Bioengineering, Wuhan Polytechnic, Wuhan, ChinaCountry Center for Crop Biotechnology, College of Agriculture, Anhui Science and Technology University, Fengyang, ChinaThe ALOG (Arabidopsis LSH1 and Oryza G1) family proteins, namely, DUF640 domain-containing proteins, have been reported to function as transcription factors in various plants. However, the understanding of the response and function of ALOG family genes during reproductive development and under abiotic stress is still largely limited. In this study, we comprehensively analyzed the structural characteristics of ALOG family proteins and their expression profiles during inflorescence development and under abiotic stress in rice. The results showed that OsG1/OsG1L1/2/3/4/5/6/7/8/9 all had four conserved helical structures and an inserted Zinc-Ribbon (ZnR), the other four proteins OsG1L10/11/12/13 lacked complete Helix-1 and Helix-2. In the ALOG gene promoters, there were abundant cis-acting elements, including ABA, MeJA, and drought-responsive elements. Most ALOG genes show a decrease in expression levels within 24 h under ABA and drought treatments, while OsG1L2 expression levels show an upregulated trend under ABA and drought treatments. The expression analysis at different stages of inflorescence development indicated that OsG1L1/2/3/8/11 were mainly expressed in the P1 stage; in the P4 stage, OsG1/OsG1L4/5/9/12 had a higher expression level. These results lay a good foundation for further studying the expression of rice ALOG family genes under abiotic stresses, and provide important experimental support for their functional research.https://www.frontiersin.org/articles/10.3389/fgene.2024.1381690/fullriceALOG familyexpression analysisinflorescence developmentabiotic stress |
| spellingShingle | Zhiyuan Liu Zhenjiang Fan Lei Wang Siyue Zhang Weichen Xu Sijie Zhao Sijia Fang Mei Liu Sackitey Mark Kofi Shuangxi Zhang Ningning Kang Hao Ai Ruining Li Tingting Feng Shuya Wei Heming Zhao Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Oryza sativa L.) Frontiers in Genetics rice ALOG family expression analysis inflorescence development abiotic stress |
| title | Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Oryza sativa L.) |
| title_full | Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Oryza sativa L.) |
| title_fullStr | Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Oryza sativa L.) |
| title_full_unstemmed | Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Oryza sativa L.) |
| title_short | Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Oryza sativa L.) |
| title_sort | expression profiling of alog family genes during inflorescence development and abiotic stress responses in rice oryza sativa l |
| topic | rice ALOG family expression analysis inflorescence development abiotic stress |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2024.1381690/full |
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