DNA fingerprinting with homologous multilocus probes and search for DNA markers associated with yield attributes in silkworm, Bombyx mori
RFLP clones harbouring multi-copy DNA sequences were isolated from the Pst I sub-genomic library of the indigenous silkworm race, Nistari, and were used for DNA fingerprinting studies in 13 stocks of silkworm, Bombyx mori L. Six multilocus probes produced 180 RFLP markers that showed a high level (9...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2002-09-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-200203-0001_DNA_fingerprinting_with_homologous_multilocus_probes_and_search_for_DNA_markers_associated_with_yield_attribute.php |
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| author | Balakathiresan N. SETHURAMAN Thampiyath P. MOHANDAS Shankar N. CHATTERJEE |
| author_facet | Balakathiresan N. SETHURAMAN Thampiyath P. MOHANDAS Shankar N. CHATTERJEE |
| author_sort | Balakathiresan N. SETHURAMAN |
| collection | DOAJ |
| description | RFLP clones harbouring multi-copy DNA sequences were isolated from the Pst I sub-genomic library of the indigenous silkworm race, Nistari, and were used for DNA fingerprinting studies in 13 stocks of silkworm, Bombyx mori L. Six multilocus probes produced 180 RFLP markers that showed a high level (98%) of polymorphism and are highly useful in molecular mapping, genotype characterization and marker assisted selection (MAS). The dendrogram derived from UPGMA analysis clearly divides the 13 silkworm stocks into two major clusters: high- and low-yield stocks. Furthermore, adopting multiple regression analyses, the RFLP marker(s) associated with characters of economic importance were identified, a first of its kind for any species of insect of commercial importance. The results obtained create an opportunity of using germplasm stocks directly for isolating specific RFLP band(s) and use it for MAS in breeding programs. |
| first_indexed | 2024-12-20T04:45:05Z |
| format | Article |
| id | doaj.art-7dc2326e53f4434fb7d55e51b4a41e3b |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-20T04:45:05Z |
| publishDate | 2002-09-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-7dc2326e53f4434fb7d55e51b4a41e3b2022-12-21T19:53:01ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292002-09-0199326727610.14411/eje.2002.037eje-200203-0001DNA fingerprinting with homologous multilocus probes and search for DNA markers associated with yield attributes in silkworm, Bombyx moriBalakathiresan N. SETHURAMAN0Thampiyath P. MOHANDAS1Shankar N. CHATTERJEESeriBiotech Lab, Central Silk Board, Campus-Kodathi, Bangalore 560035, Karnataka, IndiaSeriBiotech Lab, Central Silk Board, Campus-Kodathi, Bangalore 560035, Karnataka, IndiaRFLP clones harbouring multi-copy DNA sequences were isolated from the Pst I sub-genomic library of the indigenous silkworm race, Nistari, and were used for DNA fingerprinting studies in 13 stocks of silkworm, Bombyx mori L. Six multilocus probes produced 180 RFLP markers that showed a high level (98%) of polymorphism and are highly useful in molecular mapping, genotype characterization and marker assisted selection (MAS). The dendrogram derived from UPGMA analysis clearly divides the 13 silkworm stocks into two major clusters: high- and low-yield stocks. Furthermore, adopting multiple regression analyses, the RFLP marker(s) associated with characters of economic importance were identified, a first of its kind for any species of insect of commercial importance. The results obtained create an opportunity of using germplasm stocks directly for isolating specific RFLP band(s) and use it for MAS in breeding programs.https://www.eje.cz/artkey/eje-200203-0001_DNA_fingerprinting_with_homologous_multilocus_probes_and_search_for_DNA_markers_associated_with_yield_attribute.phpbombyx moridna fingerprintingmultilocus probesrestriction fragment length polymorphismrflp |
| spellingShingle | Balakathiresan N. SETHURAMAN Thampiyath P. MOHANDAS Shankar N. CHATTERJEE DNA fingerprinting with homologous multilocus probes and search for DNA markers associated with yield attributes in silkworm, Bombyx mori European Journal of Entomology bombyx mori dna fingerprinting multilocus probes restriction fragment length polymorphism rflp |
| title | DNA fingerprinting with homologous multilocus probes and search for DNA markers associated with yield attributes in silkworm, Bombyx mori |
| title_full | DNA fingerprinting with homologous multilocus probes and search for DNA markers associated with yield attributes in silkworm, Bombyx mori |
| title_fullStr | DNA fingerprinting with homologous multilocus probes and search for DNA markers associated with yield attributes in silkworm, Bombyx mori |
| title_full_unstemmed | DNA fingerprinting with homologous multilocus probes and search for DNA markers associated with yield attributes in silkworm, Bombyx mori |
| title_short | DNA fingerprinting with homologous multilocus probes and search for DNA markers associated with yield attributes in silkworm, Bombyx mori |
| title_sort | dna fingerprinting with homologous multilocus probes and search for dna markers associated with yield attributes in silkworm bombyx mori |
| topic | bombyx mori dna fingerprinting multilocus probes restriction fragment length polymorphism rflp |
| url | https://www.eje.cz/artkey/eje-200203-0001_DNA_fingerprinting_with_homologous_multilocus_probes_and_search_for_DNA_markers_associated_with_yield_attribute.php |
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