The analysis of coffee-green tea-turmeric combination against cardiac-metabolic syndrome using metabolite profiling, gene expression, and in silico approach
Context: The development of functional drinks to inhibit oxidative stress and inflammation as a critical process in inducing heart damage in metabolic syndrome is required. Coffee, tea, and turmeric have all been shown to offer health advantages. Aims: To investigate the effect of coffee, green t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
GarVal Editorial Ltda.
2023-11-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol11/jppres23.1702_11.6.961.pdf |
| _version_ | 1797363883961221120 |
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| author | Ermin Rachmawati Mohammad S. Rohman Nashi Widodo Mifetika Lukitasari Dwi A. Nugroho Feri E. Hermanto Mukhamad N. Kholis |
| author_facet | Ermin Rachmawati Mohammad S. Rohman Nashi Widodo Mifetika Lukitasari Dwi A. Nugroho Feri E. Hermanto Mukhamad N. Kholis |
| author_sort | Ermin Rachmawati |
| collection | DOAJ |
| description | Context: The development of functional drinks to inhibit oxidative stress and inflammation as a critical process in inducing heart damage in metabolic syndrome is required. Coffee, tea, and turmeric have all been shown to offer health advantages.
Aims: To investigate the effect of coffee, green tea, turmeric extract (ECGTT) against cardiac-metabolic syndrome (MetS).
Methods: The secondary metabolites from coffee, green tea, and turmeric were identified using LC-HRMS. Male Sprague–Dawley rats were divided into four groups (n = 4) representing normal, MetS, MetS with ECGTT treatment doses: 300/100/150 mg/BW and 300/100/250 mg/BW group. Upon the end of treatment periods, expression of tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6), nuclear factor kappa B (NF-κB), NADPH oxidase (NOX2), SERCA2a were measured from the heart. A computational approach including network pharmacology, protein-protein interaction (PPI) network, molecular docking, and dynamic was performed to understand the molecular mechanism of ECGTT against cardiac damage in MetS.
Results: Chlorogenic acid (CGA), epigallocatechin gallate (EGCG), and curcumin were identified as the main metabolites in ECGTT. The ECGTT administration decreased the TNFα, IL-6, NF-κB, and NOX2 and increased SERCA2a expression(p<0.05). Moreover, the PPI result suggested that angiotensin II receptor type 1 (AGTR1) was the key regulator of cardiac injury-MetS induced. CGA, EGCG, and curcumin bind to AGTR1 with smaller binding energy than metformin and showed stability of structure and interaction among those metabolites into AGTR1.
Conclusions: Coffee, green tea, and turmeric might prevent heart dysfunction in MetS through modulation of oxidative stress and inflammation. |
| first_indexed | 2024-03-08T16:27:22Z |
| format | Article |
| id | doaj.art-7dda727b86ef47a4986526812960f08e |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-03-08T16:27:22Z |
| publishDate | 2023-11-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-7dda727b86ef47a4986526812960f08e2024-01-06T16:42:11ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502023-11-0111696197410.56499/jppres23.1702_11.6.961The analysis of coffee-green tea-turmeric combination against cardiac-metabolic syndrome using metabolite profiling, gene expression, and in silico approachErmin Rachmawati0Mohammad S. Rohman1Nashi Widodo2Mifetika Lukitasari3Dwi A. Nugroho4Feri E. Hermanto5Mukhamad N. Kholis6Research Center for Cardiovascular, Brawijaya University, Malang, Indonesia. Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, UIN Maulana Malik Ibrahim Malang, East Java, Indonesia.Research Center for Cardiovascular, Brawijaya University, Malang, Indonesia. Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.Research Center for Cardiovascular, Brawijaya University, Malang, Indonesia. Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, Indonesia.Research Center for Cardiovascular, Brawijaya University, Malang, Indonesia. School of Nursing, Faculty of Health Sciences, Universitas Brawijaya, Malang, Indonesia.Research Center for Cardiovascular, Brawijaya University, Malang, Indonesia.Research Center for Cardiovascular, Brawijaya University, Malang, Indonesia. Faculty of Animal Sciences, Universitas Brawijaya, Malang, Indonesia. Bioinformatics Research Center, Indonesian Institute of Bioinformatics, Malang, Indonesia.Research Center for Cardiovascular, Brawijaya University, Malang, Indonesia. Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, UIN Maulana Malik Ibrahim Malang, East Java, Indonesia.Context: The development of functional drinks to inhibit oxidative stress and inflammation as a critical process in inducing heart damage in metabolic syndrome is required. Coffee, tea, and turmeric have all been shown to offer health advantages. Aims: To investigate the effect of coffee, green tea, turmeric extract (ECGTT) against cardiac-metabolic syndrome (MetS). Methods: The secondary metabolites from coffee, green tea, and turmeric were identified using LC-HRMS. Male Sprague–Dawley rats were divided into four groups (n = 4) representing normal, MetS, MetS with ECGTT treatment doses: 300/100/150 mg/BW and 300/100/250 mg/BW group. Upon the end of treatment periods, expression of tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6), nuclear factor kappa B (NF-κB), NADPH oxidase (NOX2), SERCA2a were measured from the heart. A computational approach including network pharmacology, protein-protein interaction (PPI) network, molecular docking, and dynamic was performed to understand the molecular mechanism of ECGTT against cardiac damage in MetS. Results: Chlorogenic acid (CGA), epigallocatechin gallate (EGCG), and curcumin were identified as the main metabolites in ECGTT. The ECGTT administration decreased the TNFα, IL-6, NF-κB, and NOX2 and increased SERCA2a expression(p<0.05). Moreover, the PPI result suggested that angiotensin II receptor type 1 (AGTR1) was the key regulator of cardiac injury-MetS induced. CGA, EGCG, and curcumin bind to AGTR1 with smaller binding energy than metformin and showed stability of structure and interaction among those metabolites into AGTR1. Conclusions: Coffee, green tea, and turmeric might prevent heart dysfunction in MetS through modulation of oxidative stress and inflammation.https://jppres.com/jppres/pdf/vol11/jppres23.1702_11.6.961.pdfcalcium handlingcoffeegreen teainflammationoxidative stressturmeric |
| spellingShingle | Ermin Rachmawati Mohammad S. Rohman Nashi Widodo Mifetika Lukitasari Dwi A. Nugroho Feri E. Hermanto Mukhamad N. Kholis The analysis of coffee-green tea-turmeric combination against cardiac-metabolic syndrome using metabolite profiling, gene expression, and in silico approach Journal of Pharmacy & Pharmacognosy Research calcium handling coffee green tea inflammation oxidative stress turmeric |
| title | The analysis of coffee-green tea-turmeric combination against cardiac-metabolic syndrome using metabolite profiling, gene expression, and in silico approach |
| title_full | The analysis of coffee-green tea-turmeric combination against cardiac-metabolic syndrome using metabolite profiling, gene expression, and in silico approach |
| title_fullStr | The analysis of coffee-green tea-turmeric combination against cardiac-metabolic syndrome using metabolite profiling, gene expression, and in silico approach |
| title_full_unstemmed | The analysis of coffee-green tea-turmeric combination against cardiac-metabolic syndrome using metabolite profiling, gene expression, and in silico approach |
| title_short | The analysis of coffee-green tea-turmeric combination against cardiac-metabolic syndrome using metabolite profiling, gene expression, and in silico approach |
| title_sort | analysis of coffee green tea turmeric combination against cardiac metabolic syndrome using metabolite profiling gene expression and in silico approach |
| topic | calcium handling coffee green tea inflammation oxidative stress turmeric |
| url | https://jppres.com/jppres/pdf/vol11/jppres23.1702_11.6.961.pdf |
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