Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update
The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began as a cluster of pneumonia cases in Wuhan, China before spreading to over 200 countries and territories on six continents in less than six months. Despite rigorous global containment a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/1/53 |
| _version_ | 1827698707723714560 |
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| author | Choo Yee Yu Kok Gan Chan Chan Yean Yean Geik Yong Ang |
| author_facet | Choo Yee Yu Kok Gan Chan Chan Yean Yean Geik Yong Ang |
| author_sort | Choo Yee Yu |
| collection | DOAJ |
| description | The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began as a cluster of pneumonia cases in Wuhan, China before spreading to over 200 countries and territories on six continents in less than six months. Despite rigorous global containment and quarantine efforts to limit the transmission of the virus, COVID-19 cases and deaths have continued to increase, leaving devastating impacts on the lives of many with far-reaching effects on the global society, economy and healthcare system. With over 43 million cases and 1.1 million deaths recorded worldwide, accurate and rapid diagnosis continues to be a cornerstone of pandemic control. In this review, we aim to present an objective overview of the latest nucleic acid-based diagnostic tests for the detection of SARS-CoV-2 that have been authorized by the Food and Drug Administration (FDA) under emergency use authorization (EUA) as of 31 October 2020. We systematically summarize and compare the principles, technologies, protocols and performance characteristics of amplification- and sequencing-based tests that have become alternatives to the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel. We highlight the notable features of the tests including authorized settings, along with the advantages and disadvantages of the tests. We conclude with a brief discussion on the current challenges and future perspectives of COVID-19 diagnostics. |
| first_indexed | 2024-03-10T13:34:56Z |
| format | Article |
| id | doaj.art-7dba1c80f02946c1963fbbff4d54a489 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T13:34:56Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-7dba1c80f02946c1963fbbff4d54a4892023-11-21T07:39:35ZengMDPI AGDiagnostics2075-44182021-01-011115310.3390/diagnostics11010053Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An UpdateChoo Yee Yu0Kok Gan Chan1Chan Yean Yean2Geik Yong Ang3Independent Researcher, Kuala Lumpur 51200, MalaysiaDivision of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, MalaysiaDepartment of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kota Bharu 16150, MalaysiaFaculty of Sports Science and Recreation, Universiti Teknologi MARA, Shah Alam 40450, MalaysiaThe coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began as a cluster of pneumonia cases in Wuhan, China before spreading to over 200 countries and territories on six continents in less than six months. Despite rigorous global containment and quarantine efforts to limit the transmission of the virus, COVID-19 cases and deaths have continued to increase, leaving devastating impacts on the lives of many with far-reaching effects on the global society, economy and healthcare system. With over 43 million cases and 1.1 million deaths recorded worldwide, accurate and rapid diagnosis continues to be a cornerstone of pandemic control. In this review, we aim to present an objective overview of the latest nucleic acid-based diagnostic tests for the detection of SARS-CoV-2 that have been authorized by the Food and Drug Administration (FDA) under emergency use authorization (EUA) as of 31 October 2020. We systematically summarize and compare the principles, technologies, protocols and performance characteristics of amplification- and sequencing-based tests that have become alternatives to the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel. We highlight the notable features of the tests including authorized settings, along with the advantages and disadvantages of the tests. We conclude with a brief discussion on the current challenges and future perspectives of COVID-19 diagnostics.https://www.mdpi.com/2075-4418/11/1/53COVID-19rapid testRT-PCRisothermallateral flowLAMP |
| spellingShingle | Choo Yee Yu Kok Gan Chan Chan Yean Yean Geik Yong Ang Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update Diagnostics COVID-19 rapid test RT-PCR isothermal lateral flow LAMP |
| title | Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update |
| title_full | Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update |
| title_fullStr | Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update |
| title_full_unstemmed | Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update |
| title_short | Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update |
| title_sort | nucleic acid based diagnostic tests for the detection sars cov 2 an update |
| topic | COVID-19 rapid test RT-PCR isothermal lateral flow LAMP |
| url | https://www.mdpi.com/2075-4418/11/1/53 |
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