Investigating the Erα and Cyclin D1 Gene Expression Levels, Changes in SOD, NO, and MDA Levels, and Histopathology of Mice Testicular Tissue Treated with AFG1
Background and purpose: Previous studies have shown the adverse effects of aflatoxin G1 on testicular tissue and the process of spermatogenesis. The aim of this study was to investigate changes in the expression of Cyclin D1, the estrogen receptor ERα and the levels of nitric oxide (NO), superoxide...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences
2021-07-01
|
| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-16319-en.html |
| _version_ | 1828055078911606784 |
|---|---|
| author | Toraj Zamir-nasta Seyran Kakebaraei Mona Pazhouhi Cyrus Jalili |
| author_facet | Toraj Zamir-nasta Seyran Kakebaraei Mona Pazhouhi Cyrus Jalili |
| author_sort | Toraj Zamir-nasta |
| collection | DOAJ |
| description | Background and purpose: Previous studies have shown the adverse effects of aflatoxin G1 on testicular tissue and the process of spermatogenesis. The aim of this study was to investigate changes in the expression of Cyclin D1, the estrogen receptor ERα and the levels of nitric oxide (NO), superoxide dismutase (SOD), and malondialdehyde (MDA) in testicular tissue following exposure to AFG1.
Materials and methods: Twenty four mice were divided into four groups (n=6 per group); a control group (saline) and three treatment groups to receive AFG1 for 7, 15, and 28 days intraperitoneally. Histopathological changes caused by this toxin along with the expression of Cyclin D1 and Erα, as well as Nitric oxid (NO), superoxide dismutase (SOD), and malondialdehyde (MDA) levels in testicular tissue were measured.
Results: The expression of Cyclin D1 in testicular tissue in AFG1 receiving groups increased in a time-dependent manner and the expression of ERα decreased in testicular tissue in AFG1 receiving groups (P<0.05). Also, AFG1 decreased the level of SOD in testicular tissue in a time-dependent manner and increased the levels of MDA and NO. Atrophy of the seminiferous tubules and extensive intercellular edema were seen in treatment groups compared to the control group.
Conclusion: AFG1 disrupts the process of cell division by atrophy of spermatozoa tubules in testicular tissue. It also reduced the expression of ERα receptors and increased the expression of Cyclin D1 along with decreasing the level of SOD in testicular tissue and increased MDA and NO levels. These factors can cause disorders in the process of spermatogenesis. |
| first_indexed | 2024-04-10T20:32:58Z |
| format | Article |
| id | doaj.art-7dd4ac8fcb37491f858fea3bdfc91f78 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:32:58Z |
| publishDate | 2021-07-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-7dd4ac8fcb37491f858fea3bdfc91f782023-01-25T05:14:26ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792021-07-0131198112Investigating the Erα and Cyclin D1 Gene Expression Levels, Changes in SOD, NO, and MDA Levels, and Histopathology of Mice Testicular Tissue Treated with AFG1Toraj Zamir-nasta0Seyran Kakebaraei1Mona Pazhouhi2Cyrus Jalili3 PhD Student in Phatology, Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran PhD Student in Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran PhD in Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Professor, Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Background and purpose: Previous studies have shown the adverse effects of aflatoxin G1 on testicular tissue and the process of spermatogenesis. The aim of this study was to investigate changes in the expression of Cyclin D1, the estrogen receptor ERα and the levels of nitric oxide (NO), superoxide dismutase (SOD), and malondialdehyde (MDA) in testicular tissue following exposure to AFG1. Materials and methods: Twenty four mice were divided into four groups (n=6 per group); a control group (saline) and three treatment groups to receive AFG1 for 7, 15, and 28 days intraperitoneally. Histopathological changes caused by this toxin along with the expression of Cyclin D1 and Erα, as well as Nitric oxid (NO), superoxide dismutase (SOD), and malondialdehyde (MDA) levels in testicular tissue were measured. Results: The expression of Cyclin D1 in testicular tissue in AFG1 receiving groups increased in a time-dependent manner and the expression of ERα decreased in testicular tissue in AFG1 receiving groups (P<0.05). Also, AFG1 decreased the level of SOD in testicular tissue in a time-dependent manner and increased the levels of MDA and NO. Atrophy of the seminiferous tubules and extensive intercellular edema were seen in treatment groups compared to the control group. Conclusion: AFG1 disrupts the process of cell division by atrophy of spermatozoa tubules in testicular tissue. It also reduced the expression of ERα receptors and increased the expression of Cyclin D1 along with decreasing the level of SOD in testicular tissue and increased MDA and NO levels. These factors can cause disorders in the process of spermatogenesis.http://jmums.mazums.ac.ir/article-1-16319-en.htmlaflatoxin g1spermatogenesiserαcyclin d1oxidative stressmice |
| spellingShingle | Toraj Zamir-nasta Seyran Kakebaraei Mona Pazhouhi Cyrus Jalili Investigating the Erα and Cyclin D1 Gene Expression Levels, Changes in SOD, NO, and MDA Levels, and Histopathology of Mice Testicular Tissue Treated with AFG1 Journal of Mazandaran University of Medical Sciences aflatoxin g1 spermatogenesis erα cyclin d1 oxidative stress mice |
| title | Investigating the Erα and Cyclin D1 Gene Expression Levels, Changes in SOD, NO, and MDA Levels, and Histopathology of Mice Testicular Tissue Treated with AFG1 |
| title_full | Investigating the Erα and Cyclin D1 Gene Expression Levels, Changes in SOD, NO, and MDA Levels, and Histopathology of Mice Testicular Tissue Treated with AFG1 |
| title_fullStr | Investigating the Erα and Cyclin D1 Gene Expression Levels, Changes in SOD, NO, and MDA Levels, and Histopathology of Mice Testicular Tissue Treated with AFG1 |
| title_full_unstemmed | Investigating the Erα and Cyclin D1 Gene Expression Levels, Changes in SOD, NO, and MDA Levels, and Histopathology of Mice Testicular Tissue Treated with AFG1 |
| title_short | Investigating the Erα and Cyclin D1 Gene Expression Levels, Changes in SOD, NO, and MDA Levels, and Histopathology of Mice Testicular Tissue Treated with AFG1 |
| title_sort | investigating the erα and cyclin d1 gene expression levels changes in sod no and mda levels and histopathology of mice testicular tissue treated with afg1 |
| topic | aflatoxin g1 spermatogenesis erα cyclin d1 oxidative stress mice |
| url | http://jmums.mazums.ac.ir/article-1-16319-en.html |
| work_keys_str_mv | AT torajzamirnasta investigatingtheeraandcyclind1geneexpressionlevelschangesinsodnoandmdalevelsandhistopathologyofmicetesticulartissuetreatedwithafg1 AT seyrankakebaraei investigatingtheeraandcyclind1geneexpressionlevelschangesinsodnoandmdalevelsandhistopathologyofmicetesticulartissuetreatedwithafg1 AT monapazhouhi investigatingtheeraandcyclind1geneexpressionlevelschangesinsodnoandmdalevelsandhistopathologyofmicetesticulartissuetreatedwithafg1 AT cyrusjalili investigatingtheeraandcyclind1geneexpressionlevelschangesinsodnoandmdalevelsandhistopathologyofmicetesticulartissuetreatedwithafg1 |