Protective Effects of Armillaria mellea Polysaccharides on Nicotine-induced Lung Injury in Rats
Objective: To investigate the protective effects of Armillaria mellea polysaccharides on lung injury induced by nicotine in rats. Methods: SD rats were randomly divided into control group, model group and Armillaria mellea polysaccharides low and high dose groups. During modeling, except for the nor...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-08-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100193 |
| _version_ | 1811307827146260480 |
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| author | Ruimeng ZHANG Xin SU Yu LI Xiaoman BAO Minghua SHEN |
| author_facet | Ruimeng ZHANG Xin SU Yu LI Xiaoman BAO Minghua SHEN |
| author_sort | Ruimeng ZHANG |
| collection | DOAJ |
| description | Objective: To investigate the protective effects of Armillaria mellea polysaccharides on lung injury induced by nicotine in rats. Methods: SD rats were randomly divided into control group, model group and Armillaria mellea polysaccharides low and high dose groups. During modeling, except for the normal group, the other groups were intraperitoneally injected with nicotine 2 mg/kg body weight, and the low and high dose groups were gavaged with Armillaria mellea polysaccharides 200 and 400 mg/kg body weight, respectively. The morphologic changes of lung tissue were observed by HE staining. The levels of TNF-α, IL-6 and IL-1β were detected by ELISA, MDA level was detected by TBA method, SOD activity was detected by WST-1 method. The protein expression levels of Nrf2, HO-1 and p-NF-κB in lung tissues were detected by Western blot assay. Results: Compared with the control group, after intervention of nicotine, the levels of TNF-α, IL-6, IL-1β and MDA were increased, the activity of SOD was decreased in plasma, the phosphorylation expression of NF-κB protein was increased, Nrf2 and HO-1 protein expression were decreased in lung tissue. Compared with the model group, after intervention of Armillaria mellea polysaccharides, the degree of lung tissue injury was alleviated, the levels of TNF-α, IL-6, IL-1β and MDA were significantly decreased, the activity of SOD was increased in plasma, the phosphorylation expression of NF-κB protein was significantly decreased, Nrf2 and HO-1 protein expression were significantly increased in lung tissue. Conclusion: Armillaria mellea polysaccharides could inhibit the lung tissue injury by nicotine induced. The mechanism of action may be related to its regulation of NF-κB and Nrf2/HO-1 signaling pathway. |
| first_indexed | 2024-04-13T09:11:24Z |
| format | Article |
| id | doaj.art-7dd8b71f456c423ba42b22cf3eef7eb4 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:11:24Z |
| publishDate | 2022-08-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-7dd8b71f456c423ba42b22cf3eef7eb42022-12-22T02:52:52ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-08-01431637137610.13386/j.issn1002-0306.20211001932021100193-16Protective Effects of Armillaria mellea Polysaccharides on Nicotine-induced Lung Injury in RatsRuimeng ZHANG0Xin SU1Yu LI2Xiaoman BAO3Minghua SHEN4Yanbian University, Yanji 133002, ChinaYanbian University, Yanji 133002, ChinaYanbian University, Yanji 133002, ChinaYanbian University, Yanji 133002, ChinaYanbian University, Yanji 133002, ChinaObjective: To investigate the protective effects of Armillaria mellea polysaccharides on lung injury induced by nicotine in rats. Methods: SD rats were randomly divided into control group, model group and Armillaria mellea polysaccharides low and high dose groups. During modeling, except for the normal group, the other groups were intraperitoneally injected with nicotine 2 mg/kg body weight, and the low and high dose groups were gavaged with Armillaria mellea polysaccharides 200 and 400 mg/kg body weight, respectively. The morphologic changes of lung tissue were observed by HE staining. The levels of TNF-α, IL-6 and IL-1β were detected by ELISA, MDA level was detected by TBA method, SOD activity was detected by WST-1 method. The protein expression levels of Nrf2, HO-1 and p-NF-κB in lung tissues were detected by Western blot assay. Results: Compared with the control group, after intervention of nicotine, the levels of TNF-α, IL-6, IL-1β and MDA were increased, the activity of SOD was decreased in plasma, the phosphorylation expression of NF-κB protein was increased, Nrf2 and HO-1 protein expression were decreased in lung tissue. Compared with the model group, after intervention of Armillaria mellea polysaccharides, the degree of lung tissue injury was alleviated, the levels of TNF-α, IL-6, IL-1β and MDA were significantly decreased, the activity of SOD was increased in plasma, the phosphorylation expression of NF-κB protein was significantly decreased, Nrf2 and HO-1 protein expression were significantly increased in lung tissue. Conclusion: Armillaria mellea polysaccharides could inhibit the lung tissue injury by nicotine induced. The mechanism of action may be related to its regulation of NF-κB and Nrf2/HO-1 signaling pathway.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100193armillaria mellea polysaccharidesnicotinelung injuryinflammationoxidative stress |
| spellingShingle | Ruimeng ZHANG Xin SU Yu LI Xiaoman BAO Minghua SHEN Protective Effects of Armillaria mellea Polysaccharides on Nicotine-induced Lung Injury in Rats Shipin gongye ke-ji armillaria mellea polysaccharides nicotine lung injury inflammation oxidative stress |
| title | Protective Effects of Armillaria mellea Polysaccharides on Nicotine-induced Lung Injury in Rats |
| title_full | Protective Effects of Armillaria mellea Polysaccharides on Nicotine-induced Lung Injury in Rats |
| title_fullStr | Protective Effects of Armillaria mellea Polysaccharides on Nicotine-induced Lung Injury in Rats |
| title_full_unstemmed | Protective Effects of Armillaria mellea Polysaccharides on Nicotine-induced Lung Injury in Rats |
| title_short | Protective Effects of Armillaria mellea Polysaccharides on Nicotine-induced Lung Injury in Rats |
| title_sort | protective effects of armillaria mellea polysaccharides on nicotine induced lung injury in rats |
| topic | armillaria mellea polysaccharides nicotine lung injury inflammation oxidative stress |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100193 |
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