The First Step of <i>Neurospora crassa</i> Molybdenum Cofactor Biosynthesis: Regulatory Aspects under N-Derepressing and Nitrate-Inducing Conditions
Molybdenum cofactor (Moco) is the active site prosthetic group found in all Moco dependent enzymes, except for nitrogenase. Mo-enzymes are crucial for viability throughout all kingdoms of life as they catalyze a diverse set of two electron transfer reactions. The highly conserved Moco biosynthesis p...
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MDPI AG
2020-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/4/534 |
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| author | Simon Wajmann Thomas W. Hercher Sabine Buchmeier Robert Hänsch Ralf R. Mendel Tobias Kruse |
| author_facet | Simon Wajmann Thomas W. Hercher Sabine Buchmeier Robert Hänsch Ralf R. Mendel Tobias Kruse |
| author_sort | Simon Wajmann |
| collection | DOAJ |
| description | Molybdenum cofactor (Moco) is the active site prosthetic group found in all Moco dependent enzymes, except for nitrogenase. Mo-enzymes are crucial for viability throughout all kingdoms of life as they catalyze a diverse set of two electron transfer reactions. The highly conserved Moco biosynthesis pathway consists of four different steps in which guanosine triphosphate is converted into cyclic pyranopterin monophosphate, molybdopterin (MPT), and subsequently adenylated MPT and Moco. Although the enzymes and mechanisms involved in these steps are well characterized, the regulation of eukaryotic Moco biosynthesis is not. Within this work, we described the regulation of Moco biosynthesis in the filamentous fungus <i>Neurospora crassa,</i> which revealed the first step of the multi-step pathway to be under transcriptional control. We found, that upon the induction of high cellular Moco demand a single transcript variant of the <i>nit-7</i> gene is increasingly formed pointing towards, that essentially the encoded enzyme NIT7-A is the key player for Moco biosynthesis activity in <i>Neurospora</i>. |
| first_indexed | 2024-03-10T20:36:52Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T20:36:52Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7dfcb29c4347440e9349845941fc22fb2023-11-19T20:57:42ZengMDPI AGMicroorganisms2076-26072020-04-018453410.3390/microorganisms8040534The First Step of <i>Neurospora crassa</i> Molybdenum Cofactor Biosynthesis: Regulatory Aspects under N-Derepressing and Nitrate-Inducing ConditionsSimon Wajmann0Thomas W. Hercher1Sabine Buchmeier2Robert Hänsch3Ralf R. Mendel4Tobias Kruse5TU Braunschweig, Institute of Plant Biology, 38106 Braunschweig, GermanyTU Braunschweig, Institute of Plant Biology, 38106 Braunschweig, GermanyTU Braunschweig, Institute of Physical and Theoretical Chemistry, Antibody Facility, 38106 Braunschweig, GermanyTU Braunschweig, Institute of Plant Biology, 38106 Braunschweig, GermanyTU Braunschweig, Institute of Plant Biology, 38106 Braunschweig, GermanyTU Braunschweig, Institute of Plant Biology, 38106 Braunschweig, GermanyMolybdenum cofactor (Moco) is the active site prosthetic group found in all Moco dependent enzymes, except for nitrogenase. Mo-enzymes are crucial for viability throughout all kingdoms of life as they catalyze a diverse set of two electron transfer reactions. The highly conserved Moco biosynthesis pathway consists of four different steps in which guanosine triphosphate is converted into cyclic pyranopterin monophosphate, molybdopterin (MPT), and subsequently adenylated MPT and Moco. Although the enzymes and mechanisms involved in these steps are well characterized, the regulation of eukaryotic Moco biosynthesis is not. Within this work, we described the regulation of Moco biosynthesis in the filamentous fungus <i>Neurospora crassa,</i> which revealed the first step of the multi-step pathway to be under transcriptional control. We found, that upon the induction of high cellular Moco demand a single transcript variant of the <i>nit-7</i> gene is increasingly formed pointing towards, that essentially the encoded enzyme NIT7-A is the key player for Moco biosynthesis activity in <i>Neurospora</i>.https://www.mdpi.com/2076-2607/8/4/534molybdenum cofactormolybdenum cofactor biosynthesisregulation<i>Neurospora crassa</i> |
| spellingShingle | Simon Wajmann Thomas W. Hercher Sabine Buchmeier Robert Hänsch Ralf R. Mendel Tobias Kruse The First Step of <i>Neurospora crassa</i> Molybdenum Cofactor Biosynthesis: Regulatory Aspects under N-Derepressing and Nitrate-Inducing Conditions Microorganisms molybdenum cofactor molybdenum cofactor biosynthesis regulation <i>Neurospora crassa</i> |
| title | The First Step of <i>Neurospora crassa</i> Molybdenum Cofactor Biosynthesis: Regulatory Aspects under N-Derepressing and Nitrate-Inducing Conditions |
| title_full | The First Step of <i>Neurospora crassa</i> Molybdenum Cofactor Biosynthesis: Regulatory Aspects under N-Derepressing and Nitrate-Inducing Conditions |
| title_fullStr | The First Step of <i>Neurospora crassa</i> Molybdenum Cofactor Biosynthesis: Regulatory Aspects under N-Derepressing and Nitrate-Inducing Conditions |
| title_full_unstemmed | The First Step of <i>Neurospora crassa</i> Molybdenum Cofactor Biosynthesis: Regulatory Aspects under N-Derepressing and Nitrate-Inducing Conditions |
| title_short | The First Step of <i>Neurospora crassa</i> Molybdenum Cofactor Biosynthesis: Regulatory Aspects under N-Derepressing and Nitrate-Inducing Conditions |
| title_sort | first step of i neurospora crassa i molybdenum cofactor biosynthesis regulatory aspects under n derepressing and nitrate inducing conditions |
| topic | molybdenum cofactor molybdenum cofactor biosynthesis regulation <i>Neurospora crassa</i> |
| url | https://www.mdpi.com/2076-2607/8/4/534 |
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