SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus
White strains of Hypsizygus marmoreus are more difficult to cultivate than are brown strains; therefore, new white strain breeding strategies are required. Accordingly, we constructed the genetic map of H. marmoreus with 1996 SNP markers on 11 linkage groups (LGs) spanning 1380.49 cM. Prior to analy...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-12-01
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| Series: | Mycobiology |
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| Online Access: | http://dx.doi.org/10.1080/12298093.2021.2018784 |
| _version_ | 1828145545088073728 |
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| author | Youn-Lee Oh In-Geol Choi Kab-Yeul Jang Min-Seek Kim Min ji Oh Ji-Hoon Im |
| author_facet | Youn-Lee Oh In-Geol Choi Kab-Yeul Jang Min-Seek Kim Min ji Oh Ji-Hoon Im |
| author_sort | Youn-Lee Oh |
| collection | DOAJ |
| description | White strains of Hypsizygus marmoreus are more difficult to cultivate than are brown strains; therefore, new white strain breeding strategies are required. Accordingly, we constructed the genetic map of H. marmoreus with 1996 SNP markers on 11 linkage groups (LGs) spanning 1380.49 cM. Prior to analysis, 82 backcrossed strains (HM8 lines) were generated by mating between KMCC03106-31 and the progenies of the F1 hybrid (Hami-18 × KMCC03106-93). Using HM8, the first 23 quantitative trait loci (QTLs) of yield-related traits were detected with high limit of detection (LOD) scores (1.98–9.86). The length, thickness, and hardness of the stipe were colocated on LG 1. Especially, length of stipe and thickness of stipe were highly correlated given that the correlation coefficients were negative (−0.39, p value ≤ .01). And a typical biomodal distribution was observed for lightness of the pileus and the lightness of the pileus trait belonged to the LG 8, as did traits of earliness and mycelial growth in potato dextrose agar (PDA) medium. Therefore, results for color traits can be suggested that color is controlled by a multi-gene of one locus. The yield trait was highly negatively correlated with the traits for thickness of the stipe (−0.45, p value ≤ .01). Based on additive effects, the white strain was confirmed as recessive; however, traits of mycelial growth, lightness, and quality were inherited by backcrossed HM8 lines. This new genetic map, finely mapped QTLs, and the strong selection markers could be used in molecular breeding of H. marmoreus. |
| first_indexed | 2024-04-11T20:33:39Z |
| format | Article |
| id | doaj.art-7dede59e9d214063951d37a5ede59e6c |
| institution | Directory Open Access Journal |
| issn | 1229-8093 2092-9323 |
| language | English |
| last_indexed | 2024-04-11T20:33:39Z |
| publishDate | 2021-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj.art-7dede59e9d214063951d37a5ede59e6c2022-12-22T04:04:26ZengTaylor & Francis GroupMycobiology1229-80932092-93232021-12-010011010.1080/12298093.2021.20187842018784SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreusYoun-Lee Oh0In-Geol Choi1Kab-Yeul Jang2Min-Seek Kim3Min ji Oh4Ji-Hoon Im5Mushroom Science Division, National Institute of Horticultural and Herbal Science, Rural Development AdministrationDepartment of Biotechnology, College of Life Science and Biotechnology, Korea UniversityMushroom Science Division, National Institute of Horticultural and Herbal Science, Rural Development AdministrationMushroom Science Division, National Institute of Horticultural and Herbal Science, Rural Development AdministrationMushroom Science Division, National Institute of Horticultural and Herbal Science, Rural Development AdministrationMushroom Science Division, National Institute of Horticultural and Herbal Science, Rural Development AdministrationWhite strains of Hypsizygus marmoreus are more difficult to cultivate than are brown strains; therefore, new white strain breeding strategies are required. Accordingly, we constructed the genetic map of H. marmoreus with 1996 SNP markers on 11 linkage groups (LGs) spanning 1380.49 cM. Prior to analysis, 82 backcrossed strains (HM8 lines) were generated by mating between KMCC03106-31 and the progenies of the F1 hybrid (Hami-18 × KMCC03106-93). Using HM8, the first 23 quantitative trait loci (QTLs) of yield-related traits were detected with high limit of detection (LOD) scores (1.98–9.86). The length, thickness, and hardness of the stipe were colocated on LG 1. Especially, length of stipe and thickness of stipe were highly correlated given that the correlation coefficients were negative (−0.39, p value ≤ .01). And a typical biomodal distribution was observed for lightness of the pileus and the lightness of the pileus trait belonged to the LG 8, as did traits of earliness and mycelial growth in potato dextrose agar (PDA) medium. Therefore, results for color traits can be suggested that color is controlled by a multi-gene of one locus. The yield trait was highly negatively correlated with the traits for thickness of the stipe (−0.45, p value ≤ .01). Based on additive effects, the white strain was confirmed as recessive; however, traits of mycelial growth, lightness, and quality were inherited by backcrossed HM8 lines. This new genetic map, finely mapped QTLs, and the strong selection markers could be used in molecular breeding of H. marmoreus.http://dx.doi.org/10.1080/12298093.2021.2018784hypsizygus marmoreus; backcrossingsnpgenetic linkage mapqtl |
| spellingShingle | Youn-Lee Oh In-Geol Choi Kab-Yeul Jang Min-Seek Kim Min ji Oh Ji-Hoon Im SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus Mycobiology hypsizygus marmoreus; backcrossing snp genetic linkage map qtl |
| title | SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus |
| title_full | SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus |
| title_fullStr | SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus |
| title_full_unstemmed | SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus |
| title_short | SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus |
| title_sort | snp based genetic linkage map and quantitative trait locus mapping associated with the agronomically important traits of hypsizygus marmoreus |
| topic | hypsizygus marmoreus; backcrossing snp genetic linkage map qtl |
| url | http://dx.doi.org/10.1080/12298093.2021.2018784 |
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