Superoxide and Hydrogen Peroxide Play Different Roles in the Nonhost Interaction of Barley and Wheat with Inappropriate formae speciales of Blumeria graminis
Nonhost resistance of cereals to inappropriate formae speciales of Blumeria graminis is little understood. However, on the microscopic level, nonhost defense to B. graminis is reminiscent of host defense preventing fungal development by penetration resistance and the hypersensitive cell death respon...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2004-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.3.304 |
| _version_ | 1811275710716706816 |
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| author | Marco Trujillo Karl-Heinz Kogel Ralph Hückelhoven |
| author_facet | Marco Trujillo Karl-Heinz Kogel Ralph Hückelhoven |
| author_sort | Marco Trujillo |
| collection | DOAJ |
| description | Nonhost resistance of cereals to inappropriate formae speciales of Blumeria graminis is little understood. However, on the microscopic level, nonhost defense to B. graminis is reminiscent of host defense preventing fungal development by penetration resistance and the hypersensitive cell death response (HR). We analyzed histochemically the accumulation of superoxide anion radicals (O2•¯) and hydrogen peroxide (H2O2) at sites of B. graminis attack in nonhost barley and wheat. Superoxide visualized by subcellular reduction of nitroblue tetrazolium accumulated in association with successful fungal penetration in attacked cells and in cells neighboring HR. In contrast, H2O2 accumulated in cell wall appositions beneath fungal penetration attempts or in the entire epidermal cell during HR. The data provide evidence for different roles and sources of superoxide and H2O2 in the nonhost interaction of cereals with inappropriate formae speciales of B. graminis. |
| first_indexed | 2024-04-12T23:42:38Z |
| format | Article |
| id | doaj.art-7dd021be41a64a44be657a4d16879f71 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T23:42:38Z |
| publishDate | 2004-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-7dd021be41a64a44be657a4d16879f712022-12-22T03:11:56ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062004-03-0117330431210.1094/MPMI.2004.17.3.304Superoxide and Hydrogen Peroxide Play Different Roles in the Nonhost Interaction of Barley and Wheat with Inappropriate formae speciales of Blumeria graminisMarco TrujilloKarl-Heinz KogelRalph HückelhovenNonhost resistance of cereals to inappropriate formae speciales of Blumeria graminis is little understood. However, on the microscopic level, nonhost defense to B. graminis is reminiscent of host defense preventing fungal development by penetration resistance and the hypersensitive cell death response (HR). We analyzed histochemically the accumulation of superoxide anion radicals (O2•¯) and hydrogen peroxide (H2O2) at sites of B. graminis attack in nonhost barley and wheat. Superoxide visualized by subcellular reduction of nitroblue tetrazolium accumulated in association with successful fungal penetration in attacked cells and in cells neighboring HR. In contrast, H2O2 accumulated in cell wall appositions beneath fungal penetration attempts or in the entire epidermal cell during HR. The data provide evidence for different roles and sources of superoxide and H2O2 in the nonhost interaction of cereals with inappropriate formae speciales of B. graminis.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.3.304 |
| spellingShingle | Marco Trujillo Karl-Heinz Kogel Ralph Hückelhoven Superoxide and Hydrogen Peroxide Play Different Roles in the Nonhost Interaction of Barley and Wheat with Inappropriate formae speciales of Blumeria graminis Molecular Plant-Microbe Interactions |
| title | Superoxide and Hydrogen Peroxide Play Different Roles in the Nonhost Interaction of Barley and Wheat with Inappropriate formae speciales of Blumeria graminis |
| title_full | Superoxide and Hydrogen Peroxide Play Different Roles in the Nonhost Interaction of Barley and Wheat with Inappropriate formae speciales of Blumeria graminis |
| title_fullStr | Superoxide and Hydrogen Peroxide Play Different Roles in the Nonhost Interaction of Barley and Wheat with Inappropriate formae speciales of Blumeria graminis |
| title_full_unstemmed | Superoxide and Hydrogen Peroxide Play Different Roles in the Nonhost Interaction of Barley and Wheat with Inappropriate formae speciales of Blumeria graminis |
| title_short | Superoxide and Hydrogen Peroxide Play Different Roles in the Nonhost Interaction of Barley and Wheat with Inappropriate formae speciales of Blumeria graminis |
| title_sort | superoxide and hydrogen peroxide play different roles in the nonhost interaction of barley and wheat with inappropriate formae speciales of blumeria graminis |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.3.304 |
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