H19 may regulate the immune cell infiltration in carcinogenesis of gastric cancer through miR-378a-5p/SERPINH1 signaling
Abstract Background Increasing studies have indicated that noncoding RNA (ncRNA)-mediated competing endogenous RNA (ceRNA) network serves as a significant role in cancer progression, but the underlying regulatory mechanisms of which in gastric cancer (GC) remain largely unclear. Methods Based on Gen...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-022-02760-6 |
| _version_ | 1811267318206955520 |
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| author | Jianxin Li Ting Han Xin Wang Yinchun Wang Xuan Chen Wangsheng Chen Qingqiang Yang |
| author_facet | Jianxin Li Ting Han Xin Wang Yinchun Wang Xuan Chen Wangsheng Chen Qingqiang Yang |
| author_sort | Jianxin Li |
| collection | DOAJ |
| description | Abstract Background Increasing studies have indicated that noncoding RNA (ncRNA)-mediated competing endogenous RNA (ceRNA) network serves as a significant role in cancer progression, but the underlying regulatory mechanisms of which in gastric cancer (GC) remain largely unclear. Methods Based on Gene Expression Omnibus and The Cancer Genome Atlas datasets, potential biomarkers for GC were screened and validated by machine learning. Then, upstream regulatory ncRNA of potential biomarkers was identified to construct a novel ceRNA network in GC through means of stepwise reverse prediction and validation. Ultimately, tumor immune cell infiltration analysis was performed based on the EPIC algorithm. Results A total of 188 differentially expressed genes (DEGs) were screened, and three candidate diagnostic biomarkers (FAP, PSAPL1, and SERPINH1) for GC were identified and validated. Subsequently, H19 and miR-378a-5p were identified as upstream regulatory ncRNAs that could potentially bind SERPINH1 in GC. Moreover, Immune infiltration analysis revealed that each component in the ceRNA network (H19/miR-378a-5p/SERPINH1) was significantly correlated with the infiltration abundances of diverse tumor-infiltrating immune cells. Conclusions H19 may regulate the immune cell infiltration in carcinogenesis of GC through miR-378a-5p/SERPINH1 signaling. |
| first_indexed | 2024-04-12T21:00:31Z |
| format | Article |
| id | doaj.art-7df8e99964334c109062571b1d450449 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-04-12T21:00:31Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-7df8e99964334c109062571b1d4504492022-12-22T03:16:51ZengBMCWorld Journal of Surgical Oncology1477-78192022-09-0120111110.1186/s12957-022-02760-6H19 may regulate the immune cell infiltration in carcinogenesis of gastric cancer through miR-378a-5p/SERPINH1 signalingJianxin Li0Ting Han1Xin Wang2Yinchun Wang3Xuan Chen4Wangsheng Chen5Qingqiang Yang6Department of General Surgery (Gastrointestinal Surgery), The Affiliated Hospital of Southwest Medical UniversityDepartment of General Surgery (Gastrointestinal Surgery), The Affiliated Hospital of Southwest Medical UniversityDepartment of General Surgery (Gastrointestinal Surgery), The Affiliated Hospital of Southwest Medical UniversityDepartment of General Surgery (Gastrointestinal Surgery), The Affiliated Hospital of Southwest Medical UniversityDepartment of General Surgery (Gastrointestinal Surgery), The Affiliated Hospital of Southwest Medical UniversityDepartment of General Surgery (Gastrointestinal Surgery), The Affiliated Hospital of Southwest Medical UniversityDepartment of General Surgery (Gastrointestinal Surgery), The Affiliated Hospital of Southwest Medical UniversityAbstract Background Increasing studies have indicated that noncoding RNA (ncRNA)-mediated competing endogenous RNA (ceRNA) network serves as a significant role in cancer progression, but the underlying regulatory mechanisms of which in gastric cancer (GC) remain largely unclear. Methods Based on Gene Expression Omnibus and The Cancer Genome Atlas datasets, potential biomarkers for GC were screened and validated by machine learning. Then, upstream regulatory ncRNA of potential biomarkers was identified to construct a novel ceRNA network in GC through means of stepwise reverse prediction and validation. Ultimately, tumor immune cell infiltration analysis was performed based on the EPIC algorithm. Results A total of 188 differentially expressed genes (DEGs) were screened, and three candidate diagnostic biomarkers (FAP, PSAPL1, and SERPINH1) for GC were identified and validated. Subsequently, H19 and miR-378a-5p were identified as upstream regulatory ncRNAs that could potentially bind SERPINH1 in GC. Moreover, Immune infiltration analysis revealed that each component in the ceRNA network (H19/miR-378a-5p/SERPINH1) was significantly correlated with the infiltration abundances of diverse tumor-infiltrating immune cells. Conclusions H19 may regulate the immune cell infiltration in carcinogenesis of GC through miR-378a-5p/SERPINH1 signaling.https://doi.org/10.1186/s12957-022-02760-6Gastric cancerNoncoding RNACompeting endogenous RNABiomarkerImmune cell infiltration |
| spellingShingle | Jianxin Li Ting Han Xin Wang Yinchun Wang Xuan Chen Wangsheng Chen Qingqiang Yang H19 may regulate the immune cell infiltration in carcinogenesis of gastric cancer through miR-378a-5p/SERPINH1 signaling World Journal of Surgical Oncology Gastric cancer Noncoding RNA Competing endogenous RNA Biomarker Immune cell infiltration |
| title | H19 may regulate the immune cell infiltration in carcinogenesis of gastric cancer through miR-378a-5p/SERPINH1 signaling |
| title_full | H19 may regulate the immune cell infiltration in carcinogenesis of gastric cancer through miR-378a-5p/SERPINH1 signaling |
| title_fullStr | H19 may regulate the immune cell infiltration in carcinogenesis of gastric cancer through miR-378a-5p/SERPINH1 signaling |
| title_full_unstemmed | H19 may regulate the immune cell infiltration in carcinogenesis of gastric cancer through miR-378a-5p/SERPINH1 signaling |
| title_short | H19 may regulate the immune cell infiltration in carcinogenesis of gastric cancer through miR-378a-5p/SERPINH1 signaling |
| title_sort | h19 may regulate the immune cell infiltration in carcinogenesis of gastric cancer through mir 378a 5p serpinh1 signaling |
| topic | Gastric cancer Noncoding RNA Competing endogenous RNA Biomarker Immune cell infiltration |
| url | https://doi.org/10.1186/s12957-022-02760-6 |
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