Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum
Abstract Background Prolamins, unique to Gramineae (grasses), play a key role in the human diet. Thinopyrum elongatum (syn. Agropyron elongatum or Lophopyrum elongatum), a grass of the Triticeae family with a diploid E genome (2n = 2x = 14), is genetically well-characterized, but little is known abo...
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-08088-x |
| _version_ | 1818721997061881856 |
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| author | Wenyang Ge Yu Gao Shoushen Xu Xin Ma Hongwei Wang Lingrang Kong Silong Sun |
| author_facet | Wenyang Ge Yu Gao Shoushen Xu Xin Ma Hongwei Wang Lingrang Kong Silong Sun |
| author_sort | Wenyang Ge |
| collection | DOAJ |
| description | Abstract Background Prolamins, unique to Gramineae (grasses), play a key role in the human diet. Thinopyrum elongatum (syn. Agropyron elongatum or Lophopyrum elongatum), a grass of the Triticeae family with a diploid E genome (2n = 2x = 14), is genetically well-characterized, but little is known about its prolamin genes and the relationships with homologous loci in the Triticeae species. Results In this study, a total of 19 α-gliadin, 9 γ-gliadin, 19 ω-gliadin, 2 high-molecular-weight glutenin subunit (HMW-GS), and 5 low-molecular-weight glutenin subunit (LMW-GS) genes were identified in the Th. elongatum genome. Micro-synteny and phylogenetic analysis revealed dynamic changes of prolamin gene regions and genetic affinities among Th. elongatum, Triticum aestivum, T. urartu and Aegilops tauschii. The Th. elongatum genome, like the B subgenome of T. aestivum, only contained celiac disease epitope DQ8-glia-α1/DQ8.5-glia-α1, which provided a theoretical basis for the low gluten toxicity wheat breeding. The transcriptome data of Th. elongatum exhibited differential expression in quantity and pattern in the same subfamily or different subfamilies. Dough rheological properties of T. aestivum-Th. elongatum disomic substitution (DS) line 1E(1D) showed higher peak height values than that of their parents, and DS6E(6D) exhibited fewer α-gliadins, which indicates the potential usage for wheat quality breeding. Conclusions Overall, this study provided a comprehensive overview of the prolamin gene family in Th. elongatum, and suggested a promising use of this species in the generation of improved wheat breeds intended for the human diet. |
| first_indexed | 2024-12-17T20:47:37Z |
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| id | doaj.art-7ddf8d64759845d3adc90109eb67e669 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-17T20:47:37Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-7ddf8d64759845d3adc90109eb67e6692022-12-21T21:33:08ZengBMCBMC Genomics1471-21642021-12-0122111210.1186/s12864-021-08088-xGenome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatumWenyang Ge0Yu Gao1Shoushen Xu2Xin Ma3Hongwei Wang4Lingrang Kong5Silong Sun6State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural UniversityState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural UniversityAbstract Background Prolamins, unique to Gramineae (grasses), play a key role in the human diet. Thinopyrum elongatum (syn. Agropyron elongatum or Lophopyrum elongatum), a grass of the Triticeae family with a diploid E genome (2n = 2x = 14), is genetically well-characterized, but little is known about its prolamin genes and the relationships with homologous loci in the Triticeae species. Results In this study, a total of 19 α-gliadin, 9 γ-gliadin, 19 ω-gliadin, 2 high-molecular-weight glutenin subunit (HMW-GS), and 5 low-molecular-weight glutenin subunit (LMW-GS) genes were identified in the Th. elongatum genome. Micro-synteny and phylogenetic analysis revealed dynamic changes of prolamin gene regions and genetic affinities among Th. elongatum, Triticum aestivum, T. urartu and Aegilops tauschii. The Th. elongatum genome, like the B subgenome of T. aestivum, only contained celiac disease epitope DQ8-glia-α1/DQ8.5-glia-α1, which provided a theoretical basis for the low gluten toxicity wheat breeding. The transcriptome data of Th. elongatum exhibited differential expression in quantity and pattern in the same subfamily or different subfamilies. Dough rheological properties of T. aestivum-Th. elongatum disomic substitution (DS) line 1E(1D) showed higher peak height values than that of their parents, and DS6E(6D) exhibited fewer α-gliadins, which indicates the potential usage for wheat quality breeding. Conclusions Overall, this study provided a comprehensive overview of the prolamin gene family in Th. elongatum, and suggested a promising use of this species in the generation of improved wheat breeds intended for the human diet.https://doi.org/10.1186/s12864-021-08088-xThinopyrum elongatumProlaminsExpressionEvolutionCeliac disease |
| spellingShingle | Wenyang Ge Yu Gao Shoushen Xu Xin Ma Hongwei Wang Lingrang Kong Silong Sun Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum BMC Genomics Thinopyrum elongatum Prolamins Expression Evolution Celiac disease |
| title | Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum |
| title_full | Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum |
| title_fullStr | Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum |
| title_full_unstemmed | Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum |
| title_short | Genome-wide identification, characteristics and expression of the prolamin genes in Thinopyrum elongatum |
| title_sort | genome wide identification characteristics and expression of the prolamin genes in thinopyrum elongatum |
| topic | Thinopyrum elongatum Prolamins Expression Evolution Celiac disease |
| url | https://doi.org/10.1186/s12864-021-08088-x |
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