Developing microsatellite duplex PCR reactions for sterlet (Acipenser ruthenus) and their application in parentage identification
Abstract The sterlet (Acipenser ruthenus) is one of the 27 sturgeon species and is well-known for its wide distribution and small body size in comparison to other sturgeons. For assessing the population genetics and parentage identification of sterlet, ten microsatellites developed for Chinese sturg...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-16194-3 |
| _version_ | 1818474080276316160 |
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| author | Jun Wang Zhiwei Sun Linlang Jiang Yacheng Hu |
| author_facet | Jun Wang Zhiwei Sun Linlang Jiang Yacheng Hu |
| author_sort | Jun Wang |
| collection | DOAJ |
| description | Abstract The sterlet (Acipenser ruthenus) is one of the 27 sturgeon species and is well-known for its wide distribution and small body size in comparison to other sturgeons. For assessing the population genetics and parentage identification of sterlet, ten microsatellites developed for Chinese sturgeon and cross-amplified in sterlet were tested by 40 individuals of sterlet. The ten microsatellites were developed using transcriptome sequencing of Chinese sturgeon. The expected heterozygosity (H E ), observed heterozygosity (H O ), Shannon-Weiner diversity indices (H′) and polymorphic information content (PIC) of the 10 microsatellites ranged from 0.466 to 0.751, from 0.438 to 0.938, from 0.66 to 1.51 and from 0.368 to 0.716, respectively. Combined exclusion probability based on the genotype of pair parent known (CE-PP), one parent known (CE-2P), and no parent known (CE-1P) of the 10 microsatellites were 99.99%, 99.96%, and 99.49%, respectively. These result showed that the 10 microsatellites should be helpful for assessing the population genetics and parentage identification of sterlet. |
| first_indexed | 2024-04-14T04:31:45Z |
| format | Article |
| id | doaj.art-7e024f5b552a4bf7a4360f9d0eefca13 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-14T04:31:45Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7e024f5b552a4bf7a4360f9d0eefca132022-12-22T02:12:01ZengNature PortfolioScientific Reports2045-23222022-07-011211710.1038/s41598-022-16194-3Developing microsatellite duplex PCR reactions for sterlet (Acipenser ruthenus) and their application in parentage identificationJun Wang0Zhiwei Sun1Linlang Jiang2Yacheng Hu3State Key Laboratory of Eco-Hydraulics in Northwest Arid Region of China, Xi’an University of TechnologyState Key Laboratory of Eco-Hydraulics in Northwest Arid Region of China, Xi’an University of TechnologyState Key Laboratory of Eco-Hydraulics in Northwest Arid Region of China, Xi’an University of TechnologyChinese Sturgeon Research Institute, China Three Gorges CorporationAbstract The sterlet (Acipenser ruthenus) is one of the 27 sturgeon species and is well-known for its wide distribution and small body size in comparison to other sturgeons. For assessing the population genetics and parentage identification of sterlet, ten microsatellites developed for Chinese sturgeon and cross-amplified in sterlet were tested by 40 individuals of sterlet. The ten microsatellites were developed using transcriptome sequencing of Chinese sturgeon. The expected heterozygosity (H E ), observed heterozygosity (H O ), Shannon-Weiner diversity indices (H′) and polymorphic information content (PIC) of the 10 microsatellites ranged from 0.466 to 0.751, from 0.438 to 0.938, from 0.66 to 1.51 and from 0.368 to 0.716, respectively. Combined exclusion probability based on the genotype of pair parent known (CE-PP), one parent known (CE-2P), and no parent known (CE-1P) of the 10 microsatellites were 99.99%, 99.96%, and 99.49%, respectively. These result showed that the 10 microsatellites should be helpful for assessing the population genetics and parentage identification of sterlet.https://doi.org/10.1038/s41598-022-16194-3 |
| spellingShingle | Jun Wang Zhiwei Sun Linlang Jiang Yacheng Hu Developing microsatellite duplex PCR reactions for sterlet (Acipenser ruthenus) and their application in parentage identification Scientific Reports |
| title | Developing microsatellite duplex PCR reactions for sterlet (Acipenser ruthenus) and their application in parentage identification |
| title_full | Developing microsatellite duplex PCR reactions for sterlet (Acipenser ruthenus) and their application in parentage identification |
| title_fullStr | Developing microsatellite duplex PCR reactions for sterlet (Acipenser ruthenus) and their application in parentage identification |
| title_full_unstemmed | Developing microsatellite duplex PCR reactions for sterlet (Acipenser ruthenus) and their application in parentage identification |
| title_short | Developing microsatellite duplex PCR reactions for sterlet (Acipenser ruthenus) and their application in parentage identification |
| title_sort | developing microsatellite duplex pcr reactions for sterlet acipenser ruthenus and their application in parentage identification |
| url | https://doi.org/10.1038/s41598-022-16194-3 |
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