Tolerance of Immobilized Yeast Cells in Imidazolium-Based Ionic Liquids
Ionic liquids (ILs) are considered as another ’green solvent’, after the supercritical carbon dioxide. They are a promising reaction medium for biocatalysis process. The tolerance of active cells in hydrophobic imidazolium-based ILs (1-R-3-methylimidazolium hexafluorophosphate, [RMim][PF6]) has been...
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| Format: | Article |
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University of Zagreb Faculty of Food Technology and Biotechnology
2009-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/52619 |
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| author | Yu Wang Xian-Ke Li Zao-Sheng Lv Bin Lai Shu-Qin Yang Jian-Guo Liao Rong Zeng Zhong-Hua Yang |
| author_facet | Yu Wang Xian-Ke Li Zao-Sheng Lv Bin Lai Shu-Qin Yang Jian-Guo Liao Rong Zeng Zhong-Hua Yang |
| author_sort | Yu Wang |
| collection | DOAJ |
| description | Ionic liquids (ILs) are considered as another ’green solvent’, after the supercritical carbon dioxide. They are a promising reaction medium for biocatalysis process. The tolerance of active cells in hydrophobic imidazolium-based ILs (1-R-3-methylimidazolium hexafluorophosphate, [RMim][PF6]) has been studied in this work. Calcium-alginate-entrapped baker’s yeast has been chosen as the model of living cells. The results show that this kind of ILs possess a certain degree of biocompatibility. The tolerance of yeast cells to the ILs decreases with the increase of the R chain length of these ILs. The experiment indicated that 1-butyl-3-methylimidazolium hexafluorophosphate ([BMim][PF6]) possessed excellent biocompatibility compared to the other imidazolium-based ILs. The moisture content in the ILs was the key factor that affected the tolerance. The activity retention of yeast cells pretreated with [BMim][PF6] saturated with water and aqueous [BMim][PF6] biphasic system was about 70 %, but it was only 50 % with the anhydrous [BMim][PF6]. Although the yeast cells were pretreated with [BMim][PF6] for 24 h, the activity retention was up to 45 %. The yeast cells had around 50 % activity after being pretreated 4 times with [BMim][PF6]. This shows that the water immiscible ILs possess good biocompatibility, and they are suitable for application as the reaction medium catalyzed by living cells. |
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| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T08:30:50Z |
| publishDate | 2009-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
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| series | Food Technology and Biotechnology |
| spelling | doaj.art-7df2838f192f446ca5fbfec293e26f0d2023-12-02T20:12:52ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062009-01-014716266Tolerance of Immobilized Yeast Cells in Imidazolium-Based Ionic LiquidsYu Wang0Xian-Ke Li1Zao-Sheng Lv2Bin Lai3Shu-Qin Yang4Jian-Guo Liao5Rong Zeng6Zhong-Hua Yang7College of Chemical Engineering and Technology, Wuhan University of Science and Technology, CN-430081 Wuhan, PR ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, CN-430081 Wuhan, PR ChinaCollege of Chemistry and Chemical Engineering, Hubei University, CN-430062 Wuhan, PR ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, CN-430081 Wuhan, PR ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, CN-430081 Wuhan, PR ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, CN-430081 Wuhan, PR ChinaCollege of Chemistry and Chemical Engineering, Hubei University, CN-430062 Wuhan, PR ChinaCollege of Chemical Engineering and Technology, Wuhan University of Science and Technology, CN-430081 Wuhan, PR ChinaIonic liquids (ILs) are considered as another ’green solvent’, after the supercritical carbon dioxide. They are a promising reaction medium for biocatalysis process. The tolerance of active cells in hydrophobic imidazolium-based ILs (1-R-3-methylimidazolium hexafluorophosphate, [RMim][PF6]) has been studied in this work. Calcium-alginate-entrapped baker’s yeast has been chosen as the model of living cells. The results show that this kind of ILs possess a certain degree of biocompatibility. The tolerance of yeast cells to the ILs decreases with the increase of the R chain length of these ILs. The experiment indicated that 1-butyl-3-methylimidazolium hexafluorophosphate ([BMim][PF6]) possessed excellent biocompatibility compared to the other imidazolium-based ILs. The moisture content in the ILs was the key factor that affected the tolerance. The activity retention of yeast cells pretreated with [BMim][PF6] saturated with water and aqueous [BMim][PF6] biphasic system was about 70 %, but it was only 50 % with the anhydrous [BMim][PF6]. Although the yeast cells were pretreated with [BMim][PF6] for 24 h, the activity retention was up to 45 %. The yeast cells had around 50 % activity after being pretreated 4 times with [BMim][PF6]. This shows that the water immiscible ILs possess good biocompatibility, and they are suitable for application as the reaction medium catalyzed by living cells.http://hrcak.srce.hr/file/52619ionic liquidbiocompatibilitycell toxicityyeast cellbiocatalysis |
| spellingShingle | Yu Wang Xian-Ke Li Zao-Sheng Lv Bin Lai Shu-Qin Yang Jian-Guo Liao Rong Zeng Zhong-Hua Yang Tolerance of Immobilized Yeast Cells in Imidazolium-Based Ionic Liquids Food Technology and Biotechnology ionic liquid biocompatibility cell toxicity yeast cell biocatalysis |
| title | Tolerance of Immobilized Yeast Cells in Imidazolium-Based Ionic Liquids |
| title_full | Tolerance of Immobilized Yeast Cells in Imidazolium-Based Ionic Liquids |
| title_fullStr | Tolerance of Immobilized Yeast Cells in Imidazolium-Based Ionic Liquids |
| title_full_unstemmed | Tolerance of Immobilized Yeast Cells in Imidazolium-Based Ionic Liquids |
| title_short | Tolerance of Immobilized Yeast Cells in Imidazolium-Based Ionic Liquids |
| title_sort | tolerance of immobilized yeast cells in imidazolium based ionic liquids |
| topic | ionic liquid biocompatibility cell toxicity yeast cell biocatalysis |
| url | http://hrcak.srce.hr/file/52619 |
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