Quality uniformity evaluation of Babao Dan capsule based on its 14 main chemical compounds and in vitro anti-inflammatory activity
Babao Dan capsule (BBDC) is a state secrecy traditional Chinese medicine patent prescription (TCMPP) that is widely used for hepatobiliary and urinary diseases. However, its quality uniformity evaluation (QUE) is lacking. It is necessary to develop a novel and multi–dimensional QUE strategy for BBDC...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223007384 |
| _version_ | 1797657890669985792 |
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| author | Anyi Zhao Yanping Liu Lianmei Wang Cong Guo Jun Zhang Sha Chen Qingxia Xu Jintang Cheng Yongzhen Lao Li Che Zhicheng Lai Lihua Yang Yan Liu An Liu |
| author_facet | Anyi Zhao Yanping Liu Lianmei Wang Cong Guo Jun Zhang Sha Chen Qingxia Xu Jintang Cheng Yongzhen Lao Li Che Zhicheng Lai Lihua Yang Yan Liu An Liu |
| author_sort | Anyi Zhao |
| collection | DOAJ |
| description | Babao Dan capsule (BBDC) is a state secrecy traditional Chinese medicine patent prescription (TCMPP) that is widely used for hepatobiliary and urinary diseases. However, its quality uniformity evaluation (QUE) is lacking. It is necessary to develop a novel and multi–dimensional QUE strategy for BBDC based on chemical properties and bioactivities. An ultrahigh performance liquid chromatography–triple quadrupole–mass spectrometry (UHPLC–QQQ–MS/MS) was developed to quantify 14 compounds, and a QUE strategy was to assess intra–batch and inter–batches consistency. Furthermore, the anti-inflammatory activities were measured in vitro by RAW264.7 cell and NF-κB-RE-luc HEK293 cell. The results indicated that 14 compounds from 16 samples (10 batches) were accurately quantified. And the intra–batch consistency values were less than or equal to 20.1%, whereas the largest inter–batches consistency value was 167.5% of glycocholic acid. 10 batches were divided into three categories to distinction inter–batches differences. BBDC had anti-inflammatory activities including inhibition of LPS–induced NO production and TNF α–activated luciferase counts. The NO inhibition difference of 10 batches was 10.87 μM and accounted for 49.8%. Briefly, the anti-inflammatory activities of 10 batches were different, but the dynamic trends within the same batch were similar, which was supported by luciferase counts. And the correlation was confirmed that the main compounds related to anti-inflammatory in BBDC were bile acids and saponins. Hence, a QUE strategy for BBDC was proposed to improve quality and ensure efficacy. This strategy can be utilized as a reference for consistency evaluations of TCMPPs. |
| first_indexed | 2024-03-11T17:51:04Z |
| format | Article |
| id | doaj.art-7dd02305e3e74e1c9501a50bf96bf19a |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-11T17:51:04Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-7dd02305e3e74e1c9501a50bf96bf19a2023-10-18T04:30:48ZengElsevierArabian Journal of Chemistry1878-53522023-11-011611105276Quality uniformity evaluation of Babao Dan capsule based on its 14 main chemical compounds and in vitro anti-inflammatory activityAnyi Zhao0Yanping Liu1Lianmei Wang2Cong Guo3Jun Zhang4Sha Chen5Qingxia Xu6Jintang Cheng7Yongzhen Lao8Li Che9Zhicheng Lai10Lihua Yang11Yan Liu12An Liu13Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR ChinaXiamen Traditional Chinese Medicine company of limited liability, Xiamen 361100, PR ChinaXiamen Traditional Chinese Medicine company of limited liability, Xiamen 361100, PR ChinaXiamen Traditional Chinese Medicine company of limited liability, Xiamen 361100, PR ChinaInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China; Corresponding authors at: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China; Corresponding authors at: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie, Dongzhimennei, Beijing 100700, China.Babao Dan capsule (BBDC) is a state secrecy traditional Chinese medicine patent prescription (TCMPP) that is widely used for hepatobiliary and urinary diseases. However, its quality uniformity evaluation (QUE) is lacking. It is necessary to develop a novel and multi–dimensional QUE strategy for BBDC based on chemical properties and bioactivities. An ultrahigh performance liquid chromatography–triple quadrupole–mass spectrometry (UHPLC–QQQ–MS/MS) was developed to quantify 14 compounds, and a QUE strategy was to assess intra–batch and inter–batches consistency. Furthermore, the anti-inflammatory activities were measured in vitro by RAW264.7 cell and NF-κB-RE-luc HEK293 cell. The results indicated that 14 compounds from 16 samples (10 batches) were accurately quantified. And the intra–batch consistency values were less than or equal to 20.1%, whereas the largest inter–batches consistency value was 167.5% of glycocholic acid. 10 batches were divided into three categories to distinction inter–batches differences. BBDC had anti-inflammatory activities including inhibition of LPS–induced NO production and TNF α–activated luciferase counts. The NO inhibition difference of 10 batches was 10.87 μM and accounted for 49.8%. Briefly, the anti-inflammatory activities of 10 batches were different, but the dynamic trends within the same batch were similar, which was supported by luciferase counts. And the correlation was confirmed that the main compounds related to anti-inflammatory in BBDC were bile acids and saponins. Hence, a QUE strategy for BBDC was proposed to improve quality and ensure efficacy. This strategy can be utilized as a reference for consistency evaluations of TCMPPs.http://www.sciencedirect.com/science/article/pii/S1878535223007384Babao Dan capsuleQuality uniformity evaluationUHPLC–QQQ–MS/MSAnti-inflammatory |
| spellingShingle | Anyi Zhao Yanping Liu Lianmei Wang Cong Guo Jun Zhang Sha Chen Qingxia Xu Jintang Cheng Yongzhen Lao Li Che Zhicheng Lai Lihua Yang Yan Liu An Liu Quality uniformity evaluation of Babao Dan capsule based on its 14 main chemical compounds and in vitro anti-inflammatory activity Arabian Journal of Chemistry Babao Dan capsule Quality uniformity evaluation UHPLC–QQQ–MS/MS Anti-inflammatory |
| title | Quality uniformity evaluation of Babao Dan capsule based on its 14 main chemical compounds and in vitro anti-inflammatory activity |
| title_full | Quality uniformity evaluation of Babao Dan capsule based on its 14 main chemical compounds and in vitro anti-inflammatory activity |
| title_fullStr | Quality uniformity evaluation of Babao Dan capsule based on its 14 main chemical compounds and in vitro anti-inflammatory activity |
| title_full_unstemmed | Quality uniformity evaluation of Babao Dan capsule based on its 14 main chemical compounds and in vitro anti-inflammatory activity |
| title_short | Quality uniformity evaluation of Babao Dan capsule based on its 14 main chemical compounds and in vitro anti-inflammatory activity |
| title_sort | quality uniformity evaluation of babao dan capsule based on its 14 main chemical compounds and in vitro anti inflammatory activity |
| topic | Babao Dan capsule Quality uniformity evaluation UHPLC–QQQ–MS/MS Anti-inflammatory |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223007384 |
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