The effect of purple passion fruit juice on superoxide dismutase and malondialdehyde levels in hypercholesterolemic rats
Background Hypercholesterolemia due to a high cholesterol diet can increase free radicals resulting in oxidative stress. Superoxide dismutase (SOD) and malondialdehyde (MDA) have been used as the study markers of oxidative stress in cases of hypercholesterolemia. Purple passion fruit contains vario...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine Trisakti University
2022-06-01
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| Series: | Universa Medicina |
| Subjects: | |
| Online Access: | https://univmed.org/ejurnal/index.php/medicina/article/view/1280 |
| _version_ | 1828153731734044672 |
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| author | Alfi Muntafiah Johanes Hasian Siahaan Sofyan Hardi Dody Novrial Hernayanti Hernayanti |
| author_facet | Alfi Muntafiah Johanes Hasian Siahaan Sofyan Hardi Dody Novrial Hernayanti Hernayanti |
| author_sort | Alfi Muntafiah |
| collection | DOAJ |
| description |
Background
Hypercholesterolemia due to a high cholesterol diet can increase free radicals resulting in oxidative stress. Superoxide dismutase (SOD) and malondialdehyde (MDA) have been used as the study markers of oxidative stress in cases of hypercholesterolemia. Purple passion fruit contains various compounds that may reduce free radicals. This study aimed to determine the effect of purple passion fruit juice on SOD and MDA levels in hypercholesterolemic rats.
Methods
An experimental analysis with post-test only control group design involving 28 male Wistar rats. They were divided into 4 groups: normal control (K1), hypercholesterolemic control (K2), purple passion fruit juice treatment at 4.2 mL/200 gBW/day (K3), and simvastatin treatment at 0.018 mg/200 gBW/day (K4). The purple passion fruit juice at 4.2 mL/200 gBW/day was administered for 14 days. SOD levels were examined by enzymatic colorimetric methods using the Ransod kit and MDA levels by the TBARS method.
Results
The Kruskal-Wallis test showed a significant difference in SOD levels between the tested groups (p<0.05). One-way ANOVA test for MDA levels showed a significant difference (p<0.05). Post Hoc test (Mann-Whitney for SOD and LSD for MDA levels) also showed significant differences: K1 vs. K2, K2 vs. K3, K2 vs. K4, and K3 vs. K4 (p<0.05).
Conclusion
This study demonstrated that purple passion fruit juice significantly increases the SOD and lowers the MDA level in hypercholesterolemic male Wistar rats. Consumption of purple passion fruit juice may help to modulate oxidative stress caused by hypercholesterolemia in rats.
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| first_indexed | 2024-04-11T22:29:22Z |
| format | Article |
| id | doaj.art-7de854bce3584cf785b7d55b0dafb6a8 |
| institution | Directory Open Access Journal |
| issn | 1907-3062 2407-2230 |
| language | English |
| last_indexed | 2024-04-11T22:29:22Z |
| publishDate | 2022-06-01 |
| publisher | Faculty of Medicine Trisakti University |
| record_format | Article |
| series | Universa Medicina |
| spelling | doaj.art-7de854bce3584cf785b7d55b0dafb6a82022-12-22T03:59:31ZengFaculty of Medicine Trisakti UniversityUniversa Medicina1907-30622407-22302022-06-0141210.18051/UnivMed.2022.v41.139-148The effect of purple passion fruit juice on superoxide dismutase and malondialdehyde levels in hypercholesterolemic ratsAlfi Muntafiah0Johanes Hasian Siahaan1Sofyan Hardi2Dody Novrial3Hernayanti Hernayanti4Department of Biochemistry, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, IndonesiaDepartment of Biochemistry, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, IndonesiaDepartment of Biochemistry, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, IndonesiaDepartment of Anatomical Pathology, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, IndonesiaDepartment of Biology, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Indonesia Background Hypercholesterolemia due to a high cholesterol diet can increase free radicals resulting in oxidative stress. Superoxide dismutase (SOD) and malondialdehyde (MDA) have been used as the study markers of oxidative stress in cases of hypercholesterolemia. Purple passion fruit contains various compounds that may reduce free radicals. This study aimed to determine the effect of purple passion fruit juice on SOD and MDA levels in hypercholesterolemic rats. Methods An experimental analysis with post-test only control group design involving 28 male Wistar rats. They were divided into 4 groups: normal control (K1), hypercholesterolemic control (K2), purple passion fruit juice treatment at 4.2 mL/200 gBW/day (K3), and simvastatin treatment at 0.018 mg/200 gBW/day (K4). The purple passion fruit juice at 4.2 mL/200 gBW/day was administered for 14 days. SOD levels were examined by enzymatic colorimetric methods using the Ransod kit and MDA levels by the TBARS method. Results The Kruskal-Wallis test showed a significant difference in SOD levels between the tested groups (p<0.05). One-way ANOVA test for MDA levels showed a significant difference (p<0.05). Post Hoc test (Mann-Whitney for SOD and LSD for MDA levels) also showed significant differences: K1 vs. K2, K2 vs. K3, K2 vs. K4, and K3 vs. K4 (p<0.05). Conclusion This study demonstrated that purple passion fruit juice significantly increases the SOD and lowers the MDA level in hypercholesterolemic male Wistar rats. Consumption of purple passion fruit juice may help to modulate oxidative stress caused by hypercholesterolemia in rats. https://univmed.org/ejurnal/index.php/medicina/article/view/1280Hypercholesterolemiamalondialdehydeoxidative stressPassiflora edulis var edulissuperoxide dismutaserats |
| spellingShingle | Alfi Muntafiah Johanes Hasian Siahaan Sofyan Hardi Dody Novrial Hernayanti Hernayanti The effect of purple passion fruit juice on superoxide dismutase and malondialdehyde levels in hypercholesterolemic rats Universa Medicina Hypercholesterolemia malondialdehyde oxidative stress Passiflora edulis var edulis superoxide dismutase rats |
| title | The effect of purple passion fruit juice on superoxide dismutase and malondialdehyde levels in hypercholesterolemic rats |
| title_full | The effect of purple passion fruit juice on superoxide dismutase and malondialdehyde levels in hypercholesterolemic rats |
| title_fullStr | The effect of purple passion fruit juice on superoxide dismutase and malondialdehyde levels in hypercholesterolemic rats |
| title_full_unstemmed | The effect of purple passion fruit juice on superoxide dismutase and malondialdehyde levels in hypercholesterolemic rats |
| title_short | The effect of purple passion fruit juice on superoxide dismutase and malondialdehyde levels in hypercholesterolemic rats |
| title_sort | effect of purple passion fruit juice on superoxide dismutase and malondialdehyde levels in hypercholesterolemic rats |
| topic | Hypercholesterolemia malondialdehyde oxidative stress Passiflora edulis var edulis superoxide dismutase rats |
| url | https://univmed.org/ejurnal/index.php/medicina/article/view/1280 |
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