Ultrastructural Characterization of Membrane Rearrangements Induced by Porcine Epidemic Diarrhea Virus Infection
The porcine epidemic diarrhea virus (PEDV) is a coronavirus (CoV) belonging to the α-CoV genus and it causes high mortality in infected sucking piglets, resulting in substantial losses in the farming industry. CoV trigger a drastic reorganization of host cell membranes to promote their replication a...
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MDPI AG
2017-09-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/9/9/251 |
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| author | Xingdong Zhou Yingying Cong Tineke Veenendaal Judith Klumperman Dongfang Shi Muriel Mari Fulvio Reggiori |
| author_facet | Xingdong Zhou Yingying Cong Tineke Veenendaal Judith Klumperman Dongfang Shi Muriel Mari Fulvio Reggiori |
| author_sort | Xingdong Zhou |
| collection | DOAJ |
| description | The porcine epidemic diarrhea virus (PEDV) is a coronavirus (CoV) belonging to the α-CoV genus and it causes high mortality in infected sucking piglets, resulting in substantial losses in the farming industry. CoV trigger a drastic reorganization of host cell membranes to promote their replication and egression, but a detailed description of the intracellular remodeling induced by PEDV is still missing. In this study, we examined qualitatively and quantitatively, using electron microscopy, the intracellular membrane reorganization induced by PEDV over the course of an infection. With our ultrastructural approach, we reveal that, as most of CoV, PEDV initially forms double-membrane vesicles (DMVs) and convoluted membranes (CMs), which probably serve as replication/transcription platforms. Interestingly, we also found that viral particles start to form almost simultaneously in both the endoplasmic reticulum and the large virion-containing vacuoles (LVCVs), which are compartments originating from the Golgi, confirming that α-CoV assemble indistinguishably in two different organelles of the secretory pathway. Moreover, PEDV virons appear to have an immature and a mature form, similar to another α-CoV the transmissible gastroenteritis coronavirus (TGEV). Altogether, our study underlies the similarities and differences between the lifecycle of α-CoV and that of viruses belonging to other CoV subfamilies. |
| first_indexed | 2024-12-10T03:54:35Z |
| format | Article |
| id | doaj.art-7df5e9877f164250a9e719de71ed8758 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-10T03:54:35Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-7df5e9877f164250a9e719de71ed87582022-12-22T02:03:09ZengMDPI AGViruses1999-49152017-09-019925110.3390/v9090251v9090251Ultrastructural Characterization of Membrane Rearrangements Induced by Porcine Epidemic Diarrhea Virus InfectionXingdong Zhou0Yingying Cong1Tineke Veenendaal2Judith Klumperman3Dongfang Shi4Muriel Mari5Fulvio Reggiori6Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaDepartment of Cell Biology, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsDepartment of Cell Biology, Center for Molecular Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The NetherlandsDepartment of Cell Biology, Center for Molecular Medicine, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The NetherlandsDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaDepartment of Cell Biology, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsDepartment of Cell Biology, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, 9713 AV Groningen, The NetherlandsThe porcine epidemic diarrhea virus (PEDV) is a coronavirus (CoV) belonging to the α-CoV genus and it causes high mortality in infected sucking piglets, resulting in substantial losses in the farming industry. CoV trigger a drastic reorganization of host cell membranes to promote their replication and egression, but a detailed description of the intracellular remodeling induced by PEDV is still missing. In this study, we examined qualitatively and quantitatively, using electron microscopy, the intracellular membrane reorganization induced by PEDV over the course of an infection. With our ultrastructural approach, we reveal that, as most of CoV, PEDV initially forms double-membrane vesicles (DMVs) and convoluted membranes (CMs), which probably serve as replication/transcription platforms. Interestingly, we also found that viral particles start to form almost simultaneously in both the endoplasmic reticulum and the large virion-containing vacuoles (LVCVs), which are compartments originating from the Golgi, confirming that α-CoV assemble indistinguishably in two different organelles of the secretory pathway. Moreover, PEDV virons appear to have an immature and a mature form, similar to another α-CoV the transmissible gastroenteritis coronavirus (TGEV). Altogether, our study underlies the similarities and differences between the lifecycle of α-CoV and that of viruses belonging to other CoV subfamilies.https://www.mdpi.com/1999-4915/9/9/251PEDValpha-coronaviruslifecycleelectron microscopymembrane rearrangement |
| spellingShingle | Xingdong Zhou Yingying Cong Tineke Veenendaal Judith Klumperman Dongfang Shi Muriel Mari Fulvio Reggiori Ultrastructural Characterization of Membrane Rearrangements Induced by Porcine Epidemic Diarrhea Virus Infection Viruses PEDV alpha-coronavirus lifecycle electron microscopy membrane rearrangement |
| title | Ultrastructural Characterization of Membrane Rearrangements Induced by Porcine Epidemic Diarrhea Virus Infection |
| title_full | Ultrastructural Characterization of Membrane Rearrangements Induced by Porcine Epidemic Diarrhea Virus Infection |
| title_fullStr | Ultrastructural Characterization of Membrane Rearrangements Induced by Porcine Epidemic Diarrhea Virus Infection |
| title_full_unstemmed | Ultrastructural Characterization of Membrane Rearrangements Induced by Porcine Epidemic Diarrhea Virus Infection |
| title_short | Ultrastructural Characterization of Membrane Rearrangements Induced by Porcine Epidemic Diarrhea Virus Infection |
| title_sort | ultrastructural characterization of membrane rearrangements induced by porcine epidemic diarrhea virus infection |
| topic | PEDV alpha-coronavirus lifecycle electron microscopy membrane rearrangement |
| url | https://www.mdpi.com/1999-4915/9/9/251 |
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