Toxins profiles, toxicological properties, and histological alteration potentiality of Trimeresurus erythrurus venom: In vitro and in vivo experiments
Objectives: Trimeresurus erythrurus (Red-tailed bamboo pit viper) contributes significantly to snakebite envenoming cases in Bangladesh, India, Myanmar, and Nepal. However, the absence of specific antivenom and challenging socio-economic conditions in these regions necessitate the development of sym...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364724000624 |
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| author | Ibrahim Khalil Al Haidar Mohammad Abdul Wahed Chowdhury Masum Miah Mahedi Hasan Md Sohanur Rohman Sohan Mohammed Noman Md. Mizanur Rahman Abdul Auawal Sajib Rudra Md. Rafiqul Islam Md. Asir Uddin Abdullah Abu Sayeed Aniruddha Ghose M. Monirul Islam Md Abu Reza |
| author_facet | Ibrahim Khalil Al Haidar Mohammad Abdul Wahed Chowdhury Masum Miah Mahedi Hasan Md Sohanur Rohman Sohan Mohammed Noman Md. Mizanur Rahman Abdul Auawal Sajib Rudra Md. Rafiqul Islam Md. Asir Uddin Abdullah Abu Sayeed Aniruddha Ghose M. Monirul Islam Md Abu Reza |
| author_sort | Ibrahim Khalil Al Haidar |
| collection | DOAJ |
| description | Objectives: Trimeresurus erythrurus (Red-tailed bamboo pit viper) contributes significantly to snakebite envenoming cases in Bangladesh, India, Myanmar, and Nepal. However, the absence of specific antivenom and challenging socio-economic conditions in these regions necessitate the development of symptom-based medication to mitigate the severity of T. erythrurus envenoming. Therefore, documenting the effects of T. erythrurus venom on mammalian organs can establish a foundation for further research on symptom-based medication. Methods: We profiled T. erythrurus venom toxins using SDS-PAGE. Additionally, we assessed the venom's toxicological properties through lethality, hemorrhagic, edematogenic, hemolytic, and phospholytic assays. Furthermore, we examined the histological alterations induced by crude venom on various organs of mice. Results: SDS-PAGE profiling confirmed the presence of Snake Venom Metalloproteases (SVMPs), Snake Venom Serine Proteases (SVSPs), Phospholipase A2 (PLA2), L-amino Acid Oxidases (LAAOs), Myotoxins, and Disintegrins as toxic components in T. erythrurus venom. The venom exhibited a Median Lethal Dose (LD50) of 1.131 mg/kg, Minimum Hemorrhagic Dose (MHD) of 0.21 mg/kg, Minimum Edematogenic Dose (MED) of 0.17 mg/kg, and Median Hemolytic Dose (HD50) of 2.527 mg/L. Moreover, the venom induced significant pathological changes in mammalian skin, skeletal muscle, lung, heart, kidney, and intestine. Conclusions: This study elucidates the major toxins, critical toxicological indices, and histopathological effects of T. erythrurus venom on mammalian organs. These findings provide valuable insights on the development of effective treatment strategies for pathophysiological complications that could manifest post T. erythrurus envenoming. |
| first_indexed | 2024-04-24T10:05:21Z |
| format | Article |
| id | doaj.art-7dd5e4ec5a9142948f383d3a64204721 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-24T10:05:21Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-7dd5e4ec5a9142948f383d3a642047212024-04-13T04:20:58ZengElsevierJournal of King Saud University: Science1018-36472024-05-01365103150Toxins profiles, toxicological properties, and histological alteration potentiality of Trimeresurus erythrurus venom: In vitro and in vivo experimentsIbrahim Khalil Al Haidar0Mohammad Abdul Wahed Chowdhury1Masum Miah2Mahedi Hasan3Md Sohanur Rohman Sohan4Mohammed Noman5Md. Mizanur Rahman6Abdul Auawal7Sajib Rudra8Md. Rafiqul Islam9Md. Asir Uddin10Abdullah Abu Sayeed11Aniruddha Ghose12M. Monirul Islam13Md Abu Reza14Department of Zoology, University of Chittagong, Chattogram 4331, Bangladesh; Venom Research Centre, Chittagong Medical College, Chattogram 4203, Bangladesh; Department of Biochemistry and Molecular Biology, University of Chittagong, Chattogram 4331, Bangladesh; Corresponding authors at: Department of Zoology, University of Chittagong, Chattogram 4331, Bangladesh (IKA Haidar, MAW Chowdhury) and Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh (MA Reza).Department of Zoology, University of Chittagong, Chattogram 4331, Bangladesh; Venom Research Centre, Chittagong Medical College, Chattogram 4203, Bangladesh; Corresponding authors at: Department of Zoology, University of Chittagong, Chattogram 4331, Bangladesh (IKA Haidar, MAW Chowdhury) and Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh (MA Reza).Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMolecular Biology and Protein Science Laboratory, Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMolecular Biology and Protein Science Laboratory, Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshVenom Research Centre, Chittagong Medical College, Chattogram 4203, BangladeshVenom Research Centre, Chittagong Medical College, Chattogram 4203, BangladeshVenom Research Centre, Chittagong Medical College, Chattogram 4203, BangladeshVenom Research Centre, Chittagong Medical College, Chattogram 4203, BangladeshDepartment of Zoology, University of Chittagong, Chattogram 4331, Bangladesh; Venom Research Centre, Chittagong Medical College, Chattogram 4203, BangladeshDepartment of Zoology, University of Chittagong, Chattogram 4331, Bangladesh; Venom Research Centre, Chittagong Medical College, Chattogram 4203, BangladeshVenom Research Centre, Chittagong Medical College, Chattogram 4203, Bangladesh; Department of Medicine, Chittagong Medical College, Chattogram 4203, BangladeshVenom Research Centre, Chittagong Medical College, Chattogram 4203, Bangladesh; Department of Medicine, Chittagong Medical College, Chattogram 4203, BangladeshDepartment of Biochemistry and Molecular Biology, University of Chittagong, Chattogram 4331, BangladeshMolecular Biology and Protein Science Laboratory, Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh; Corresponding authors at: Department of Zoology, University of Chittagong, Chattogram 4331, Bangladesh (IKA Haidar, MAW Chowdhury) and Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh (MA Reza).Objectives: Trimeresurus erythrurus (Red-tailed bamboo pit viper) contributes significantly to snakebite envenoming cases in Bangladesh, India, Myanmar, and Nepal. However, the absence of specific antivenom and challenging socio-economic conditions in these regions necessitate the development of symptom-based medication to mitigate the severity of T. erythrurus envenoming. Therefore, documenting the effects of T. erythrurus venom on mammalian organs can establish a foundation for further research on symptom-based medication. Methods: We profiled T. erythrurus venom toxins using SDS-PAGE. Additionally, we assessed the venom's toxicological properties through lethality, hemorrhagic, edematogenic, hemolytic, and phospholytic assays. Furthermore, we examined the histological alterations induced by crude venom on various organs of mice. Results: SDS-PAGE profiling confirmed the presence of Snake Venom Metalloproteases (SVMPs), Snake Venom Serine Proteases (SVSPs), Phospholipase A2 (PLA2), L-amino Acid Oxidases (LAAOs), Myotoxins, and Disintegrins as toxic components in T. erythrurus venom. The venom exhibited a Median Lethal Dose (LD50) of 1.131 mg/kg, Minimum Hemorrhagic Dose (MHD) of 0.21 mg/kg, Minimum Edematogenic Dose (MED) of 0.17 mg/kg, and Median Hemolytic Dose (HD50) of 2.527 mg/L. Moreover, the venom induced significant pathological changes in mammalian skin, skeletal muscle, lung, heart, kidney, and intestine. Conclusions: This study elucidates the major toxins, critical toxicological indices, and histopathological effects of T. erythrurus venom on mammalian organs. These findings provide valuable insights on the development of effective treatment strategies for pathophysiological complications that could manifest post T. erythrurus envenoming.http://www.sciencedirect.com/science/article/pii/S1018364724000624Red-tailed bamboo pit viperSnake venomVenom profileVenom toxicitySnakebite management |
| spellingShingle | Ibrahim Khalil Al Haidar Mohammad Abdul Wahed Chowdhury Masum Miah Mahedi Hasan Md Sohanur Rohman Sohan Mohammed Noman Md. Mizanur Rahman Abdul Auawal Sajib Rudra Md. Rafiqul Islam Md. Asir Uddin Abdullah Abu Sayeed Aniruddha Ghose M. Monirul Islam Md Abu Reza Toxins profiles, toxicological properties, and histological alteration potentiality of Trimeresurus erythrurus venom: In vitro and in vivo experiments Journal of King Saud University: Science Red-tailed bamboo pit viper Snake venom Venom profile Venom toxicity Snakebite management |
| title | Toxins profiles, toxicological properties, and histological alteration potentiality of Trimeresurus erythrurus venom: In vitro and in vivo experiments |
| title_full | Toxins profiles, toxicological properties, and histological alteration potentiality of Trimeresurus erythrurus venom: In vitro and in vivo experiments |
| title_fullStr | Toxins profiles, toxicological properties, and histological alteration potentiality of Trimeresurus erythrurus venom: In vitro and in vivo experiments |
| title_full_unstemmed | Toxins profiles, toxicological properties, and histological alteration potentiality of Trimeresurus erythrurus venom: In vitro and in vivo experiments |
| title_short | Toxins profiles, toxicological properties, and histological alteration potentiality of Trimeresurus erythrurus venom: In vitro and in vivo experiments |
| title_sort | toxins profiles toxicological properties and histological alteration potentiality of trimeresurus erythrurus venom in vitro and in vivo experiments |
| topic | Red-tailed bamboo pit viper Snake venom Venom profile Venom toxicity Snakebite management |
| url | http://www.sciencedirect.com/science/article/pii/S1018364724000624 |
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