Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001
For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the an...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2019-09-01
|
| Series: | Synthetic and Systems Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X19300444 |
| _version_ | 1797204054741352448 |
|---|---|
| author | Sagarika Devi Heiko T. Kiesewalter Renátó Kovács Jens Christian Frisvad Tilmann Weber Thomas Ostenfeld Larsen Ákos T. Kovács Ling Ding |
| author_facet | Sagarika Devi Heiko T. Kiesewalter Renátó Kovács Jens Christian Frisvad Tilmann Weber Thomas Ostenfeld Larsen Ákos T. Kovács Ling Ding |
| author_sort | Sagarika Devi |
| collection | DOAJ |
| description | For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi. We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans. In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals. Keywords: Bacillus velezensis, Candida albicans, Secondary metabolites, Biocontrol, Biofilm |
| first_indexed | 2024-04-24T08:29:08Z |
| format | Article |
| id | doaj.art-7dba9b425a0f47ce94ebbeda1c3624a4 |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2024-04-24T08:29:08Z |
| publishDate | 2019-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-7dba9b425a0f47ce94ebbeda1c3624a42024-04-16T20:37:00ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2019-09-0143142149Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001Sagarika Devi0Heiko T. Kiesewalter1Renátó Kovács2Jens Christian Frisvad3Tilmann Weber4Thomas Ostenfeld Larsen5Ákos T. Kovács6Ling Ding7Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800, Kgs Lyngby, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, 2800, Kgs Lyngby, DenmarkDepartment of Medical Microbiology, Faculty of Medicine, University of Debrecen, 4032, Debrecen, HungaryDepartment of Biotechnology and Biomedicine, Technical University of Denmark, 2800, Kgs Lyngby, DenmarkCenter for Biosustainability, Technical University of Denmark, 2800, Kgs Lyngby, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, 2800, Kgs Lyngby, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, 2800, Kgs Lyngby, Denmark; Corresponding author.Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800, Kgs Lyngby, Denmark; Corresponding author.For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi. We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans. In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals. Keywords: Bacillus velezensis, Candida albicans, Secondary metabolites, Biocontrol, Biofilmhttp://www.sciencedirect.com/science/article/pii/S2405805X19300444 |
| spellingShingle | Sagarika Devi Heiko T. Kiesewalter Renátó Kovács Jens Christian Frisvad Tilmann Weber Thomas Ostenfeld Larsen Ákos T. Kovács Ling Ding Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001 Synthetic and Systems Biotechnology |
| title | Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001 |
| title_full | Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001 |
| title_fullStr | Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001 |
| title_full_unstemmed | Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001 |
| title_short | Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001 |
| title_sort | depiction of secondary metabolites and antifungal activity of bacillus velezensis dtu001 |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X19300444 |
| work_keys_str_mv | AT sagarikadevi depictionofsecondarymetabolitesandantifungalactivityofbacillusvelezensisdtu001 AT heikotkiesewalter depictionofsecondarymetabolitesandantifungalactivityofbacillusvelezensisdtu001 AT renatokovacs depictionofsecondarymetabolitesandantifungalactivityofbacillusvelezensisdtu001 AT jenschristianfrisvad depictionofsecondarymetabolitesandantifungalactivityofbacillusvelezensisdtu001 AT tilmannweber depictionofsecondarymetabolitesandantifungalactivityofbacillusvelezensisdtu001 AT thomasostenfeldlarsen depictionofsecondarymetabolitesandantifungalactivityofbacillusvelezensisdtu001 AT akostkovacs depictionofsecondarymetabolitesandantifungalactivityofbacillusvelezensisdtu001 AT lingding depictionofsecondarymetabolitesandantifungalactivityofbacillusvelezensisdtu001 |