Deletion of FaeG alleviated Enterotoxigenic Escherichia coli F4ac-induced apoptosis in the intestine
Abstract Enterotoxigenic Escherichia coli (ETEC) F4ac is a major constraint to the development of the pig industry, which is causing newborn and post-weaning piglets diarrhea. Previous studies proved that FaeG is the major fimbrial subunit of F4ac E. coli and efficient for bacterial adherence and re...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-03-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13568-021-01201-z |
| _version_ | 1818416253175332864 |
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| author | Pengpeng Xia Yunping Wu Siqi Lian Guomei Quan Yiting Wang Guoqiang Zhu |
| author_facet | Pengpeng Xia Yunping Wu Siqi Lian Guomei Quan Yiting Wang Guoqiang Zhu |
| author_sort | Pengpeng Xia |
| collection | DOAJ |
| description | Abstract Enterotoxigenic Escherichia coli (ETEC) F4ac is a major constraint to the development of the pig industry, which is causing newborn and post-weaning piglets diarrhea. Previous studies proved that FaeG is the major fimbrial subunit of F4ac E. coli and efficient for bacterial adherence and receptor recognition. Here we show that the faeG deletion attenuates both the clinical symptoms of F4ac infection and the F4ac-induced intestinal mucosal damage in piglets. Antibody microarray analysis and the detection of mRNA expression using porcine neonatal jejunal IPEC-J2 cells also determined that the absence of FaeG subunit alleviated the F4ac promoted apoptosis in the intestinal epithelial cells. Thus, targeted depletion of FaeG is still beneficial for the prevention or treatment of F4ac infection. |
| first_indexed | 2024-12-14T11:47:57Z |
| format | Article |
| id | doaj.art-7de1f925ea744a8e8d21487d49d299fd |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-14T11:47:57Z |
| publishDate | 2021-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-7de1f925ea744a8e8d21487d49d299fd2022-12-21T23:02:29ZengSpringerOpenAMB Express2191-08552021-03-011111810.1186/s13568-021-01201-zDeletion of FaeG alleviated Enterotoxigenic Escherichia coli F4ac-induced apoptosis in the intestinePengpeng Xia0Yunping Wu1Siqi Lian2Guomei Quan3Yiting Wang4Guoqiang Zhu5College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou UniversityCollege of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou UniversityCollege of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou UniversityCollege of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou UniversityCollege of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou UniversityCollege of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou UniversityAbstract Enterotoxigenic Escherichia coli (ETEC) F4ac is a major constraint to the development of the pig industry, which is causing newborn and post-weaning piglets diarrhea. Previous studies proved that FaeG is the major fimbrial subunit of F4ac E. coli and efficient for bacterial adherence and receptor recognition. Here we show that the faeG deletion attenuates both the clinical symptoms of F4ac infection and the F4ac-induced intestinal mucosal damage in piglets. Antibody microarray analysis and the detection of mRNA expression using porcine neonatal jejunal IPEC-J2 cells also determined that the absence of FaeG subunit alleviated the F4ac promoted apoptosis in the intestinal epithelial cells. Thus, targeted depletion of FaeG is still beneficial for the prevention or treatment of F4ac infection.https://doi.org/10.1186/s13568-021-01201-zEnterotoxigenic Escherichia coli (ETEC) F4acFaeG subunitApoptosis |
| spellingShingle | Pengpeng Xia Yunping Wu Siqi Lian Guomei Quan Yiting Wang Guoqiang Zhu Deletion of FaeG alleviated Enterotoxigenic Escherichia coli F4ac-induced apoptosis in the intestine AMB Express Enterotoxigenic Escherichia coli (ETEC) F4ac FaeG subunit Apoptosis |
| title | Deletion of FaeG alleviated Enterotoxigenic Escherichia coli F4ac-induced apoptosis in the intestine |
| title_full | Deletion of FaeG alleviated Enterotoxigenic Escherichia coli F4ac-induced apoptosis in the intestine |
| title_fullStr | Deletion of FaeG alleviated Enterotoxigenic Escherichia coli F4ac-induced apoptosis in the intestine |
| title_full_unstemmed | Deletion of FaeG alleviated Enterotoxigenic Escherichia coli F4ac-induced apoptosis in the intestine |
| title_short | Deletion of FaeG alleviated Enterotoxigenic Escherichia coli F4ac-induced apoptosis in the intestine |
| title_sort | deletion of faeg alleviated enterotoxigenic escherichia coli f4ac induced apoptosis in the intestine |
| topic | Enterotoxigenic Escherichia coli (ETEC) F4ac FaeG subunit Apoptosis |
| url | https://doi.org/10.1186/s13568-021-01201-z |
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