Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis
Porous microspheres have the potential for use as injectable bone fillers to obviate the need for open surgery. Successful bone fillers must be able to support vascularisation since tissue engineering scaffolds often cease functioning soon after implantation due to a failure to vascularise rapidly....
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AIP Publishing LLC
2018-06-01
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| Series: | APL Bioengineering |
| Online Access: | http://dx.doi.org/10.1063/1.5008556 |
| _version_ | 1819111510683680768 |
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| author | Thomas E. Paterson Giulia Gigliobianco Colin Sherborne Nicola H. Green James M. Dugan Sheila MacNeil Gwendolen C. Reilly Frederik Claeyssens |
| author_facet | Thomas E. Paterson Giulia Gigliobianco Colin Sherborne Nicola H. Green James M. Dugan Sheila MacNeil Gwendolen C. Reilly Frederik Claeyssens |
| author_sort | Thomas E. Paterson |
| collection | DOAJ |
| description | Porous microspheres have the potential for use as injectable bone fillers to obviate the need for open surgery. Successful bone fillers must be able to support vascularisation since tissue engineering scaffolds often cease functioning soon after implantation due to a failure to vascularise rapidly. Here, we test the angiogenic potential of a tissue engineered bone filler based on a photocurable acrylate-based high internal phase emulsion (HIPE). Highly porous microspheres were fabricated via two processes, which were compared. One was taken forward and investigated for its ability to support human mesenchymal progenitor cells and angiogenesis in a chorioallantoic membrane (CAM) assay. Porous microspheres with either a narrow or broad size distribution were prepared via a T-junction microfluidic device or by a controlled stirred-tank reactor of the HIPE water in oil in water (w/o/w), respectively. Culture of human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells showed proliferation over 11 days and formation of cell-microsphere aggregates. In-vitro, hES-MP cells were found to migrate into microspheres through their surface pores over time. The presence of osteoblasts, differentiated from the hES-MP cells, was evidenced through the presence of collagen and calcium after 30 days. Microspheres pre-cultured with cells were implanted into CAM for 7 days and compared with control microspheres without pre-cultured cells. The hES-MP seeded microspheres supported greater angiogenesis, as measured by the number of blood vessels and bifurcations, while the empty scaffolds attracted host chick cell ingrowth. This investigation shows that controlled fabrication of porous microspheres has the potential to create an angiogenic, bone filling material for use as a cell delivery vehicle. |
| first_indexed | 2024-12-22T03:58:46Z |
| format | Article |
| id | doaj.art-7dec20bd7d6041fbbe41b781bd79a3d7 |
| institution | Directory Open Access Journal |
| issn | 2473-2877 |
| language | English |
| last_indexed | 2024-12-22T03:58:46Z |
| publishDate | 2018-06-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | APL Bioengineering |
| spelling | doaj.art-7dec20bd7d6041fbbe41b781bd79a3d72022-12-21T18:39:48ZengAIP Publishing LLCAPL Bioengineering2473-28772018-06-0122026103026103-1910.1063/1.5008556005802APBPorous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesisThomas E. Paterson0Giulia Gigliobianco1Colin Sherborne2Nicola H. Green3James M. Dugan4Sheila MacNeil5Gwendolen C. Reilly6Frederik Claeyssens7 Department of Materials Science and Engineering, Kroto Research Institute, Broad Lane, University of Sheffield, Sheffield S3 7HQ, United Kingdom Department of Materials Science and Engineering, Kroto Research Institute, Broad Lane, University of Sheffield, Sheffield S3 7HQ, United Kingdom Department of Materials Science and Engineering, Kroto Research Institute, Broad Lane, University of Sheffield, Sheffield S3 7HQ, United Kingdom Department of Materials Science and Engineering, Kroto Research Institute, Broad Lane, University of Sheffield, Sheffield S3 7HQ, United Kingdom Department of Materials Science and Engineering, Kroto Research Institute, Broad Lane, University of Sheffield, Sheffield S3 7HQ, United Kingdom Department of Materials Science and Engineering, Kroto Research Institute, Broad Lane, University of Sheffield, Sheffield S3 7HQ, United Kingdom Department of Materials Science and Engineering, Insigneo Institute for in silico Medicine, The University of Sheffield, Sheffield S1 3JD, United Kingdom Department of Materials Science and Engineering, Kroto Research Institute, Broad Lane, University of Sheffield, Sheffield S3 7HQ, United KingdomPorous microspheres have the potential for use as injectable bone fillers to obviate the need for open surgery. Successful bone fillers must be able to support vascularisation since tissue engineering scaffolds often cease functioning soon after implantation due to a failure to vascularise rapidly. Here, we test the angiogenic potential of a tissue engineered bone filler based on a photocurable acrylate-based high internal phase emulsion (HIPE). Highly porous microspheres were fabricated via two processes, which were compared. One was taken forward and investigated for its ability to support human mesenchymal progenitor cells and angiogenesis in a chorioallantoic membrane (CAM) assay. Porous microspheres with either a narrow or broad size distribution were prepared via a T-junction microfluidic device or by a controlled stirred-tank reactor of the HIPE water in oil in water (w/o/w), respectively. Culture of human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells showed proliferation over 11 days and formation of cell-microsphere aggregates. In-vitro, hES-MP cells were found to migrate into microspheres through their surface pores over time. The presence of osteoblasts, differentiated from the hES-MP cells, was evidenced through the presence of collagen and calcium after 30 days. Microspheres pre-cultured with cells were implanted into CAM for 7 days and compared with control microspheres without pre-cultured cells. The hES-MP seeded microspheres supported greater angiogenesis, as measured by the number of blood vessels and bifurcations, while the empty scaffolds attracted host chick cell ingrowth. This investigation shows that controlled fabrication of porous microspheres has the potential to create an angiogenic, bone filling material for use as a cell delivery vehicle.http://dx.doi.org/10.1063/1.5008556 |
| spellingShingle | Thomas E. Paterson Giulia Gigliobianco Colin Sherborne Nicola H. Green James M. Dugan Sheila MacNeil Gwendolen C. Reilly Frederik Claeyssens Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis APL Bioengineering |
| title | Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis |
| title_full | Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis |
| title_fullStr | Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis |
| title_full_unstemmed | Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis |
| title_short | Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis |
| title_sort | porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis |
| url | http://dx.doi.org/10.1063/1.5008556 |
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