Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants
Capripox viruses, with their members “lumpy skin disease virus (LSDV)”, “goatpox virus (GTPV)” and “sheeppox virus (SPPV)”, are described as the most serious pox diseases of production animals. A GTPV isolate and a SPPV isolate were sequenced in a combined approach using nanopore MinION sequencing t...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/10/1098 |
| _version_ | 1797552355379511296 |
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| author | Janika Wolff Jacqueline King Tom Moritz Anne Pohlmann Donata Hoffmann Martin Beer Bernd Hoffmann |
| author_facet | Janika Wolff Jacqueline King Tom Moritz Anne Pohlmann Donata Hoffmann Martin Beer Bernd Hoffmann |
| author_sort | Janika Wolff |
| collection | DOAJ |
| description | Capripox viruses, with their members “lumpy skin disease virus (LSDV)”, “goatpox virus (GTPV)” and “sheeppox virus (SPPV)”, are described as the most serious pox diseases of production animals. A GTPV isolate and a SPPV isolate were sequenced in a combined approach using nanopore MinION sequencing to obtain long reads and Illumina high throughput sequencing for short precise reads to gain full-length high-quality genome sequences. Concomitantly, sheep and goats were inoculated with SPPV and GTPV strains, respectively. During the animal trial, varying infection routes were compared: a combined intravenous and subcutaneous infection, an only intranasal infection, and the contact infection between naïve and inoculated animals. Sheep inoculated with SPPV showed no clinical signs, only a very small number of genome-positive samples and a low-level antibody reaction. In contrast, all GTPV inoculated or in-contact goats developed severe clinical signs with high viral genome loads observed in all tested matrices. Furthermore, seroconversion was detected in nearly all goats and no differences concerning the severity of the disease depending on the inoculation route were observed. Conclusively, the employed SPPV strain has the properties of an attenuated vaccine strain, consistent with the genetic data, whereas the GTPV strain represents a highly virulent field strain. |
| first_indexed | 2024-03-10T15:58:53Z |
| format | Article |
| id | doaj.art-7dd04fae1e224c11928498b691a9924e |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T15:58:53Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-7dd04fae1e224c11928498b691a9924e2023-11-20T15:26:09ZengMDPI AGViruses1999-49152020-09-011210109810.3390/v12101098Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small RuminantsJanika Wolff0Jacqueline King1Tom Moritz2Anne Pohlmann3Donata Hoffmann4Martin Beer5Bernd Hoffmann6Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, D-17493 Greifswald-Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, D-17493 Greifswald-Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, D-17493 Greifswald-Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, D-17493 Greifswald-Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, D-17493 Greifswald-Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, D-17493 Greifswald-Insel Riems, GermanyInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, D-17493 Greifswald-Insel Riems, GermanyCapripox viruses, with their members “lumpy skin disease virus (LSDV)”, “goatpox virus (GTPV)” and “sheeppox virus (SPPV)”, are described as the most serious pox diseases of production animals. A GTPV isolate and a SPPV isolate were sequenced in a combined approach using nanopore MinION sequencing to obtain long reads and Illumina high throughput sequencing for short precise reads to gain full-length high-quality genome sequences. Concomitantly, sheep and goats were inoculated with SPPV and GTPV strains, respectively. During the animal trial, varying infection routes were compared: a combined intravenous and subcutaneous infection, an only intranasal infection, and the contact infection between naïve and inoculated animals. Sheep inoculated with SPPV showed no clinical signs, only a very small number of genome-positive samples and a low-level antibody reaction. In contrast, all GTPV inoculated or in-contact goats developed severe clinical signs with high viral genome loads observed in all tested matrices. Furthermore, seroconversion was detected in nearly all goats and no differences concerning the severity of the disease depending on the inoculation route were observed. Conclusively, the employed SPPV strain has the properties of an attenuated vaccine strain, consistent with the genetic data, whereas the GTPV strain represents a highly virulent field strain.https://www.mdpi.com/1999-4915/12/10/1098capripoxsheeppoxgoatpoxSPPVGTPVfield strain |
| spellingShingle | Janika Wolff Jacqueline King Tom Moritz Anne Pohlmann Donata Hoffmann Martin Beer Bernd Hoffmann Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants Viruses capripox sheeppox goatpox SPPV GTPV field strain |
| title | Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants |
| title_full | Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants |
| title_fullStr | Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants |
| title_full_unstemmed | Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants |
| title_short | Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants |
| title_sort | experimental infection and genetic characterization of two different capripox virus isolates in small ruminants |
| topic | capripox sheeppox goatpox SPPV GTPV field strain |
| url | https://www.mdpi.com/1999-4915/12/10/1098 |
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