A Potential Biocontrol Agent Streptomycesviolaceusniger AC12AB for Managing Potato Common Scab
Potato common scab (PCS) is an economically important disease worldwide. In this study we demonstrated the possible role of Streptomyces violaceusniger AC12AB in controlling PCS. Isolates of Streptomyces scabies were obtained from CS infected tubers collected from Maine United States, which were con...
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00202/full |
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| author | Arslan Sarwar Zakia Latif Songya Zhang Jianjun Hao Andreas Bechthold |
| author_facet | Arslan Sarwar Zakia Latif Songya Zhang Jianjun Hao Andreas Bechthold |
| author_sort | Arslan Sarwar |
| collection | DOAJ |
| description | Potato common scab (PCS) is an economically important disease worldwide. In this study we demonstrated the possible role of Streptomyces violaceusniger AC12AB in controlling PCS. Isolates of Streptomyces scabies were obtained from CS infected tubers collected from Maine United States, which were confirmed by morphological and molecular analysis including 16S rRNA sequencing and RFLP analysis of amplified 16S-23S ITS. Pathogenicity assays related genes including txtAB, nec1, and tomA were also identified in all S. scabies strains through PCR reaction. An antagonistic bacterial strain was isolated from soil in Punjab and identified as S. violaceusniger AC12AB based on 16S rRNA sequencing analysis. Methanolic extract of S. violaceusniger AC12AB contained azalomycin RS-22A which was confirmed by 1H and 13C-NMR, 1H/1H-COSY, HMBC and HMQC techniques. S. violaceusniger AC12AB exhibited plant growth promotion attributes including Indole-3-acetic acid production with 17 μgmL-1 titers, siderophores production, nitrogen fixation and phosphates solubilization potential. When tubers were inoculated with S. violaceusniger AC12AB, significant (P < 0.05) PCS disease reduction up to 90% was observed in greenhouse and field trials, respectively. Likewise, S. violaceusniger AC12AB significantly (P < 0.05) increased potato crop up to 26.8% in field trial. Therefore, plant growth promoting S. violaceusniger AC12AB could provide a dual benefit by decreasing PCS disease severity and increasing potato yield as an effective and inexpensive alternative strategy to manage this disease. |
| first_indexed | 2024-12-17T07:31:51Z |
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| language | English |
| last_indexed | 2024-12-17T07:31:51Z |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-7de42a32ff814040a19f002afa091a232022-12-21T21:58:28ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-02-011010.3389/fmicb.2019.00202438567A Potential Biocontrol Agent Streptomycesviolaceusniger AC12AB for Managing Potato Common ScabArslan Sarwar0Zakia Latif1Songya Zhang2Jianjun Hao3Andreas Bechthold4Department of Microbiology, Faculty of Life Sciences, University of Central Punjab, Lahore, PakistanDepartment of Microbiology and Molecular Genetics, University of the Punjab, Lahore, PakistanDepartment of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, University of Freiburg, Freiburg im Breisgau, GermanySchool of Food and Agriculture, The University of Maine, Orono, ME, United StatesDepartment of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, University of Freiburg, Freiburg im Breisgau, GermanyPotato common scab (PCS) is an economically important disease worldwide. In this study we demonstrated the possible role of Streptomyces violaceusniger AC12AB in controlling PCS. Isolates of Streptomyces scabies were obtained from CS infected tubers collected from Maine United States, which were confirmed by morphological and molecular analysis including 16S rRNA sequencing and RFLP analysis of amplified 16S-23S ITS. Pathogenicity assays related genes including txtAB, nec1, and tomA were also identified in all S. scabies strains through PCR reaction. An antagonistic bacterial strain was isolated from soil in Punjab and identified as S. violaceusniger AC12AB based on 16S rRNA sequencing analysis. Methanolic extract of S. violaceusniger AC12AB contained azalomycin RS-22A which was confirmed by 1H and 13C-NMR, 1H/1H-COSY, HMBC and HMQC techniques. S. violaceusniger AC12AB exhibited plant growth promotion attributes including Indole-3-acetic acid production with 17 μgmL-1 titers, siderophores production, nitrogen fixation and phosphates solubilization potential. When tubers were inoculated with S. violaceusniger AC12AB, significant (P < 0.05) PCS disease reduction up to 90% was observed in greenhouse and field trials, respectively. Likewise, S. violaceusniger AC12AB significantly (P < 0.05) increased potato crop up to 26.8% in field trial. Therefore, plant growth promoting S. violaceusniger AC12AB could provide a dual benefit by decreasing PCS disease severity and increasing potato yield as an effective and inexpensive alternative strategy to manage this disease.https://www.frontiersin.org/article/10.3389/fmicb.2019.00202/fullStreptomyces scabiesbiological controlplant growth promoting Streptomycesantagonistic Streptomycespotato common scab |
| spellingShingle | Arslan Sarwar Zakia Latif Songya Zhang Jianjun Hao Andreas Bechthold A Potential Biocontrol Agent Streptomycesviolaceusniger AC12AB for Managing Potato Common Scab Frontiers in Microbiology Streptomyces scabies biological control plant growth promoting Streptomyces antagonistic Streptomyces potato common scab |
| title | A Potential Biocontrol Agent Streptomycesviolaceusniger AC12AB for Managing Potato Common Scab |
| title_full | A Potential Biocontrol Agent Streptomycesviolaceusniger AC12AB for Managing Potato Common Scab |
| title_fullStr | A Potential Biocontrol Agent Streptomycesviolaceusniger AC12AB for Managing Potato Common Scab |
| title_full_unstemmed | A Potential Biocontrol Agent Streptomycesviolaceusniger AC12AB for Managing Potato Common Scab |
| title_short | A Potential Biocontrol Agent Streptomycesviolaceusniger AC12AB for Managing Potato Common Scab |
| title_sort | potential biocontrol agent streptomycesviolaceusniger ac12ab for managing potato common scab |
| topic | Streptomyces scabies biological control plant growth promoting Streptomyces antagonistic Streptomyces potato common scab |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00202/full |
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