Survival model database of human digestive system cells exposed to electroporation pulses: An in vitro and in silico study
Background and objectivesThis study aimed to establish a mathematical survival model database containing cell-specific coefficients from human digestive system cells exposed to electroporation pulses (EPs).Materials and methodsA total of 20 types of human digestive system cell lines were selected to...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Public Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2022.948562/full |
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| author | Xuan Han Xuan Han Nana Zhang Nana Zhang Yuchi Zhang Yuchi Zhang Zhuoqun Li Zhuoqun Li Yingxue Wang Yingxue Wang Lujing Mao Lujing Mao Tianshuai He Tianshuai He Qingshan Li Qingshan Li Jiawen Zhao Xue Chen Yixuan Li Zitong Qin Yi Lv Yi Lv Yi Lv Fenggang Ren Fenggang Ren |
| author_facet | Xuan Han Xuan Han Nana Zhang Nana Zhang Yuchi Zhang Yuchi Zhang Zhuoqun Li Zhuoqun Li Yingxue Wang Yingxue Wang Lujing Mao Lujing Mao Tianshuai He Tianshuai He Qingshan Li Qingshan Li Jiawen Zhao Xue Chen Yixuan Li Zitong Qin Yi Lv Yi Lv Yi Lv Fenggang Ren Fenggang Ren |
| author_sort | Xuan Han |
| collection | DOAJ |
| description | Background and objectivesThis study aimed to establish a mathematical survival model database containing cell-specific coefficients from human digestive system cells exposed to electroporation pulses (EPs).Materials and methodsA total of 20 types of human digestive system cell lines were selected to investigate the effect of EPs on cell viability. Cell viability was measured after exposure to various pulse settings, and a cell survival model was established using the Peleg–Fermi model. Next, the cell-specific coefficients of each cell line were determined.ResultsCell viability tended to decrease when exposed to stronger electric field strength (EFS), longer pulse duration, and more pulse number, but the decreasing tendency varied among different cell lines. When exposed to a lower EFS (<1,000 V/cm), only a slight decrease in cell viability occurred. All cell lines showed a similar tendency: the extent of electrical injury (EI) increased with the increase in pulse number and duration. However, there existed differences in heat sensitivity among organs.ConclusionsThis database can be used for the application of electroporation-based treatment (EBT) in the digestive system to predict cell survival and tissue injury distribution during the treatment. |
| first_indexed | 2024-04-11T12:25:51Z |
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| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-04-11T12:25:51Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj.art-7ddfe1decc0641ee98d1e36385812e1c2022-12-22T04:23:57ZengFrontiers Media S.A.Frontiers in Public Health2296-25652022-09-011010.3389/fpubh.2022.948562948562Survival model database of human digestive system cells exposed to electroporation pulses: An in vitro and in silico studyXuan Han0Xuan Han1Nana Zhang2Nana Zhang3Yuchi Zhang4Yuchi Zhang5Zhuoqun Li6Zhuoqun Li7Yingxue Wang8Yingxue Wang9Lujing Mao10Lujing Mao11Tianshuai He12Tianshuai He13Qingshan Li14Qingshan Li15Jiawen Zhao16Xue Chen17Yixuan Li18Zitong Qin19Yi Lv20Yi Lv21Yi Lv22Fenggang Ren23Fenggang Ren24Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaInstitute of Regenerative and Reconstructive Medicine, Med-X Institute, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaInstitute of Regenerative and Reconstructive Medicine, Med-X Institute, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaInstitute of Regenerative and Reconstructive Medicine, Med-X Institute, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaInstitute of Regenerative and Reconstructive Medicine, Med-X Institute, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaInstitute of Regenerative and Reconstructive Medicine, Med-X Institute, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, ChinaBackground and objectivesThis study aimed to establish a mathematical survival model database containing cell-specific coefficients from human digestive system cells exposed to electroporation pulses (EPs).Materials and methodsA total of 20 types of human digestive system cell lines were selected to investigate the effect of EPs on cell viability. Cell viability was measured after exposure to various pulse settings, and a cell survival model was established using the Peleg–Fermi model. Next, the cell-specific coefficients of each cell line were determined.ResultsCell viability tended to decrease when exposed to stronger electric field strength (EFS), longer pulse duration, and more pulse number, but the decreasing tendency varied among different cell lines. When exposed to a lower EFS (<1,000 V/cm), only a slight decrease in cell viability occurred. All cell lines showed a similar tendency: the extent of electrical injury (EI) increased with the increase in pulse number and duration. However, there existed differences in heat sensitivity among organs.ConclusionsThis database can be used for the application of electroporation-based treatment (EBT) in the digestive system to predict cell survival and tissue injury distribution during the treatment.https://www.frontiersin.org/articles/10.3389/fpubh.2022.948562/fullelectroporation pulsesirreversible electroporationdigestive systemnumerical analysismathematical model |
| spellingShingle | Xuan Han Xuan Han Nana Zhang Nana Zhang Yuchi Zhang Yuchi Zhang Zhuoqun Li Zhuoqun Li Yingxue Wang Yingxue Wang Lujing Mao Lujing Mao Tianshuai He Tianshuai He Qingshan Li Qingshan Li Jiawen Zhao Xue Chen Yixuan Li Zitong Qin Yi Lv Yi Lv Yi Lv Fenggang Ren Fenggang Ren Survival model database of human digestive system cells exposed to electroporation pulses: An in vitro and in silico study Frontiers in Public Health electroporation pulses irreversible electroporation digestive system numerical analysis mathematical model |
| title | Survival model database of human digestive system cells exposed to electroporation pulses: An in vitro and in silico study |
| title_full | Survival model database of human digestive system cells exposed to electroporation pulses: An in vitro and in silico study |
| title_fullStr | Survival model database of human digestive system cells exposed to electroporation pulses: An in vitro and in silico study |
| title_full_unstemmed | Survival model database of human digestive system cells exposed to electroporation pulses: An in vitro and in silico study |
| title_short | Survival model database of human digestive system cells exposed to electroporation pulses: An in vitro and in silico study |
| title_sort | survival model database of human digestive system cells exposed to electroporation pulses an in vitro and in silico study |
| topic | electroporation pulses irreversible electroporation digestive system numerical analysis mathematical model |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2022.948562/full |
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