Protective Effect of Daidzein against Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Male Rats
Hepatocellular carcinoma (HCC) is the second-largest cause of death among all cancer types. Many drugs have been used to treat the disease for a long time but have been mostly discontinued because of their side effects or the development of resistance in the patients with HCC. The administration of...
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MDPI AG
2023-08-01
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| author | Samir A. E. Bashandy Hossam Ebaid Jameel Al-Tamimi Iftekhar Hassan Enayat A. Omara Marawan A. Elbaset Ibrahim M. Alhazza Jamal A. Siddique |
| author_facet | Samir A. E. Bashandy Hossam Ebaid Jameel Al-Tamimi Iftekhar Hassan Enayat A. Omara Marawan A. Elbaset Ibrahim M. Alhazza Jamal A. Siddique |
| author_sort | Samir A. E. Bashandy |
| collection | DOAJ |
| description | Hepatocellular carcinoma (HCC) is the second-largest cause of death among all cancer types. Many drugs have been used to treat the disease for a long time but have been mostly discontinued because of their side effects or the development of resistance in the patients with HCC. The administration of DZ orally is a great focus to address the clinical crisis. Daidzein (DZ) is a prominent isoflavone polyphenolic chemical found in soybeans and other leguminous plants. It has various pharmacological effects, including anti-inflammatory, antihemolytic, and antioxidant. This present study investigates the protective effect of DZ on chemically induced HCC in rat models. The DZ was administered orally four weeks before HCC induction and continued during treatment. Our study included four treatment groups: control (group 1, without any treatment), HCC-induced rats (group II), an HCC group treated with DZ at 20 mg/kg (group III), and an HCC group treated with DZ at 40 mg/kg (group IV). HCC rats showed elevation in all the HCC markers (AFP, GPC3, and VEGF), liver function markers (ALP, ALT, and AST), inflammatory markers (IL-6, TNF-α, and CRP), and lipid markers concomitant with a decrease in antioxidant enzymes and protein. However, groups III and IV demonstrated dose-dependent alleviation in the previous parameters resulting from HCC. In addition, the high dose of DZ reduces many hepatological changes in HCC rats. All study parameters improved with DZ administration. Due to its antioxidant and anti-inflammatory characteristics, DZ is a promising HCC treatment option for clinical use. |
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| series | Biology |
| spelling | doaj.art-7defc711d2a442b2b2669619125b05ea2023-11-19T09:38:12ZengMDPI AGBiology2079-77372023-08-01129118410.3390/biology12091184Protective Effect of Daidzein against Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Male RatsSamir A. E. Bashandy0Hossam Ebaid1Jameel Al-Tamimi2Iftekhar Hassan3Enayat A. Omara4Marawan A. Elbaset5Ibrahim M. Alhazza6Jamal A. Siddique7Pharmacology Department, National Research Centre, 33 El-Bohouth St., Dokki, Cairo 12622, EgyptZoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaZoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaZoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaPathology Department, National Research Centre, 33 El-Bohouth St., Dokki, Cairo 12622, EgyptPharmacology Department, National Research Centre, 33 El-Bohouth St., Dokki, Cairo 12622, EgyptZoology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Materials Engineering and Chemistry, Faculty of Civil Engineering, Czech Technical University (CVUT), Praha 6, 16629 Prague, Czech RepublicHepatocellular carcinoma (HCC) is the second-largest cause of death among all cancer types. Many drugs have been used to treat the disease for a long time but have been mostly discontinued because of their side effects or the development of resistance in the patients with HCC. The administration of DZ orally is a great focus to address the clinical crisis. Daidzein (DZ) is a prominent isoflavone polyphenolic chemical found in soybeans and other leguminous plants. It has various pharmacological effects, including anti-inflammatory, antihemolytic, and antioxidant. This present study investigates the protective effect of DZ on chemically induced HCC in rat models. The DZ was administered orally four weeks before HCC induction and continued during treatment. Our study included four treatment groups: control (group 1, without any treatment), HCC-induced rats (group II), an HCC group treated with DZ at 20 mg/kg (group III), and an HCC group treated with DZ at 40 mg/kg (group IV). HCC rats showed elevation in all the HCC markers (AFP, GPC3, and VEGF), liver function markers (ALP, ALT, and AST), inflammatory markers (IL-6, TNF-α, and CRP), and lipid markers concomitant with a decrease in antioxidant enzymes and protein. However, groups III and IV demonstrated dose-dependent alleviation in the previous parameters resulting from HCC. In addition, the high dose of DZ reduces many hepatological changes in HCC rats. All study parameters improved with DZ administration. Due to its antioxidant and anti-inflammatory characteristics, DZ is a promising HCC treatment option for clinical use.https://www.mdpi.com/2079-7737/12/9/1184hepatocellular carcinomaDaidzeinCCL4inflammatory markers |
| spellingShingle | Samir A. E. Bashandy Hossam Ebaid Jameel Al-Tamimi Iftekhar Hassan Enayat A. Omara Marawan A. Elbaset Ibrahim M. Alhazza Jamal A. Siddique Protective Effect of Daidzein against Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Male Rats Biology hepatocellular carcinoma Daidzein CCL4 inflammatory markers |
| title | Protective Effect of Daidzein against Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Male Rats |
| title_full | Protective Effect of Daidzein against Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Male Rats |
| title_fullStr | Protective Effect of Daidzein against Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Male Rats |
| title_full_unstemmed | Protective Effect of Daidzein against Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Male Rats |
| title_short | Protective Effect of Daidzein against Diethylnitrosamine/Carbon Tetrachloride-Induced Hepatocellular Carcinoma in Male Rats |
| title_sort | protective effect of daidzein against diethylnitrosamine carbon tetrachloride induced hepatocellular carcinoma in male rats |
| topic | hepatocellular carcinoma Daidzein CCL4 inflammatory markers |
| url | https://www.mdpi.com/2079-7737/12/9/1184 |
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