Formulation of polymeric nanoparticles loaded sorafenib; evaluation of cytotoxicity, molecular evaluation, and gene expression studies in lung and breast cancer cell lines
Sorafenib (SFB) is an anticancer drug with sparingly water solubility and reduced bioavailability. Nanoformulation of SFB can increase its dissolution rate and solubility. The current study aimed to formulate SFB in nanoparticles to improve their solubility. The sorafenib nanoparticles (SFB-PNs) wer...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-02-01
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| Series: | Nanotechnology Reviews |
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| Online Access: | https://doi.org/10.1515/ntrev-2022-0058 |
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| author | Abdellatif Ahmed A. H. Ali Asmaa T. Bouazzaoui Abdellatif Alsharidah Mansour Al Rugaie Osamah Tolba Nahla Sameh |
| author_facet | Abdellatif Ahmed A. H. Ali Asmaa T. Bouazzaoui Abdellatif Alsharidah Mansour Al Rugaie Osamah Tolba Nahla Sameh |
| author_sort | Abdellatif Ahmed A. H. |
| collection | DOAJ |
| description | Sorafenib (SFB) is an anticancer drug with sparingly water solubility and reduced bioavailability. Nanoformulation of SFB can increase its dissolution rate and solubility. The current study aimed to formulate SFB in nanoparticles to improve their solubility. The sorafenib nanoparticles (SFB-PNs) were synthesized using the solvent evaporation method, then evaluated for their particle size, polydispersity index (PDI), zeta-potential, morphological structure, and entrapment efficiency (EE%). Further, the anticancer efficacy in A549 and Michigan Cancer Foundation-7 (MCF-7) cancer cell lines was evaluated. The SFB-NPs were uniform in size, which have 389.7 ± 16.49 nm, PDI of 0.703 ± 0.12, and zeta-potential of −13.5 ± 12.1 mV, whereas transmission electron microscopy showed a well-identified spherical particle. The EE% was found to be 73.7 ± 0.8%. SFB-NPs inhibited the cell growth by 50% after 48 h incubation, with IC50 of 2.26 and 1.28 µg/mL in A549 and MCF-7, respectively. Additionally, SFB-NPs showed a significant decrease (P < 0.05) in p21, and stathmin-1 gene expression levels in both cell lines. Moreover, SFB-NPs showed a significant increase in DNA damage of 25.50 and 26.75% in A549 and MCF-7, respectively. The results indicate that SFB-NPs are a potential candidate with an effective anticancer agent compared with free drugs. |
| first_indexed | 2024-04-12T02:48:42Z |
| format | Article |
| id | doaj.art-7dbb37eea9d243fd8324c61177f92348 |
| institution | Directory Open Access Journal |
| issn | 2191-9097 |
| language | English |
| last_indexed | 2024-04-12T02:48:42Z |
| publishDate | 2022-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Nanotechnology Reviews |
| spelling | doaj.art-7dbb37eea9d243fd8324c61177f923482022-12-22T03:51:05ZengDe GruyterNanotechnology Reviews2191-90972022-02-01111987100410.1515/ntrev-2022-0058Formulation of polymeric nanoparticles loaded sorafenib; evaluation of cytotoxicity, molecular evaluation, and gene expression studies in lung and breast cancer cell linesAbdellatif Ahmed A. H.0Ali Asmaa T.1Bouazzaoui Abdellatif2Alsharidah Mansour3Al Rugaie Osamah4Tolba Nahla Sameh5Department of Pharmaceutics, College of Pharmacy, Qassim University, Qassim 51452, Saudi ArabiaDepartment of Biochemistry, Faculty of Pharmacy, Nahda University, Beni-Suef, EgyptDepartment of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Physiology, College of Medicine, Qassim University, Buraydah 51452, Saudi ArabiaDepartment of Basic Medical Sciences, College of Medicine and Medical Sciences, Qassim University, Unaizah, P.O. Box 991, Al Qassim 51911, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, University of Sadat City, Sadat City 32897, EgyptSorafenib (SFB) is an anticancer drug with sparingly water solubility and reduced bioavailability. Nanoformulation of SFB can increase its dissolution rate and solubility. The current study aimed to formulate SFB in nanoparticles to improve their solubility. The sorafenib nanoparticles (SFB-PNs) were synthesized using the solvent evaporation method, then evaluated for their particle size, polydispersity index (PDI), zeta-potential, morphological structure, and entrapment efficiency (EE%). Further, the anticancer efficacy in A549 and Michigan Cancer Foundation-7 (MCF-7) cancer cell lines was evaluated. The SFB-NPs were uniform in size, which have 389.7 ± 16.49 nm, PDI of 0.703 ± 0.12, and zeta-potential of −13.5 ± 12.1 mV, whereas transmission electron microscopy showed a well-identified spherical particle. The EE% was found to be 73.7 ± 0.8%. SFB-NPs inhibited the cell growth by 50% after 48 h incubation, with IC50 of 2.26 and 1.28 µg/mL in A549 and MCF-7, respectively. Additionally, SFB-NPs showed a significant decrease (P < 0.05) in p21, and stathmin-1 gene expression levels in both cell lines. Moreover, SFB-NPs showed a significant increase in DNA damage of 25.50 and 26.75% in A549 and MCF-7, respectively. The results indicate that SFB-NPs are a potential candidate with an effective anticancer agent compared with free drugs.https://doi.org/10.1515/ntrev-2022-0058sorafenibnanoparticlesa549 cell linesmcf-7 cell linesanticancer activities |
| spellingShingle | Abdellatif Ahmed A. H. Ali Asmaa T. Bouazzaoui Abdellatif Alsharidah Mansour Al Rugaie Osamah Tolba Nahla Sameh Formulation of polymeric nanoparticles loaded sorafenib; evaluation of cytotoxicity, molecular evaluation, and gene expression studies in lung and breast cancer cell lines Nanotechnology Reviews sorafenib nanoparticles a549 cell lines mcf-7 cell lines anticancer activities |
| title | Formulation of polymeric nanoparticles loaded sorafenib; evaluation of cytotoxicity, molecular evaluation, and gene expression studies in lung and breast cancer cell lines |
| title_full | Formulation of polymeric nanoparticles loaded sorafenib; evaluation of cytotoxicity, molecular evaluation, and gene expression studies in lung and breast cancer cell lines |
| title_fullStr | Formulation of polymeric nanoparticles loaded sorafenib; evaluation of cytotoxicity, molecular evaluation, and gene expression studies in lung and breast cancer cell lines |
| title_full_unstemmed | Formulation of polymeric nanoparticles loaded sorafenib; evaluation of cytotoxicity, molecular evaluation, and gene expression studies in lung and breast cancer cell lines |
| title_short | Formulation of polymeric nanoparticles loaded sorafenib; evaluation of cytotoxicity, molecular evaluation, and gene expression studies in lung and breast cancer cell lines |
| title_sort | formulation of polymeric nanoparticles loaded sorafenib evaluation of cytotoxicity molecular evaluation and gene expression studies in lung and breast cancer cell lines |
| topic | sorafenib nanoparticles a549 cell lines mcf-7 cell lines anticancer activities |
| url | https://doi.org/10.1515/ntrev-2022-0058 |
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