Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods
The objective of this study was to compare 2 laying hen strains in 5 production periods regarding phytase activity, phytate (InsP6) degradation, and myo-inositol (MI) release in the digestive tract and phosphorus (P) and calcium (Ca) utilization. One offspring of 10 nonrelated roosters per strain (L...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579120306118 |
| _version_ | 1818715767191896064 |
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| author | Vera Sommerfeld Korinna Huber Jörn Bennewitz Amélia Camarinha-Silva Martin Hasselmann Siriluck Ponsuksili Jana Seifert Volker Stefanski Klaus Wimmers Markus Rodehutscord |
| author_facet | Vera Sommerfeld Korinna Huber Jörn Bennewitz Amélia Camarinha-Silva Martin Hasselmann Siriluck Ponsuksili Jana Seifert Volker Stefanski Klaus Wimmers Markus Rodehutscord |
| author_sort | Vera Sommerfeld |
| collection | DOAJ |
| description | The objective of this study was to compare 2 laying hen strains in 5 production periods regarding phytase activity, phytate (InsP6) degradation, and myo-inositol (MI) release in the digestive tract and phosphorus (P) and calcium (Ca) utilization. One offspring of 10 nonrelated roosters per strain (Lohmann Brown-classic (LB) or Lohmann LSL-classic (LSL)) was placed in one of 20 metabolic units in a completely randomized block design in week 8, 14, 22, 28, and 58 of life. All hens were fed the same corn and soybean meal–based diet at one time, but the diet composition was adjusted to the requirements in the respective period. For 4 consecutive days, excreta were collected quantitatively at 24-hour intervals. In week 10, 16, 24, 30, and 60, the blood plasma, digesta of crop, gizzard, jejunum, ileum, and ceca, and mucosa of the jejunum was collected. The concentration of inorganic P in the blood plasma was higher in LB than in LSL hens (P = 0.026). Plasma Ca concentrations increased with each period (P < 0.001) in both strains. In jejunum digesta, the MI concentration did not differ between strains, but InsP6 concentration was higher in LB than in LSL hens (P = 0.002) and the highest in week 30 and 60. Total phosphatase and phytase activities were higher in LB than in LSL hens (P ≤ 0.009). Period effects were also significant for these enzymes. Concentrations of some constituents of the cecal content were different between the strains. The MI concentration in the egg albumen and yolk was higher in LB than in LSL hens. Differences in InsP6- and MI-related metabolism of the 2 hen strains existed. These differences were partly dependent of the period. Especially, week 24 was a period of remarkable change of metabolism. Great differences also existed among individuals, making it worth to have a closer look at the metabolism of individuals in addition to evaluating treatment means. Further studies on metabolic, genetic, and microbiome level may help explain these differences. |
| first_indexed | 2024-12-17T19:08:35Z |
| format | Article |
| id | doaj.art-7df9fd77081f4eb9890b66f1f49873bc |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-17T19:08:35Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-7df9fd77081f4eb9890b66f1f49873bc2022-12-21T21:35:55ZengElsevierPoultry Science0032-57912020-12-01991267976808Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periodsVera Sommerfeld0Korinna Huber1Jörn Bennewitz2Amélia Camarinha-Silva3Martin Hasselmann4Siriluck Ponsuksili5Jana Seifert6Volker Stefanski7Klaus Wimmers8Markus Rodehutscord9Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Animal Science, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Animal Science, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Animal Science, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Animal Science, University of Hohenheim, 70599 Stuttgart, GermanyLeibniz Institute for Farm Animal Biology, Institute for Genome Biology, 18196 Dummerstorf, GermanyInstitute of Animal Science, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Animal Science, University of Hohenheim, 70599 Stuttgart, GermanyLeibniz Institute for Farm Animal Biology, Institute for Genome Biology, 18196 Dummerstorf, GermanyInstitute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany; Corresponding author:The objective of this study was to compare 2 laying hen strains in 5 production periods regarding phytase activity, phytate (InsP6) degradation, and myo-inositol (MI) release in the digestive tract and phosphorus (P) and calcium (Ca) utilization. One offspring of 10 nonrelated roosters per strain (Lohmann Brown-classic (LB) or Lohmann LSL-classic (LSL)) was placed in one of 20 metabolic units in a completely randomized block design in week 8, 14, 22, 28, and 58 of life. All hens were fed the same corn and soybean meal–based diet at one time, but the diet composition was adjusted to the requirements in the respective period. For 4 consecutive days, excreta were collected quantitatively at 24-hour intervals. In week 10, 16, 24, 30, and 60, the blood plasma, digesta of crop, gizzard, jejunum, ileum, and ceca, and mucosa of the jejunum was collected. The concentration of inorganic P in the blood plasma was higher in LB than in LSL hens (P = 0.026). Plasma Ca concentrations increased with each period (P < 0.001) in both strains. In jejunum digesta, the MI concentration did not differ between strains, but InsP6 concentration was higher in LB than in LSL hens (P = 0.002) and the highest in week 30 and 60. Total phosphatase and phytase activities were higher in LB than in LSL hens (P ≤ 0.009). Period effects were also significant for these enzymes. Concentrations of some constituents of the cecal content were different between the strains. The MI concentration in the egg albumen and yolk was higher in LB than in LSL hens. Differences in InsP6- and MI-related metabolism of the 2 hen strains existed. These differences were partly dependent of the period. Especially, week 24 was a period of remarkable change of metabolism. Great differences also existed among individuals, making it worth to have a closer look at the metabolism of individuals in addition to evaluating treatment means. Further studies on metabolic, genetic, and microbiome level may help explain these differences.http://www.sciencedirect.com/science/article/pii/S0032579120306118laying henmucosal phytasemyo-inositolphosphorusphytate degradation |
| spellingShingle | Vera Sommerfeld Korinna Huber Jörn Bennewitz Amélia Camarinha-Silva Martin Hasselmann Siriluck Ponsuksili Jana Seifert Volker Stefanski Klaus Wimmers Markus Rodehutscord Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods Poultry Science laying hen mucosal phytase myo-inositol phosphorus phytate degradation |
| title | Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods |
| title_full | Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods |
| title_fullStr | Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods |
| title_full_unstemmed | Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods |
| title_short | Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods |
| title_sort | phytate degradation myo inositol release and utilization of phosphorus and calcium by two strains of laying hens in five production periods |
| topic | laying hen mucosal phytase myo-inositol phosphorus phytate degradation |
| url | http://www.sciencedirect.com/science/article/pii/S0032579120306118 |
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