Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design
Abstract Docosahexaenoic acid (DHA) can improve human and animal health, particularly including anti-inflammatory, antioxidant, anticancer, neurological, and visual functions. Schizochytrium sp. is a marine heterotrophic protist producing oil with high DHA content, which is widely used in animal and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Biotechnology |
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| Online Access: | https://doi.org/10.1186/s12896-022-00769-z |
| _version_ | 1811203551052955648 |
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| author | Jun Ding Zilin Fu Yingkun Zhu Junhao He Lu Ma Dengpan Bu |
| author_facet | Jun Ding Zilin Fu Yingkun Zhu Junhao He Lu Ma Dengpan Bu |
| author_sort | Jun Ding |
| collection | DOAJ |
| description | Abstract Docosahexaenoic acid (DHA) can improve human and animal health, particularly including anti-inflammatory, antioxidant, anticancer, neurological, and visual functions. Schizochytrium sp. is a marine heterotrophic protist producing oil with high DHA content, which is widely used in animal and food production. However, different fermentation conditions have intensive impacts on the growth and DHA content of Schizochytrium sp. Thus, this study aimed to enhance the DHA yield and concentration of Schizochytrium sp. I-F-9 by optimizing the fermentation medium. First, a single-factor design was conducted to select a target carbon and nitrogen source from several generic sources (glucose, sucrose, glycerol, maltose, corn syrup, yeast extract, urea, peptone, and ammonium sulfate). The Plackett–Burman design and the central composite design (CCD) were utilized to optimize the fermentation mediums. Schizochytrium sp. in 50-mL fermentation broth was cultured in a 250 mL shake flask at 28 °C and 200 rpm for 120 h before collecting the cell pellet. Subsequently, the cell walls were destroyed with hydrochloric acid to extract the fatty acid using n-hexane. The DHA content was detected by gas chromatography. The single-factor test indicated that glucose and peptone, respectively, significantly improved the DHA content of Schizochytrium sp. compared to the other carbon and nitrogen sources. Glucose, sodium glutamate, and sea crystal were the key factors affecting DHA production in the Plackett–Burman test (P = 0.0247). The CCD result showed that DHA production was elevated by 34.73% compared with the initial yield (from 6.18 ± 0.063 to 8.33 ± 0.052 g/L). Therefore, the results of this study demonstrated an efficient strategy to increase the yield and content of DHA of Schizochytrium sp. |
| first_indexed | 2024-04-12T02:57:16Z |
| format | Article |
| id | doaj.art-7de2c22596874cccb02b4ad766b06058 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-04-12T02:57:16Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-7de2c22596874cccb02b4ad766b060582022-12-22T03:50:46ZengBMCBMC Biotechnology1472-67502022-12-0122111210.1186/s12896-022-00769-zEnhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite designJun Ding0Zilin Fu1Yingkun Zhu2Junhao He3Lu Ma4Dengpan Bu5State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesAbstract Docosahexaenoic acid (DHA) can improve human and animal health, particularly including anti-inflammatory, antioxidant, anticancer, neurological, and visual functions. Schizochytrium sp. is a marine heterotrophic protist producing oil with high DHA content, which is widely used in animal and food production. However, different fermentation conditions have intensive impacts on the growth and DHA content of Schizochytrium sp. Thus, this study aimed to enhance the DHA yield and concentration of Schizochytrium sp. I-F-9 by optimizing the fermentation medium. First, a single-factor design was conducted to select a target carbon and nitrogen source from several generic sources (glucose, sucrose, glycerol, maltose, corn syrup, yeast extract, urea, peptone, and ammonium sulfate). The Plackett–Burman design and the central composite design (CCD) were utilized to optimize the fermentation mediums. Schizochytrium sp. in 50-mL fermentation broth was cultured in a 250 mL shake flask at 28 °C and 200 rpm for 120 h before collecting the cell pellet. Subsequently, the cell walls were destroyed with hydrochloric acid to extract the fatty acid using n-hexane. The DHA content was detected by gas chromatography. The single-factor test indicated that glucose and peptone, respectively, significantly improved the DHA content of Schizochytrium sp. compared to the other carbon and nitrogen sources. Glucose, sodium glutamate, and sea crystal were the key factors affecting DHA production in the Plackett–Burman test (P = 0.0247). The CCD result showed that DHA production was elevated by 34.73% compared with the initial yield (from 6.18 ± 0.063 to 8.33 ± 0.052 g/L). Therefore, the results of this study demonstrated an efficient strategy to increase the yield and content of DHA of Schizochytrium sp.https://doi.org/10.1186/s12896-022-00769-zCenter composite designDHAPlackett–Burman designSchizochytrium sp. |
| spellingShingle | Jun Ding Zilin Fu Yingkun Zhu Junhao He Lu Ma Dengpan Bu Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design BMC Biotechnology Center composite design DHA Plackett–Burman design Schizochytrium sp. |
| title | Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design |
| title_full | Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design |
| title_fullStr | Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design |
| title_full_unstemmed | Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design |
| title_short | Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design |
| title_sort | enhancing docosahexaenoic acid production of schizochytrium sp by optimizing fermentation using central composite design |
| topic | Center composite design DHA Plackett–Burman design Schizochytrium sp. |
| url | https://doi.org/10.1186/s12896-022-00769-z |
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