Cryopreservation protocol for equine platelet-rich plasma
In this preliminary study, a new equine platelet-rich plasma (PRP) cryopreservation protocol was evaluated. PRP was obtained by a double centrifugation technique of whole blood collected from 8 adult healthy ponies. A fresh sample of PRP was analyzed for total platelet count, mean platelet volume (M...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2016-06-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21777 |
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| author | Liomara Andressa do Amaral Kwirant Flavio Desessards De La Côrte Karin Erica Brass Mara Iolanda Batistella Rubin Raqueli Teresinha França Patrícia Soares Vieira Mariana Cocco |
| author_facet | Liomara Andressa do Amaral Kwirant Flavio Desessards De La Côrte Karin Erica Brass Mara Iolanda Batistella Rubin Raqueli Teresinha França Patrícia Soares Vieira Mariana Cocco |
| author_sort | Liomara Andressa do Amaral Kwirant |
| collection | DOAJ |
| description | In this preliminary study, a new equine platelet-rich plasma (PRP) cryopreservation protocol was evaluated. PRP was obtained by a double centrifugation technique of whole blood collected from 8 adult healthy ponies. A fresh sample of PRP was analyzed for total platelet count, mean platelet volume (MPV), and platelet morphology. Upon morphological evaluation, 200 platelets were counted using a differential interference contrast microscope with a 40x phase objective and classified as activated (with pseudopodia), inactivated (normal discoid shape), or uncertain state (spherical shape, without pseudopodia). Two other PRP samples, one containing DMSO as a cryoprotectant and the other without DMSO, were stored in a mechanical freezer at –80ºC. After 14 days, the frozen samples were thawed and submitted to the same analysis as described above. The fresh PRP showed a platelet count of 830 (±95.3) x103 uL-1, an MPV of 5.2 (±0.07) fL, and composed of 4% activated platelets. There was no significant difference in platelet count, MPV, and activated platelets between fresh and 6% DMSO frozen PRP samples (617.9±65.5x103uL-1; 5.3±0.06fL; 9.5%) (p > 0.05). On the other hand, samples frozen without DMSO showed a significantly lower platelet count (519.6±66.1x103uL-1), higher MPV (5.7±0.08fL), and more activated platelets (13.9%) than the other groups (p < 0.05). The 6% DMSO was able to preserve platelet morphology in PRP stored at –80oC for 14 days, but studies on platelet function of thawed PRP are still needed. |
| first_indexed | 2024-12-12T00:55:17Z |
| format | Article |
| id | doaj.art-7df6a292ad214969aeea862e7bb6de4f |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-12T00:55:17Z |
| publishDate | 2016-06-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-7df6a292ad214969aeea862e7bb6de4f2022-12-22T00:43:54ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592016-06-013731389139610.5433/1679-0359.2016v37n3p138913981Cryopreservation protocol for equine platelet-rich plasmaLiomara Andressa do Amaral Kwirant0Flavio Desessards De La Côrte1Karin Erica Brass2Mara Iolanda Batistella Rubin3Raqueli Teresinha França4Patrícia Soares Vieira5Mariana Cocco6Universidade Federal do Rio Grande do SulUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de PelotasUniversidade Federal de Santa MariaIn this preliminary study, a new equine platelet-rich plasma (PRP) cryopreservation protocol was evaluated. PRP was obtained by a double centrifugation technique of whole blood collected from 8 adult healthy ponies. A fresh sample of PRP was analyzed for total platelet count, mean platelet volume (MPV), and platelet morphology. Upon morphological evaluation, 200 platelets were counted using a differential interference contrast microscope with a 40x phase objective and classified as activated (with pseudopodia), inactivated (normal discoid shape), or uncertain state (spherical shape, without pseudopodia). Two other PRP samples, one containing DMSO as a cryoprotectant and the other without DMSO, were stored in a mechanical freezer at –80ºC. After 14 days, the frozen samples were thawed and submitted to the same analysis as described above. The fresh PRP showed a platelet count of 830 (±95.3) x103 uL-1, an MPV of 5.2 (±0.07) fL, and composed of 4% activated platelets. There was no significant difference in platelet count, MPV, and activated platelets between fresh and 6% DMSO frozen PRP samples (617.9±65.5x103uL-1; 5.3±0.06fL; 9.5%) (p > 0.05). On the other hand, samples frozen without DMSO showed a significantly lower platelet count (519.6±66.1x103uL-1), higher MPV (5.7±0.08fL), and more activated platelets (13.9%) than the other groups (p < 0.05). The 6% DMSO was able to preserve platelet morphology in PRP stored at –80oC for 14 days, but studies on platelet function of thawed PRP are still needed.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21777PlateletsStorageEquineDmso. |
| spellingShingle | Liomara Andressa do Amaral Kwirant Flavio Desessards De La Côrte Karin Erica Brass Mara Iolanda Batistella Rubin Raqueli Teresinha França Patrícia Soares Vieira Mariana Cocco Cryopreservation protocol for equine platelet-rich plasma Semina: Ciências Agrárias Platelets Storage Equine Dmso. |
| title | Cryopreservation protocol for equine platelet-rich plasma |
| title_full | Cryopreservation protocol for equine platelet-rich plasma |
| title_fullStr | Cryopreservation protocol for equine platelet-rich plasma |
| title_full_unstemmed | Cryopreservation protocol for equine platelet-rich plasma |
| title_short | Cryopreservation protocol for equine platelet-rich plasma |
| title_sort | cryopreservation protocol for equine platelet rich plasma |
| topic | Platelets Storage Equine Dmso. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21777 |
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