Metagenomic Analysis of Bacterial and Fungal Communities Inhabiting Shiro Dominant Soils of Two Production Regions of <i>Tricholoma Matsutake</i> S. Ito & S. Imai in Korea
<i>Tricholoma matsutake</i> is an ectomycorrhizal fungus that has obligate symbiotic relationships with <i>Pinus densiflora</i>. Its fruiting body has a distinctive flavor and is traded at a high price. Thus, it has been a significant source of income for rural communities in...
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MDPI AG
2021-06-01
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| author | Gi-Hong An Jae-Han Cho Ok-Tae Kim Jae-Gu Han |
| author_facet | Gi-Hong An Jae-Han Cho Ok-Tae Kim Jae-Gu Han |
| author_sort | Gi-Hong An |
| collection | DOAJ |
| description | <i>Tricholoma matsutake</i> is an ectomycorrhizal fungus that has obligate symbiotic relationships with <i>Pinus densiflora</i>. Its fruiting body has a distinctive flavor and is traded at a high price. Thus, it has been a significant source of income for rural communities in Korea. We hypothesized that biotic factors considerably influence the formation of the <i>T. matsutake</i> mushroom, and the soils producing <i>T. matsutake</i> share similar microbial characteristics. Therefore, the present study aimed to detect the specific fungal and bacterial groups in <i>T. matsutake</i> production soils (shiro+) and nonproduction soils (shiro−) of the Bonghwa and Yanyang regions via next-generation sequencing. In a total of 15 phyla, 36 classes, 234 genera of bacteria, six phyla, 29 classes, and 164 genera of fungi were detected from four samples at both sites. The species diversity of shiro+ soils was lower than the shiro− samples in both the fungal and bacterial groups. In addition, we did not find high similarities in the microbial communities between the shiro+ soils of the two regions. However, in the resulting differences between the fungal communities categorized by their trophic assembly, we found a distinguishable compositional pattern in the fungal communities from the shiro+ soils and the shiro− soils of the two sites. Thus, the similarity among the microbial communities in the forest soils may be due to the fact that the microbial communities in the <i>T. matsutake</i> dominant soils are closely associated with biotic factors and abiotic factors such as soil properties. |
| first_indexed | 2024-03-10T10:35:14Z |
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| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T10:35:14Z |
| publishDate | 2021-06-01 |
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| series | Forests |
| spelling | doaj.art-7ddd397c24d1447a9de8c1002480705e2023-11-21T23:20:54ZengMDPI AGForests1999-49072021-06-0112675810.3390/f12060758Metagenomic Analysis of Bacterial and Fungal Communities Inhabiting Shiro Dominant Soils of Two Production Regions of <i>Tricholoma Matsutake</i> S. Ito & S. Imai in KoreaGi-Hong An0Jae-Han Cho1Ok-Tae Kim2Jae-Gu Han3Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk 27709, KoreaMushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk 27709, KoreaMushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk 27709, KoreaMushroom Research Division, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk 27709, Korea<i>Tricholoma matsutake</i> is an ectomycorrhizal fungus that has obligate symbiotic relationships with <i>Pinus densiflora</i>. Its fruiting body has a distinctive flavor and is traded at a high price. Thus, it has been a significant source of income for rural communities in Korea. We hypothesized that biotic factors considerably influence the formation of the <i>T. matsutake</i> mushroom, and the soils producing <i>T. matsutake</i> share similar microbial characteristics. Therefore, the present study aimed to detect the specific fungal and bacterial groups in <i>T. matsutake</i> production soils (shiro+) and nonproduction soils (shiro−) of the Bonghwa and Yanyang regions via next-generation sequencing. In a total of 15 phyla, 36 classes, 234 genera of bacteria, six phyla, 29 classes, and 164 genera of fungi were detected from four samples at both sites. The species diversity of shiro+ soils was lower than the shiro− samples in both the fungal and bacterial groups. In addition, we did not find high similarities in the microbial communities between the shiro+ soils of the two regions. However, in the resulting differences between the fungal communities categorized by their trophic assembly, we found a distinguishable compositional pattern in the fungal communities from the shiro+ soils and the shiro− soils of the two sites. Thus, the similarity among the microbial communities in the forest soils may be due to the fact that the microbial communities in the <i>T. matsutake</i> dominant soils are closely associated with biotic factors and abiotic factors such as soil properties.https://www.mdpi.com/1999-4907/12/6/758ectomycorrhizaebacterial communitiesfungal communitiesmetagenomicsmiseqshiro dominant soil |
| spellingShingle | Gi-Hong An Jae-Han Cho Ok-Tae Kim Jae-Gu Han Metagenomic Analysis of Bacterial and Fungal Communities Inhabiting Shiro Dominant Soils of Two Production Regions of <i>Tricholoma Matsutake</i> S. Ito & S. Imai in Korea Forests ectomycorrhizae bacterial communities fungal communities metagenomics miseq shiro dominant soil |
| title | Metagenomic Analysis of Bacterial and Fungal Communities Inhabiting Shiro Dominant Soils of Two Production Regions of <i>Tricholoma Matsutake</i> S. Ito & S. Imai in Korea |
| title_full | Metagenomic Analysis of Bacterial and Fungal Communities Inhabiting Shiro Dominant Soils of Two Production Regions of <i>Tricholoma Matsutake</i> S. Ito & S. Imai in Korea |
| title_fullStr | Metagenomic Analysis of Bacterial and Fungal Communities Inhabiting Shiro Dominant Soils of Two Production Regions of <i>Tricholoma Matsutake</i> S. Ito & S. Imai in Korea |
| title_full_unstemmed | Metagenomic Analysis of Bacterial and Fungal Communities Inhabiting Shiro Dominant Soils of Two Production Regions of <i>Tricholoma Matsutake</i> S. Ito & S. Imai in Korea |
| title_short | Metagenomic Analysis of Bacterial and Fungal Communities Inhabiting Shiro Dominant Soils of Two Production Regions of <i>Tricholoma Matsutake</i> S. Ito & S. Imai in Korea |
| title_sort | metagenomic analysis of bacterial and fungal communities inhabiting shiro dominant soils of two production regions of i tricholoma matsutake i s ito s imai in korea |
| topic | ectomycorrhizae bacterial communities fungal communities metagenomics miseq shiro dominant soil |
| url | https://www.mdpi.com/1999-4907/12/6/758 |
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