High-speed camera recordings uncover previously unidentified elements of zebrafish mating behaviors integral to successful fertilization
Abstract The mating behavior of teleost fish consists of a sequence of stereotyped actions. By observing mating of zebrafish under high-speed video, we analyzed and characterized a behavioral cascade leading to successful fertilization. When paired, a male zebrafish engages the female by oscillating...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-99638-6 |
| _version_ | 1819148619928829952 |
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| author | Buntaro Zempo Natsuko Tanaka Eriko Daikoku Fumihito Ono |
| author_facet | Buntaro Zempo Natsuko Tanaka Eriko Daikoku Fumihito Ono |
| author_sort | Buntaro Zempo |
| collection | DOAJ |
| description | Abstract The mating behavior of teleost fish consists of a sequence of stereotyped actions. By observing mating of zebrafish under high-speed video, we analyzed and characterized a behavioral cascade leading to successful fertilization. When paired, a male zebrafish engages the female by oscillating his body in high frequency (quivering). In response, the female pauses swimming and bends her body (freezing). Subsequently, the male contorts his trunk to enfold the female’s trunk. This behavior is known as wrap around. Here, we found that wrap around behavior consists of two previously unidentified components. After both sexes contort their trunks, the male adjusts until his trunk compresses the female’s dorsal fin (hooking). After hooking, the male trunk slides away from the female’s dorsal fin, simultaneously sliding his pectoral fin across the female’s gravid belly, stimulating egg release (squeezing/spawning). Orchestrated coordination of spawning presumably increases fertilization success. Surgical removal of the female dorsal fin inhibited hooking and the transition to squeezing. In a neuromuscular mutant where males lack quivering, female freezing and subsequent courtship behaviors were absent. We thus identified traits of zebrafish mating behavior and clarified their roles in successful mating. |
| first_indexed | 2024-12-22T13:48:36Z |
| format | Article |
| id | doaj.art-7dfd508390ad4e01a85f4142d730d22c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T13:48:36Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7dfd508390ad4e01a85f4142d730d22c2022-12-21T18:23:43ZengNature PortfolioScientific Reports2045-23222021-10-011111910.1038/s41598-021-99638-6High-speed camera recordings uncover previously unidentified elements of zebrafish mating behaviors integral to successful fertilizationBuntaro Zempo0Natsuko Tanaka1Eriko Daikoku2Fumihito Ono3Department of Physiology, Osaka Medical and Pharmaceutical UniversityDepartment of Physiology, Osaka Medical and Pharmaceutical UniversityDepartment of Physiology, Osaka Medical and Pharmaceutical UniversityDepartment of Physiology, Osaka Medical and Pharmaceutical UniversityAbstract The mating behavior of teleost fish consists of a sequence of stereotyped actions. By observing mating of zebrafish under high-speed video, we analyzed and characterized a behavioral cascade leading to successful fertilization. When paired, a male zebrafish engages the female by oscillating his body in high frequency (quivering). In response, the female pauses swimming and bends her body (freezing). Subsequently, the male contorts his trunk to enfold the female’s trunk. This behavior is known as wrap around. Here, we found that wrap around behavior consists of two previously unidentified components. After both sexes contort their trunks, the male adjusts until his trunk compresses the female’s dorsal fin (hooking). After hooking, the male trunk slides away from the female’s dorsal fin, simultaneously sliding his pectoral fin across the female’s gravid belly, stimulating egg release (squeezing/spawning). Orchestrated coordination of spawning presumably increases fertilization success. Surgical removal of the female dorsal fin inhibited hooking and the transition to squeezing. In a neuromuscular mutant where males lack quivering, female freezing and subsequent courtship behaviors were absent. We thus identified traits of zebrafish mating behavior and clarified their roles in successful mating.https://doi.org/10.1038/s41598-021-99638-6 |
| spellingShingle | Buntaro Zempo Natsuko Tanaka Eriko Daikoku Fumihito Ono High-speed camera recordings uncover previously unidentified elements of zebrafish mating behaviors integral to successful fertilization Scientific Reports |
| title | High-speed camera recordings uncover previously unidentified elements of zebrafish mating behaviors integral to successful fertilization |
| title_full | High-speed camera recordings uncover previously unidentified elements of zebrafish mating behaviors integral to successful fertilization |
| title_fullStr | High-speed camera recordings uncover previously unidentified elements of zebrafish mating behaviors integral to successful fertilization |
| title_full_unstemmed | High-speed camera recordings uncover previously unidentified elements of zebrafish mating behaviors integral to successful fertilization |
| title_short | High-speed camera recordings uncover previously unidentified elements of zebrafish mating behaviors integral to successful fertilization |
| title_sort | high speed camera recordings uncover previously unidentified elements of zebrafish mating behaviors integral to successful fertilization |
| url | https://doi.org/10.1038/s41598-021-99638-6 |
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