Characteristics of Ca2+, Mg2+-dependent ATP hydrolysis in sperm cells of infertile men
Disturbances of fertilizing potential of spermatozoa are closely associated with dysfunction of ion-transporting ATPases, in particular Ca2+, Mg2+-АТРase. Reduced activity of tapsigargin-resistant and tapsigargin-sensitive Ca2+, Mg2+-АТРase leads to disruption of Ca2+-homeostasis and is characterist...
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| Format: | Article |
| Language: | English |
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Львівський національний університет імені Івана Франка
2020-05-01
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| Series: | Біологічні студії |
| Subjects: | |
| Online Access: | http://publications.lnu.edu.ua/journals/index.php/biology/article/view/1120 |
| _version_ | 1818690370425323520 |
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| author | O. I. Meskalo R. V. Fafula Z. D. Vorobets |
| author_facet | O. I. Meskalo R. V. Fafula Z. D. Vorobets |
| author_sort | O. I. Meskalo |
| collection | DOAJ |
| description | Disturbances of fertilizing potential of spermatozoa are closely associated with dysfunction of ion-transporting ATPases, in particular Ca2+, Mg2+-АТРase. Reduced activity of tapsigargin-resistant and tapsigargin-sensitive Ca2+, Mg2+-АТРase leads to disruption of Ca2+-homeostasis and is characteristic for abnormal spermatozoa (pathoospermia). In order to study the peculiarities of action of Ca2+, Mg2+-АТРase, we determined the initial reaction rate, the maximum (plateau) amount of the reaction product and the characteristic reaction time. To determine these kinetic parameters of Ca2+, Mg2+-dependent hydrolysis of ATP catalyzed by Ca2+, Mg2+-ATPase, the dynamics of product accumulation of the ATP-hydrolases reaction was studied. The obtained curves were linearized in the coordinates {P/t; P}. Analyzing the changes in the activity of Ca2+, Mg2+-ATPase, the kinetics of primary-active transport of calcium ions through the plasma membrane and membranes of intracellular Ca2+-stores in saponin-permeabilized spermatozoa of infertile men was studied. It was shown that in normozoospermic samples, the transport of Ca2+ ions through the plasma membrane is characterized by a higher capacity than through the membranes of intracellular Ca2+-stores, but it occurs with practically the same initial velocity and characteristic reaction time. It was found that in pathospermic samples, transport of Ca2+ ions with the participation of both components of Ca2+, Mg2+-ATPase occurs less intensively and is characterized by a lower capacity compared to spermatozoa of men with preserved fertility. Specific changes in the kinetic parameters of Ca2+, Mg2+-dependent hydrolysis of ATP lead to inhibition of tapsigargin-resistant and tapsigargin-sensitive Ca2+, Mg2+-ATPase activity and cause a decrease in fertilizing potential of spermatozoa. |
| first_indexed | 2024-12-17T12:24:55Z |
| format | Article |
| id | doaj.art-7ded14b5bd3347b7881047f601b763cf |
| institution | Directory Open Access Journal |
| issn | 1996-4536 2311-0783 |
| language | English |
| last_indexed | 2024-12-17T12:24:55Z |
| publishDate | 2020-05-01 |
| publisher | Львівський національний університет імені Івана Франка |
| record_format | Article |
| series | Біологічні студії |
| spelling | doaj.art-7ded14b5bd3347b7881047f601b763cf2022-12-21T21:48:49ZengЛьвівський національний університет імені Івана ФранкаБіологічні студії1996-45362311-07832020-05-01141334010.30970/sbi.1401.611Characteristics of Ca2+, Mg2+-dependent ATP hydrolysis in sperm cells of infertile menO. I. Meskalo0https://orcid.org/0000-0002-5293-9058R. V. Fafula1https://orcid.org/0000-0002-0121-9093Z. D. Vorobets2https://orcid.org/0000-0001-6016-0186Danylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineDisturbances of fertilizing potential of spermatozoa are closely associated with dysfunction of ion-transporting ATPases, in particular Ca2+, Mg2+-АТРase. Reduced activity of tapsigargin-resistant and tapsigargin-sensitive Ca2+, Mg2+-АТРase leads to disruption of Ca2+-homeostasis and is characteristic for abnormal spermatozoa (pathoospermia). In order to study the peculiarities of action of Ca2+, Mg2+-АТРase, we determined the initial reaction rate, the maximum (plateau) amount of the reaction product and the characteristic reaction time. To determine these kinetic parameters of Ca2+, Mg2+-dependent hydrolysis of ATP catalyzed by Ca2+, Mg2+-ATPase, the dynamics of product accumulation of the ATP-hydrolases reaction was studied. The obtained curves were linearized in the coordinates {P/t; P}. Analyzing the changes in the activity of Ca2+, Mg2+-ATPase, the kinetics of primary-active transport of calcium ions through the plasma membrane and membranes of intracellular Ca2+-stores in saponin-permeabilized spermatozoa of infertile men was studied. It was shown that in normozoospermic samples, the transport of Ca2+ ions through the plasma membrane is characterized by a higher capacity than through the membranes of intracellular Ca2+-stores, but it occurs with practically the same initial velocity and characteristic reaction time. It was found that in pathospermic samples, transport of Ca2+ ions with the participation of both components of Ca2+, Mg2+-ATPase occurs less intensively and is characterized by a lower capacity compared to spermatozoa of men with preserved fertility. Specific changes in the kinetic parameters of Ca2+, Mg2+-dependent hydrolysis of ATP lead to inhibition of tapsigargin-resistant and tapsigargin-sensitive Ca2+, Mg2+-ATPase activity and cause a decrease in fertilizing potential of spermatozoa.http://publications.lnu.edu.ua/journals/index.php/biology/article/view/1120ca2+mg2+-atpaseatp hydrolysisspermatozoamale infertilitypathospermia |
| spellingShingle | O. I. Meskalo R. V. Fafula Z. D. Vorobets Characteristics of Ca2+, Mg2+-dependent ATP hydrolysis in sperm cells of infertile men Біологічні студії ca2+ mg2+-atpase atp hydrolysis spermatozoa male infertility pathospermia |
| title | Characteristics of Ca2+, Mg2+-dependent ATP hydrolysis in sperm cells of infertile men |
| title_full | Characteristics of Ca2+, Mg2+-dependent ATP hydrolysis in sperm cells of infertile men |
| title_fullStr | Characteristics of Ca2+, Mg2+-dependent ATP hydrolysis in sperm cells of infertile men |
| title_full_unstemmed | Characteristics of Ca2+, Mg2+-dependent ATP hydrolysis in sperm cells of infertile men |
| title_short | Characteristics of Ca2+, Mg2+-dependent ATP hydrolysis in sperm cells of infertile men |
| title_sort | characteristics of ca2 mg2 dependent atp hydrolysis in sperm cells of infertile men |
| topic | ca2+ mg2+-atpase atp hydrolysis spermatozoa male infertility pathospermia |
| url | http://publications.lnu.edu.ua/journals/index.php/biology/article/view/1120 |
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