Involvement of the MetO/Msr System in Two <i>Acer</i> Species That Display Contrasting Characteristics during Germination
The levels of methionine sulfoxide (MetO) and the abundances of methionine sulfoxide reductases (Msrs) were reported as important for the desiccation tolerance of <i>Acer</i> seeds. To determine whether the MetO/Msrs system is related to reactive oxygen species (ROS) and involved in the...
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/23/9197 |
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| author | Natalia Wojciechowska Shirin Alipour Ewelina Stolarska Karolina Bilska Pascal Rey Ewa M. Kalemba |
| author_facet | Natalia Wojciechowska Shirin Alipour Ewelina Stolarska Karolina Bilska Pascal Rey Ewa M. Kalemba |
| author_sort | Natalia Wojciechowska |
| collection | DOAJ |
| description | The levels of methionine sulfoxide (MetO) and the abundances of methionine sulfoxide reductases (Msrs) were reported as important for the desiccation tolerance of <i>Acer</i> seeds. To determine whether the MetO/Msrs system is related to reactive oxygen species (ROS) and involved in the regulation of germination in orthodox and recalcitrant seeds, Norway maple and sycamore were investigated. Changes in water content, MetO content, the abundance of MsrB1 and MsrB2 in relation to ROS content and the activity of reductases depending on nicotinamide adenine dinucleotides were monitored. <i>Acer</i> seeds differed in germination speed—substantially higher in sycamore—hydration dynamics, levels of hydrogen peroxide, superoxide anion radicals (O<sub>2</sub><sup>•−</sup>) and hydroxyl radicals (•OH), which exhibited peaks at different stages of germination. The MetO level dynamically changed, particularly in sycamore embryonic axes, where it was positively correlated with the levels of O<sub>2</sub><sup>•−</sup> and the abundance of MsrB1 and negatively with the levels of •OH and the abundance of MsrB2. The MsrB2 abundance increased upon sycamore germination; in contrast, it markedly decreased in Norway maple. We propose that the ROS–MetO–Msr redox system, allowing balanced Met redox homeostasis, participates in the germination process in sycamore, which is characterized by a much higher speed compared to Norway maple. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:22:25Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7e001fb031f74422a5fa3ffb1d6c36c92023-11-20T23:15:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-012123919710.3390/ijms21239197Involvement of the MetO/Msr System in Two <i>Acer</i> Species That Display Contrasting Characteristics during GerminationNatalia Wojciechowska0Shirin Alipour1Ewelina Stolarska2Karolina Bilska3Pascal Rey4Ewa M. Kalemba5Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, PolandInstitute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, PolandInstitute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, PolandInstitute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, PolandPlant Protective Proteins (PPV) Team, Centre National de la Recherche Scientifique (CNRS), Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA), Biosciences and Biotechnology Institute of Aix-Marseille (BIAM), Aix Marseille University (AMU), 13108 Saint Paul-Lez-Durance, FranceInstitute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, PolandThe levels of methionine sulfoxide (MetO) and the abundances of methionine sulfoxide reductases (Msrs) were reported as important for the desiccation tolerance of <i>Acer</i> seeds. To determine whether the MetO/Msrs system is related to reactive oxygen species (ROS) and involved in the regulation of germination in orthodox and recalcitrant seeds, Norway maple and sycamore were investigated. Changes in water content, MetO content, the abundance of MsrB1 and MsrB2 in relation to ROS content and the activity of reductases depending on nicotinamide adenine dinucleotides were monitored. <i>Acer</i> seeds differed in germination speed—substantially higher in sycamore—hydration dynamics, levels of hydrogen peroxide, superoxide anion radicals (O<sub>2</sub><sup>•−</sup>) and hydroxyl radicals (•OH), which exhibited peaks at different stages of germination. The MetO level dynamically changed, particularly in sycamore embryonic axes, where it was positively correlated with the levels of O<sub>2</sub><sup>•−</sup> and the abundance of MsrB1 and negatively with the levels of •OH and the abundance of MsrB2. The MsrB2 abundance increased upon sycamore germination; in contrast, it markedly decreased in Norway maple. We propose that the ROS–MetO–Msr redox system, allowing balanced Met redox homeostasis, participates in the germination process in sycamore, which is characterized by a much higher speed compared to Norway maple.https://www.mdpi.com/1422-0067/21/23/9197methionine oxidationnicotinamide adenine dinucleotide phosphateredox posttranslational modificationreactive oxygen speciesmethionine sulfoxide reductaseseeds |
| spellingShingle | Natalia Wojciechowska Shirin Alipour Ewelina Stolarska Karolina Bilska Pascal Rey Ewa M. Kalemba Involvement of the MetO/Msr System in Two <i>Acer</i> Species That Display Contrasting Characteristics during Germination International Journal of Molecular Sciences methionine oxidation nicotinamide adenine dinucleotide phosphate redox posttranslational modification reactive oxygen species methionine sulfoxide reductase seeds |
| title | Involvement of the MetO/Msr System in Two <i>Acer</i> Species That Display Contrasting Characteristics during Germination |
| title_full | Involvement of the MetO/Msr System in Two <i>Acer</i> Species That Display Contrasting Characteristics during Germination |
| title_fullStr | Involvement of the MetO/Msr System in Two <i>Acer</i> Species That Display Contrasting Characteristics during Germination |
| title_full_unstemmed | Involvement of the MetO/Msr System in Two <i>Acer</i> Species That Display Contrasting Characteristics during Germination |
| title_short | Involvement of the MetO/Msr System in Two <i>Acer</i> Species That Display Contrasting Characteristics during Germination |
| title_sort | involvement of the meto msr system in two i acer i species that display contrasting characteristics during germination |
| topic | methionine oxidation nicotinamide adenine dinucleotide phosphate redox posttranslational modification reactive oxygen species methionine sulfoxide reductase seeds |
| url | https://www.mdpi.com/1422-0067/21/23/9197 |
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