Isolating and Identifying One Strain with Lead-Tolerant Fungus and Preliminary Study on Its Capability of Biosorption to Pb<sup>2+</sup>
To obtain lead-resistant microorganisms as potential strains for bioremediation, in this study, a strain of fungus with high resistance to lead was isolated and domesticated from lead-contaminated soil, which was cultured and molecularly biologically identified as the genus Sarocladium Pb-9 (GenBank...
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MDPI AG
2024-12-01
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| author | Wanna Li Liujing Zhao Cui Liu Xingpeng Bai Chenyuan Xu Fengqiu An Feilong Sun |
| author_facet | Wanna Li Liujing Zhao Cui Liu Xingpeng Bai Chenyuan Xu Fengqiu An Feilong Sun |
| author_sort | Wanna Li |
| collection | DOAJ |
| description | To obtain lead-resistant microorganisms as potential strains for bioremediation, in this study, a strain of fungus with high resistance to lead was isolated and domesticated from lead-contaminated soil, which was cultured and molecularly biologically identified as the genus Sarocladium Pb-9 (GenBank No. MK372219). The optimal incubation time of strain Pb-9 was 96 h, the optimal incubation temperature was 25 °C, and the optimal incubation pH was 7. The strain Pb-9 had a good adsorption effect on Pb<sup>2+</sup> at a lead concentration of 2000 mg/L; scanning electron microscopy (SEM) observed that the spores of the Pb-9 strain appeared to be wrinkled and deformed under Pb<sup>2+</sup> stress, and XRD analysis showed that the mycelium of Pb-9 adsorbed Pb<sup>2+</sup>; Fourier transform infrared spectroscopy (FTIR) analysis showed that the Pb-9 strain might produce substances such as esters and polysaccharides under the treatment of different Pb<sup>2+</sup> concentrations. The above results showed that strain Pb-9 has good resistance and adsorption capacity to lead. Therefore, it has potential application value in the bioremediation of environmental heavy metal pollution, and this study provides a fundamental basis for the bioremediation of lead pollution in the environment. |
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| language | English |
| last_indexed | 2025-02-17T12:42:40Z |
| publishDate | 2024-12-01 |
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| spelling | doaj.art-7dc72b976e644a3ab1f28eea9eba9ed82024-12-27T14:12:08ZengMDPI AGBiology2079-77372024-12-011312105310.3390/biology13121053Isolating and Identifying One Strain with Lead-Tolerant Fungus and Preliminary Study on Its Capability of Biosorption to Pb<sup>2+</sup>Wanna Li0Liujing Zhao1Cui Liu2Xingpeng Bai3Chenyuan Xu4Fengqiu An5Feilong Sun6School of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710600, ChinaSchool of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710600, ChinaSchool of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710600, ChinaSchool of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710600, ChinaSchool of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710600, ChinaSchool of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710600, ChinaSchool of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an 710600, ChinaTo obtain lead-resistant microorganisms as potential strains for bioremediation, in this study, a strain of fungus with high resistance to lead was isolated and domesticated from lead-contaminated soil, which was cultured and molecularly biologically identified as the genus Sarocladium Pb-9 (GenBank No. MK372219). The optimal incubation time of strain Pb-9 was 96 h, the optimal incubation temperature was 25 °C, and the optimal incubation pH was 7. The strain Pb-9 had a good adsorption effect on Pb<sup>2+</sup> at a lead concentration of 2000 mg/L; scanning electron microscopy (SEM) observed that the spores of the Pb-9 strain appeared to be wrinkled and deformed under Pb<sup>2+</sup> stress, and XRD analysis showed that the mycelium of Pb-9 adsorbed Pb<sup>2+</sup>; Fourier transform infrared spectroscopy (FTIR) analysis showed that the Pb-9 strain might produce substances such as esters and polysaccharides under the treatment of different Pb<sup>2+</sup> concentrations. The above results showed that strain Pb-9 has good resistance and adsorption capacity to lead. Therefore, it has potential application value in the bioremediation of environmental heavy metal pollution, and this study provides a fundamental basis for the bioremediation of lead pollution in the environment.https://www.mdpi.com/2079-7737/13/12/1053leadSarocladiumSEMXRDFTIR |
| spellingShingle | Wanna Li Liujing Zhao Cui Liu Xingpeng Bai Chenyuan Xu Fengqiu An Feilong Sun Isolating and Identifying One Strain with Lead-Tolerant Fungus and Preliminary Study on Its Capability of Biosorption to Pb<sup>2+</sup> Biology lead Sarocladium SEM XRD FTIR |
| title | Isolating and Identifying One Strain with Lead-Tolerant Fungus and Preliminary Study on Its Capability of Biosorption to Pb<sup>2+</sup> |
| title_full | Isolating and Identifying One Strain with Lead-Tolerant Fungus and Preliminary Study on Its Capability of Biosorption to Pb<sup>2+</sup> |
| title_fullStr | Isolating and Identifying One Strain with Lead-Tolerant Fungus and Preliminary Study on Its Capability of Biosorption to Pb<sup>2+</sup> |
| title_full_unstemmed | Isolating and Identifying One Strain with Lead-Tolerant Fungus and Preliminary Study on Its Capability of Biosorption to Pb<sup>2+</sup> |
| title_short | Isolating and Identifying One Strain with Lead-Tolerant Fungus and Preliminary Study on Its Capability of Biosorption to Pb<sup>2+</sup> |
| title_sort | isolating and identifying one strain with lead tolerant fungus and preliminary study on its capability of biosorption to pb sup 2 sup |
| topic | lead Sarocladium SEM XRD FTIR |
| url | https://www.mdpi.com/2079-7737/13/12/1053 |
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