Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from <em>Catharanthus </em><em>r</em><em>oseus</em> Leaves
In the present study, an improved method termed negative-pressure cavitation extraction (NPCE) followed by reverse phase high-performance liquid chromatography (RP-HPLC) was developed for the extraction and quantification of vindoline (VDL), catharanthine (CTR), vincristine (VCR) and vinblastine (VL...
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| Format: | Article |
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MDPI AG
2012-07-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/8/8742 |
| _version_ | 1818175430541180928 |
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| author | Yuangang Zu Xiaorui Guo Yujie Fu Meng Luo Wei Wang Liuqing Yang Fansong Mu |
| author_facet | Yuangang Zu Xiaorui Guo Yujie Fu Meng Luo Wei Wang Liuqing Yang Fansong Mu |
| author_sort | Yuangang Zu |
| collection | DOAJ |
| description | In the present study, an improved method termed negative-pressure cavitation extraction (NPCE) followed by reverse phase high-performance liquid chromatography (RP-HPLC) was developed for the extraction and quantification of vindoline (VDL), catharanthine (CTR), vincristine (VCR) and vinblastine (VLB) from <em>Catharanthus roseus </em>leaves. The optimized method employed 60-mesh particles, 80% ethanol, a negative pressure of −0.075 MPa, a solid to liquid ratio of 1:20, 30 min of extraction and three extraction cycles. Under these optimized conditions, the extraction yields of VDL, CTR, VCR and VLB are 0.5783, 0.2843, 0.018 and 0.126 mg/g DW, respectively. These extraction yields are equivalent to those from the well-known ultrasonic extraction method and higher than the yields from maceration extraction and heating reflux extraction. Our results suggest that NPCE-RP-HPLC represents an excellent alternative for the extraction and quantification of vinca alkaloids for pilot- and industrial-scale applications. |
| first_indexed | 2024-12-11T20:00:10Z |
| format | Article |
| id | doaj.art-7dc2bfa91d8c4fabb48a1f6bd6bff464 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T20:00:10Z |
| publishDate | 2012-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7dc2bfa91d8c4fabb48a1f6bd6bff4642022-12-22T00:52:33ZengMDPI AGMolecules1420-30492012-07-011788742875210.3390/molecules17088742Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from <em>Catharanthus </em><em>r</em><em>oseus</em> LeavesYuangang ZuXiaorui GuoYujie FuMeng LuoWei WangLiuqing YangFansong MuIn the present study, an improved method termed negative-pressure cavitation extraction (NPCE) followed by reverse phase high-performance liquid chromatography (RP-HPLC) was developed for the extraction and quantification of vindoline (VDL), catharanthine (CTR), vincristine (VCR) and vinblastine (VLB) from <em>Catharanthus roseus </em>leaves. The optimized method employed 60-mesh particles, 80% ethanol, a negative pressure of −0.075 MPa, a solid to liquid ratio of 1:20, 30 min of extraction and three extraction cycles. Under these optimized conditions, the extraction yields of VDL, CTR, VCR and VLB are 0.5783, 0.2843, 0.018 and 0.126 mg/g DW, respectively. These extraction yields are equivalent to those from the well-known ultrasonic extraction method and higher than the yields from maceration extraction and heating reflux extraction. Our results suggest that NPCE-RP-HPLC represents an excellent alternative for the extraction and quantification of vinca alkaloids for pilot- and industrial-scale applications.http://www.mdpi.com/1420-3049/17/8/8742<em>Catharanthus roseus</em>vinca alkaloidsnegative-pressure cavitation extraction |
| spellingShingle | Yuangang Zu Xiaorui Guo Yujie Fu Meng Luo Wei Wang Liuqing Yang Fansong Mu Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from <em>Catharanthus </em><em>r</em><em>oseus</em> Leaves Molecules <em>Catharanthus roseus</em> vinca alkaloids negative-pressure cavitation extraction |
| title | Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from <em>Catharanthus </em><em>r</em><em>oseus</em> Leaves |
| title_full | Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from <em>Catharanthus </em><em>r</em><em>oseus</em> Leaves |
| title_fullStr | Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from <em>Catharanthus </em><em>r</em><em>oseus</em> Leaves |
| title_full_unstemmed | Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from <em>Catharanthus </em><em>r</em><em>oseus</em> Leaves |
| title_short | Negative-Pressure Cavitation Extraction of Four Main Vinca Alkaloids from <em>Catharanthus </em><em>r</em><em>oseus</em> Leaves |
| title_sort | negative pressure cavitation extraction of four main vinca alkaloids from lt em gt catharanthus lt em gt lt em gt r lt em gt lt em gt oseus lt em gt leaves |
| topic | <em>Catharanthus roseus</em> vinca alkaloids negative-pressure cavitation extraction |
| url | http://www.mdpi.com/1420-3049/17/8/8742 |
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