Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea
The sugar beet cyst nematode, Heterodera schachtii is a well known pathogen on Chinese cabbage in the highland fields of Korea. However, a race of cyst forming nematode with close morphological resemblance to H. trifolii was recently isolated from the same Chinese cabbage fields. Morphological speci...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2018-08-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097818 |
| _version_ | 1828825222920273920 |
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| author | Abraham Okki Mwamula Hyoung-Rai Ko Youngjoon Kim Young Ho Kim Jae-Kook Lee Dong Woon Lee |
| author_facet | Abraham Okki Mwamula Hyoung-Rai Ko Youngjoon Kim Young Ho Kim Jae-Kook Lee Dong Woon Lee |
| author_sort | Abraham Okki Mwamula |
| collection | DOAJ |
| description | The sugar beet cyst nematode, Heterodera schachtii is a well known pathogen on Chinese cabbage in the highland fields of Korea. However, a race of cyst forming nematode with close morphological resemblance to H. trifolii was recently isolated from the same Chinese cabbage fields. Morphological species differentiation between the two cyst nematodes is challenging, with only minor differences between them. Thus, this study described the newly intercepted H. trifolii population, and reviewed morphological and molecular characteristics conceivably essential in differentiating the two nematode species. A comparison of morphometric measurements of both infective juveniles and vulval cones of cysts showed significant differences between the two cyst nematodes. When total RNA and genomic DNA were extracted from a mixed field population, COI genes and ITS regions were clearly amplified with primers of the two Heterodera species, suggesting that Heterodera population collected from the Chinese cabbage field consisted of a mixture of two species. COI and ITS of H. trifolii were predominantly amplified from nucleotides prepared from H. trifolii monoxenic population whereas those of H. schachtii were strongly detected in H. schachtii monoxenic cultures. Thus, this study confirms the coexistence of the two species in some Chinese cabbage fields; and the presence of H. trifolii in Korea is reported here for the first time. |
| first_indexed | 2024-12-12T14:12:01Z |
| format | Article |
| id | doaj.art-7df330bb3f004982b97341ba3043fcae |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-12T14:12:01Z |
| publishDate | 2018-08-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-7df330bb3f004982b97341ba3043fcae2022-12-22T00:22:01ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542018-08-0134429730710.5423/PPJ.OA.12.2017.026210.5423PPJ.OA.12.2017.0262Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in KoreaAbraham Okki Mwamula0Hyoung-Rai Ko1Youngjoon Kim2Young Ho Kim3Jae-Kook Lee4Dong Woon Lee5Department of Ecological Science, Kyungpook National University, Sangju 37224, KoreaCrop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, KoreaDepartment of Ecological Science, Kyungpook National University, Sangju 37224, KoreaDepartment of Ecological Science, Kyungpook National University, Sangju 37224, KoreaCrop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, KoreaDepartment of Ecological Science, Kyungpook National University, Sangju 37224, KoreaThe sugar beet cyst nematode, Heterodera schachtii is a well known pathogen on Chinese cabbage in the highland fields of Korea. However, a race of cyst forming nematode with close morphological resemblance to H. trifolii was recently isolated from the same Chinese cabbage fields. Morphological species differentiation between the two cyst nematodes is challenging, with only minor differences between them. Thus, this study described the newly intercepted H. trifolii population, and reviewed morphological and molecular characteristics conceivably essential in differentiating the two nematode species. A comparison of morphometric measurements of both infective juveniles and vulval cones of cysts showed significant differences between the two cyst nematodes. When total RNA and genomic DNA were extracted from a mixed field population, COI genes and ITS regions were clearly amplified with primers of the two Heterodera species, suggesting that Heterodera population collected from the Chinese cabbage field consisted of a mixture of two species. COI and ITS of H. trifolii were predominantly amplified from nucleotides prepared from H. trifolii monoxenic population whereas those of H. schachtii were strongly detected in H. schachtii monoxenic cultures. Thus, this study confirms the coexistence of the two species in some Chinese cabbage fields; and the presence of H. trifolii in Korea is reported here for the first time.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097818infective juvenilemorphometricsvulval cone |
| spellingShingle | Abraham Okki Mwamula Hyoung-Rai Ko Youngjoon Kim Young Ho Kim Jae-Kook Lee Dong Woon Lee Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea The Plant Pathology Journal infective juvenile morphometrics vulval cone |
| title | Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea |
| title_full | Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea |
| title_fullStr | Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea |
| title_full_unstemmed | Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea |
| title_short | Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea |
| title_sort | morphological and molecular characterization of heterodera schachtii and the newly recorded cyst nematode h trifolii associated with chinese cabbage in korea |
| topic | infective juvenile morphometrics vulval cone |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097818 |
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