<i>Candida intermedia</i> CBS 141442: A Novel Glucose/Xylose Co-Fermenting Isolate for Lignocellulosic Bioethanol Production
The present study describes the isolation of the novel strain <i>Candida intermedia</i> CBS 141442 and investigates the potential of this microorganism for the conversion of lignocellulosic streams. Different <i>C. intermedia</i> clones were isolated during an adaptive labora...
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MDPI AG
2020-10-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/13/20/5363 |
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| author | Antonio D. Moreno Elia Tomás-Pejó Lisbeth Olsson Cecilia Geijer |
| author_facet | Antonio D. Moreno Elia Tomás-Pejó Lisbeth Olsson Cecilia Geijer |
| author_sort | Antonio D. Moreno |
| collection | DOAJ |
| description | The present study describes the isolation of the novel strain <i>Candida intermedia</i> CBS 141442 and investigates the potential of this microorganism for the conversion of lignocellulosic streams. Different <i>C. intermedia</i> clones were isolated during an adaptive laboratory evolution experiment under the selection pressure of lignocellulosic hydrolysate and in strong competition with industrial, xylose-fermenting <i>Saccharomyces cerevisiae</i> cells. Isolates showed different but stable colony and cell morphologies when growing in a solid agar medium (smooth, intermediate and complex morphology) and liquid medium (unicellular, aggregates and pseudohyphal morphology). Clones of the same morphology showed similar fermentation patterns, and the <i>C. intermedia</i> clone I5 (CBS 141442) was selected for further testing due to its superior capacity for xylose consumption (90% of the initial xylose concentration within 72 h) and the highest ethanol yields (0.25 ± 0.02 g ethanol/g sugars consumed). Compared to the well-known yeast <i>Scheffersomyces stipitis</i>, the selected strain showed slightly higher tolerance to the lignocellulosic-derived inhibitors when fermenting a wheat straw hydrolysate. Furthermore, its higher glucose consumption rates (compared to <i>S. stipitis</i>) and its capacity for glucose and xylose co-fermentation makes <i>C. intermedia</i> CBS 141442 an attractive microorganism for the conversion of lignocellulosic substrates, as demonstrated in simultaneous saccharification and fermentation processes. |
| first_indexed | 2024-03-10T15:37:33Z |
| format | Article |
| id | doaj.art-7e012b2ccb85427b94745a5c94e2d395 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-10T15:37:33Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-7e012b2ccb85427b94745a5c94e2d3952023-11-20T17:08:22ZengMDPI AGEnergies1996-10732020-10-011320536310.3390/en13205363<i>Candida intermedia</i> CBS 141442: A Novel Glucose/Xylose Co-Fermenting Isolate for Lignocellulosic Bioethanol ProductionAntonio D. Moreno0Elia Tomás-Pejó1Lisbeth Olsson2Cecilia Geijer3Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96 Gothenburg, SwedenDepartment of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96 Gothenburg, SwedenDepartment of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96 Gothenburg, SwedenDepartment of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96 Gothenburg, SwedenThe present study describes the isolation of the novel strain <i>Candida intermedia</i> CBS 141442 and investigates the potential of this microorganism for the conversion of lignocellulosic streams. Different <i>C. intermedia</i> clones were isolated during an adaptive laboratory evolution experiment under the selection pressure of lignocellulosic hydrolysate and in strong competition with industrial, xylose-fermenting <i>Saccharomyces cerevisiae</i> cells. Isolates showed different but stable colony and cell morphologies when growing in a solid agar medium (smooth, intermediate and complex morphology) and liquid medium (unicellular, aggregates and pseudohyphal morphology). Clones of the same morphology showed similar fermentation patterns, and the <i>C. intermedia</i> clone I5 (CBS 141442) was selected for further testing due to its superior capacity for xylose consumption (90% of the initial xylose concentration within 72 h) and the highest ethanol yields (0.25 ± 0.02 g ethanol/g sugars consumed). Compared to the well-known yeast <i>Scheffersomyces stipitis</i>, the selected strain showed slightly higher tolerance to the lignocellulosic-derived inhibitors when fermenting a wheat straw hydrolysate. Furthermore, its higher glucose consumption rates (compared to <i>S. stipitis</i>) and its capacity for glucose and xylose co-fermentation makes <i>C. intermedia</i> CBS 141442 an attractive microorganism for the conversion of lignocellulosic substrates, as demonstrated in simultaneous saccharification and fermentation processes.https://www.mdpi.com/1996-1073/13/20/5363yeast robustnessyeast morphologysimultaneous saccharification and fermentationbiofuelscell factory |
| spellingShingle | Antonio D. Moreno Elia Tomás-Pejó Lisbeth Olsson Cecilia Geijer <i>Candida intermedia</i> CBS 141442: A Novel Glucose/Xylose Co-Fermenting Isolate for Lignocellulosic Bioethanol Production Energies yeast robustness yeast morphology simultaneous saccharification and fermentation biofuels cell factory |
| title | <i>Candida intermedia</i> CBS 141442: A Novel Glucose/Xylose Co-Fermenting Isolate for Lignocellulosic Bioethanol Production |
| title_full | <i>Candida intermedia</i> CBS 141442: A Novel Glucose/Xylose Co-Fermenting Isolate for Lignocellulosic Bioethanol Production |
| title_fullStr | <i>Candida intermedia</i> CBS 141442: A Novel Glucose/Xylose Co-Fermenting Isolate for Lignocellulosic Bioethanol Production |
| title_full_unstemmed | <i>Candida intermedia</i> CBS 141442: A Novel Glucose/Xylose Co-Fermenting Isolate for Lignocellulosic Bioethanol Production |
| title_short | <i>Candida intermedia</i> CBS 141442: A Novel Glucose/Xylose Co-Fermenting Isolate for Lignocellulosic Bioethanol Production |
| title_sort | i candida intermedia i cbs 141442 a novel glucose xylose co fermenting isolate for lignocellulosic bioethanol production |
| topic | yeast robustness yeast morphology simultaneous saccharification and fermentation biofuels cell factory |
| url | https://www.mdpi.com/1996-1073/13/20/5363 |
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