Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose.
The efficient use of hemicellulose in the plant cell wall is critical for the economic conversion of plant biomass to renewable fuels and chemicals. Previously, the yeast Saccharomyces cerevisiae has been engineered to convert the hemicellulose-derived pentose sugars xylose and arabinose to d-xylulo...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4918971?pdf=render |
| _version_ | 1819100115942506496 |
|---|---|
| author | Kulika Chomvong Stefan Bauer Daniel I Benjamin Xin Li Daniel K Nomura Jamie H D Cate |
| author_facet | Kulika Chomvong Stefan Bauer Daniel I Benjamin Xin Li Daniel K Nomura Jamie H D Cate |
| author_sort | Kulika Chomvong |
| collection | DOAJ |
| description | The efficient use of hemicellulose in the plant cell wall is critical for the economic conversion of plant biomass to renewable fuels and chemicals. Previously, the yeast Saccharomyces cerevisiae has been engineered to convert the hemicellulose-derived pentose sugars xylose and arabinose to d-xylulose-5-phosphate for conversion via the pentose phosphate pathway (PPP). However, efficient pentose utilization requires PPP optimization and may interfere with its roles in NADPH and pentose production. Here, we developed an alternative xylose utilization pathway that largely bypasses the PPP. In the new pathway, d-xylulose is converted to d-xylulose-1-phosphate, a novel metabolite to S. cerevisiae, which is then cleaved to glycolaldehyde and dihydroxyacetone phosphate. This synthetic pathway served as a platform for the biosynthesis of ethanol and ethylene glycol. The use of d-xylulose-1-phosphate as an entry point for xylose metabolism opens the way for optimizing chemical conversion of pentose sugars in S. cerevisiae in a modular fashion. |
| first_indexed | 2024-12-22T00:57:39Z |
| format | Article |
| id | doaj.art-7de9e49c68ed4601a6141a869fee2d14 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T00:57:39Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7de9e49c68ed4601a6141a869fee2d142022-12-21T18:44:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01116e015811110.1371/journal.pone.0158111Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose.Kulika ChomvongStefan BauerDaniel I BenjaminXin LiDaniel K NomuraJamie H D CateThe efficient use of hemicellulose in the plant cell wall is critical for the economic conversion of plant biomass to renewable fuels and chemicals. Previously, the yeast Saccharomyces cerevisiae has been engineered to convert the hemicellulose-derived pentose sugars xylose and arabinose to d-xylulose-5-phosphate for conversion via the pentose phosphate pathway (PPP). However, efficient pentose utilization requires PPP optimization and may interfere with its roles in NADPH and pentose production. Here, we developed an alternative xylose utilization pathway that largely bypasses the PPP. In the new pathway, d-xylulose is converted to d-xylulose-1-phosphate, a novel metabolite to S. cerevisiae, which is then cleaved to glycolaldehyde and dihydroxyacetone phosphate. This synthetic pathway served as a platform for the biosynthesis of ethanol and ethylene glycol. The use of d-xylulose-1-phosphate as an entry point for xylose metabolism opens the way for optimizing chemical conversion of pentose sugars in S. cerevisiae in a modular fashion.http://europepmc.org/articles/PMC4918971?pdf=render |
| spellingShingle | Kulika Chomvong Stefan Bauer Daniel I Benjamin Xin Li Daniel K Nomura Jamie H D Cate Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose. PLoS ONE |
| title | Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose. |
| title_full | Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose. |
| title_fullStr | Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose. |
| title_full_unstemmed | Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose. |
| title_short | Bypassing the Pentose Phosphate Pathway: Towards Modular Utilization of Xylose. |
| title_sort | bypassing the pentose phosphate pathway towards modular utilization of xylose |
| url | http://europepmc.org/articles/PMC4918971?pdf=render |
| work_keys_str_mv | AT kulikachomvong bypassingthepentosephosphatepathwaytowardsmodularutilizationofxylose AT stefanbauer bypassingthepentosephosphatepathwaytowardsmodularutilizationofxylose AT danielibenjamin bypassingthepentosephosphatepathwaytowardsmodularutilizationofxylose AT xinli bypassingthepentosephosphatepathwaytowardsmodularutilizationofxylose AT danielknomura bypassingthepentosephosphatepathwaytowardsmodularutilizationofxylose AT jamiehdcate bypassingthepentosephosphatepathwaytowardsmodularutilizationofxylose |