Effect of LncRNA PTENP1 on TGF-β-induced Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma
Objective To investigate the role of lncRNA PTENP1 in regulating TGF-β-induced epithelial-mesenchymal transition (EMT) in esophageal squamous cell carcinoma (ESCC). Methods Eca109 and TE-1 cells were treated with TGF-β1, and the expression of PTENP1 was detected by qRT-PCR before and after treatment...
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| Format: | Article |
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Magazine House of Cancer Research on Prevention and Treatment
2023-09-01
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| Series: | Zhongliu Fangzhi Yanjiu |
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| Online Access: | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.22.1246 |
| _version_ | 1797671746493480960 |
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| author | FU Shenbo GONG Tuotuo GUO Junjun |
| author_facet | FU Shenbo GONG Tuotuo GUO Junjun |
| author_sort | FU Shenbo |
| collection | DOAJ |
| description | Objective To investigate the role of lncRNA PTENP1 in regulating TGF-β-induced epithelial-mesenchymal transition (EMT) in esophageal squamous cell carcinoma (ESCC). Methods Eca109 and TE-1 cells were treated with TGF-β1, and the expression of PTENP1 was detected by qRT-PCR before and after treatment. PTENP1-overexpressing stably transfected cell lines were constructed in Eca109 and TE-1 cells. The effects of overexpression of PTENP1 on TGF-β1-induced migration, proliferation and EMT-related proteins expression in Eca109 and TE-1 cells were detected by Transwell assay, CCK-8 test and Western blot, respectively. Results The expression of PTENP1 was significantly decreased in Eca109 and TE-1 cells treated with TGF-β1 (P < 0.05). Overexpression of PTENP1 significantly prevented cell migration, decreased the cell vitality, upregulated the E-cadherin expression, and downregulated the expression of N-cadherin and vimentin in Eca109 and TE-1 cells (P < 0.05). Furthermore, PTENP1 overexpression attenuated TGF-β-induced migration of Eca109 and TE-1 cells. PTENP1 overexpression partially reversed TGF-β-induced EMT (P < 0.05). Conclusion PTENP1 plays an important role in TGF-β-induced EMT in ESCC cells. |
| first_indexed | 2024-03-11T21:20:01Z |
| format | Article |
| id | doaj.art-7dcb7dab4fee4d48a572cefa4f737ca8 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 |
| language | zho |
| last_indexed | 2024-03-11T21:20:01Z |
| publishDate | 2023-09-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-7dcb7dab4fee4d48a572cefa4f737ca82023-09-28T08:05:29ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782023-09-0150984785310.3971/j.issn.1000-8578.2023.22.12468578.2023.22.1246Effect of LncRNA PTENP1 on TGF-β-induced Epithelial-mesenchymal Transition in Esophageal Squamous Cell CarcinomaFU Shenbo0GONG Tuotuo1GUO Junjun2Department of Radiation Oncology, Shaanxi Provincial Tumor Hospital, Xi'an 710061, ChinaDepartment of Radiation Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, ChinaDepartment of Radiation Oncology, Shaanxi Provincial Tumor Hospital, Xi'an 710061, ChinaObjective To investigate the role of lncRNA PTENP1 in regulating TGF-β-induced epithelial-mesenchymal transition (EMT) in esophageal squamous cell carcinoma (ESCC). Methods Eca109 and TE-1 cells were treated with TGF-β1, and the expression of PTENP1 was detected by qRT-PCR before and after treatment. PTENP1-overexpressing stably transfected cell lines were constructed in Eca109 and TE-1 cells. The effects of overexpression of PTENP1 on TGF-β1-induced migration, proliferation and EMT-related proteins expression in Eca109 and TE-1 cells were detected by Transwell assay, CCK-8 test and Western blot, respectively. Results The expression of PTENP1 was significantly decreased in Eca109 and TE-1 cells treated with TGF-β1 (P < 0.05). Overexpression of PTENP1 significantly prevented cell migration, decreased the cell vitality, upregulated the E-cadherin expression, and downregulated the expression of N-cadherin and vimentin in Eca109 and TE-1 cells (P < 0.05). Furthermore, PTENP1 overexpression attenuated TGF-β-induced migration of Eca109 and TE-1 cells. PTENP1 overexpression partially reversed TGF-β-induced EMT (P < 0.05). Conclusion PTENP1 plays an important role in TGF-β-induced EMT in ESCC cells.http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.22.1246esophageal squamous cell carcinomalncrna ptenp1tgf-βmigrationepithelial-mesenchymal transition |
| spellingShingle | FU Shenbo GONG Tuotuo GUO Junjun Effect of LncRNA PTENP1 on TGF-β-induced Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma Zhongliu Fangzhi Yanjiu esophageal squamous cell carcinoma lncrna ptenp1 tgf-β migration epithelial-mesenchymal transition |
| title | Effect of LncRNA PTENP1 on TGF-β-induced Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma |
| title_full | Effect of LncRNA PTENP1 on TGF-β-induced Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma |
| title_fullStr | Effect of LncRNA PTENP1 on TGF-β-induced Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma |
| title_full_unstemmed | Effect of LncRNA PTENP1 on TGF-β-induced Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma |
| title_short | Effect of LncRNA PTENP1 on TGF-β-induced Epithelial-mesenchymal Transition in Esophageal Squamous Cell Carcinoma |
| title_sort | effect of lncrna ptenp1 on tgf β induced epithelial mesenchymal transition in esophageal squamous cell carcinoma |
| topic | esophageal squamous cell carcinoma lncrna ptenp1 tgf-β migration epithelial-mesenchymal transition |
| url | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2023.22.1246 |
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