Experimental diets dictate the metabolic benefits of probiotics in obesity
ABSTRACTGrowing evidence supports the use of probiotics to prevent or mitigate obesity-related dysmetabolism and non-alcoholic fatty liver disease (NAFLD). However, frequent reports of responders versus non-responders to probiotic treatment warrant a better understanding of key modifiers of host–mic...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Gut Microbes |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2023.2192547 |
| _version_ | 1797236659075416064 |
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| author | Ida Søgaard Larsen Béatrice S.-Y. Choi Bandik Föh Nanna Ny Kristensen Adia Ouellette Rune Falkenberg Haller Peter Bjarke Olsen Delphine Saulnier Christian Sina Benjamin A. H. Jensen André Marette |
| author_facet | Ida Søgaard Larsen Béatrice S.-Y. Choi Bandik Föh Nanna Ny Kristensen Adia Ouellette Rune Falkenberg Haller Peter Bjarke Olsen Delphine Saulnier Christian Sina Benjamin A. H. Jensen André Marette |
| author_sort | Ida Søgaard Larsen |
| collection | DOAJ |
| description | ABSTRACTGrowing evidence supports the use of probiotics to prevent or mitigate obesity-related dysmetabolism and non-alcoholic fatty liver disease (NAFLD). However, frequent reports of responders versus non-responders to probiotic treatment warrant a better understanding of key modifiers of host–microbe interactions. The influence of host diet on probiotic efficacy, in particular against metabolic diseases, remains elusive. We fed C57BL6/J mice a low fat reference diet or one of two energy-matched high fat and high sucrose diets for 12 weeks; a classical high fat diet (HFD) and a customized fast food-mimicking diet (FFMD). During the studies, mice fed either obesogenic diet were gavaged daily with one of two probiotic lactic acid bacteria (LAB) strains previously classified as Lactobaccillus, namely Limosilactobacillus reuteri (L. reuteri)or Lacticaseibacillus paracaseisubsp. paracasei (L. paracasei), or vehicle. The tested probiotics exhibited a reproducible efficacy but dichotomous response according to the obesogenic diets used. Indeed, L. paracaseiprevented weight gain, improved insulin sensitivity, and protected against NAFLD development in mice fed HFD, but not FFMD. Conversely, L. reuteri improved glucoregulatory capacity, reduced NAFLD development, and increased distal gut bile acid levels associated with changes in predicted functions of the gut microbiota exclusively in the context of FFMD-feeding. We found that the probiotic efficacy of two LAB strains is highly dependent on experimental obesogenic diets. These findings highlight the need to carefully consider the confounding impact of diet in order to improve both the reproducibility of preclinical probiotic studies and their clinical research translatability. |
| first_indexed | 2024-03-11T14:20:26Z |
| format | Article |
| id | doaj.art-7de96919f8fc4a578b4f7c0db3883cee |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-04-24T17:07:22Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-7de96919f8fc4a578b4f7c0db3883cee2024-03-28T22:38:20ZengTaylor & Francis GroupGut Microbes1949-09761949-09842023-12-0115110.1080/19490976.2023.2192547Experimental diets dictate the metabolic benefits of probiotics in obesityIda Søgaard Larsen0Béatrice S.-Y. Choi1Bandik Föh2Nanna Ny Kristensen3Adia Ouellette4Rune Falkenberg Haller5Peter Bjarke Olsen6Delphine Saulnier7Christian Sina8Benjamin A. H. Jensen9André Marette10Quebec Heart and Lung Institute, Faculty of Medicine, and Institute of Nutrition and Functional Foods (INAF), Laval University, Quebec, QC, CanadaQuebec Heart and Lung Institute, Faculty of Medicine, and Institute of Nutrition and Functional Foods (INAF), Laval University, Quebec, QC, CanadaInstitute of Nutritional Medicine, University of Lübeck, Lübeck, GermanyNovozymes A/S, Bagsvaerd, DenmarkQuebec Heart and Lung Institute, Faculty of Medicine, and Institute of Nutrition and Functional Foods (INAF), Laval University, Quebec, QC, CanadaNovozymes A/S, Bagsvaerd, DenmarkNovozymes A/S, Bagsvaerd, DenmarkNovozymes Berlin GmbH, Berlin, GermanyInstitute of Nutritional Medicine, University of Lübeck, Lübeck, GermanyQuebec Heart and Lung Institute, Faculty of Medicine, and Institute of Nutrition and Functional Foods (INAF), Laval University, Quebec, QC, CanadaQuebec Heart and Lung Institute, Faculty of Medicine, and Institute of Nutrition and Functional Foods (INAF), Laval University, Quebec, QC, CanadaABSTRACTGrowing evidence supports the use of probiotics to prevent or mitigate obesity-related dysmetabolism and non-alcoholic fatty liver disease (NAFLD). However, frequent reports of responders versus non-responders to probiotic treatment warrant a better understanding of key modifiers of host–microbe interactions. The influence of host diet on probiotic efficacy, in particular against metabolic diseases, remains elusive. We fed C57BL6/J mice a low fat reference diet or one of two energy-matched high fat and high sucrose diets for 12 weeks; a classical high fat diet (HFD) and a customized fast food-mimicking diet (FFMD). During the studies, mice fed either obesogenic diet were gavaged daily with one of two probiotic lactic acid bacteria (LAB) strains previously classified as Lactobaccillus, namely Limosilactobacillus reuteri (L. reuteri)or Lacticaseibacillus paracaseisubsp. paracasei (L. paracasei), or vehicle. The tested probiotics exhibited a reproducible efficacy but dichotomous response according to the obesogenic diets used. Indeed, L. paracaseiprevented weight gain, improved insulin sensitivity, and protected against NAFLD development in mice fed HFD, but not FFMD. Conversely, L. reuteri improved glucoregulatory capacity, reduced NAFLD development, and increased distal gut bile acid levels associated with changes in predicted functions of the gut microbiota exclusively in the context of FFMD-feeding. We found that the probiotic efficacy of two LAB strains is highly dependent on experimental obesogenic diets. These findings highlight the need to carefully consider the confounding impact of diet in order to improve both the reproducibility of preclinical probiotic studies and their clinical research translatability.https://www.tandfonline.com/doi/10.1080/19490976.2023.2192547Probioticsdiet-induced obesityinsulin resistancemicrobiotadietary interactionNAFLD |
| spellingShingle | Ida Søgaard Larsen Béatrice S.-Y. Choi Bandik Föh Nanna Ny Kristensen Adia Ouellette Rune Falkenberg Haller Peter Bjarke Olsen Delphine Saulnier Christian Sina Benjamin A. H. Jensen André Marette Experimental diets dictate the metabolic benefits of probiotics in obesity Gut Microbes Probiotics diet-induced obesity insulin resistance microbiota dietary interaction NAFLD |
| title | Experimental diets dictate the metabolic benefits of probiotics in obesity |
| title_full | Experimental diets dictate the metabolic benefits of probiotics in obesity |
| title_fullStr | Experimental diets dictate the metabolic benefits of probiotics in obesity |
| title_full_unstemmed | Experimental diets dictate the metabolic benefits of probiotics in obesity |
| title_short | Experimental diets dictate the metabolic benefits of probiotics in obesity |
| title_sort | experimental diets dictate the metabolic benefits of probiotics in obesity |
| topic | Probiotics diet-induced obesity insulin resistance microbiota dietary interaction NAFLD |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2023.2192547 |
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