Nuclear Akt promotes neurite outgrowth in the early stage of neuritogenesis
In addition to its pivotal role in neuronal survival, PI3K/Aktsignaling is integral to neuronal differentiation and neuriteoutgrowth. However, the exact role of Akt in neuronaldifferentiation is still controversial. Here, we found thatnuclear expression of CA-Akt resulted in unusual rapid neuriteout...
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| Format: | Article |
| Language: | English |
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Korean Society for Biochemistry and Molecular Biology
2012-09-01
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| Series: | BMB Reports |
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| Online Access: | http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS05JTVEMTIwOTI3MTUzMl8lMjg1MjEtNTI1JTI5Qk1CXzEyLTExNC5wZGY= |
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| author | Ji Hye Park1,2,3#, Sang Bae Lee1,#, Kyung-Hoon Lee2,3,* & Jee-Yin Ahn1,3,* |
| author_facet | Ji Hye Park1,2,3#, Sang Bae Lee1,#, Kyung-Hoon Lee2,3,* & Jee-Yin Ahn1,3,* |
| author_sort | Ji Hye Park1,2,3#, Sang Bae Lee1,#, Kyung-Hoon Lee2,3,* & Jee-Yin Ahn1,3,* |
| collection | DOAJ |
| description | In addition to its pivotal role in neuronal survival, PI3K/Aktsignaling is integral to neuronal differentiation and neuriteoutgrowth. However, the exact role of Akt in neuronaldifferentiation is still controversial. Here, we found thatnuclear expression of CA-Akt resulted in unusual rapid neuriteoutgrowth and overexpression of KD-Akt caused multipledendrite growth without specific axon elongation. Moreover,microarray data revealed that the expression of FOXQ1expression was about 10-fold higher in cells with nuclear,active Akt than in control cells. Quantitative real-time PCRanalysis showed that mRNA levels were upregulated inNLS-CA-Akt cells as compared to KD or EV cells. Furthermore,our FACS analysis demonstrated that overexpression ofNLS-CA-Akt accumulate cells in the G1 phase within 24 h,fitting with the rapid sprouting of neuritis. Thus, our dataimplied that at least in this early time frame, the overexpressionof nuclear, active Akt forced cells into neurite developmentthrough probably FOXQ1regulation. |
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| format | Article |
| id | doaj.art-7e03351e8a1d4d36b2c84f5308d174c3 |
| institution | Directory Open Access Journal |
| issn | 1976-6696 1976-670X |
| language | English |
| last_indexed | 2024-12-21T07:30:21Z |
| publishDate | 2012-09-01 |
| publisher | Korean Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | BMB Reports |
| spelling | doaj.art-7e03351e8a1d4d36b2c84f5308d174c32022-12-21T19:11:35ZengKorean Society for Biochemistry and Molecular BiologyBMB Reports1976-66961976-670X2012-09-01459521525Nuclear Akt promotes neurite outgrowth in the early stage of neuritogenesisJi Hye Park1,2,3#, Sang Bae Lee1,#, Kyung-Hoon Lee2,3,* & Jee-Yin Ahn1,3,*In addition to its pivotal role in neuronal survival, PI3K/Aktsignaling is integral to neuronal differentiation and neuriteoutgrowth. However, the exact role of Akt in neuronaldifferentiation is still controversial. Here, we found thatnuclear expression of CA-Akt resulted in unusual rapid neuriteoutgrowth and overexpression of KD-Akt caused multipledendrite growth without specific axon elongation. Moreover,microarray data revealed that the expression of FOXQ1expression was about 10-fold higher in cells with nuclear,active Akt than in control cells. Quantitative real-time PCRanalysis showed that mRNA levels were upregulated inNLS-CA-Akt cells as compared to KD or EV cells. Furthermore,our FACS analysis demonstrated that overexpression ofNLS-CA-Akt accumulate cells in the G1 phase within 24 h,fitting with the rapid sprouting of neuritis. Thus, our dataimplied that at least in this early time frame, the overexpressionof nuclear, active Akt forced cells into neurite developmentthrough probably FOXQ1regulation.http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS05JTVEMTIwOTI3MTUzMl8lMjg1MjEtNTI1JTI5Qk1CXzEyLTExNC5wZGY=AktFOXQ1Neurite outgrowth |
| spellingShingle | Ji Hye Park1,2,3#, Sang Bae Lee1,#, Kyung-Hoon Lee2,3,* & Jee-Yin Ahn1,3,* Nuclear Akt promotes neurite outgrowth in the early stage of neuritogenesis BMB Reports Akt FOXQ1 Neurite outgrowth |
| title | Nuclear Akt promotes neurite outgrowth in the early stage of neuritogenesis |
| title_full | Nuclear Akt promotes neurite outgrowth in the early stage of neuritogenesis |
| title_fullStr | Nuclear Akt promotes neurite outgrowth in the early stage of neuritogenesis |
| title_full_unstemmed | Nuclear Akt promotes neurite outgrowth in the early stage of neuritogenesis |
| title_short | Nuclear Akt promotes neurite outgrowth in the early stage of neuritogenesis |
| title_sort | nuclear akt promotes neurite outgrowth in the early stage of neuritogenesis |
| topic | Akt FOXQ1 Neurite outgrowth |
| url | http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS05JTVEMTIwOTI3MTUzMl8lMjg1MjEtNTI1JTI5Qk1CXzEyLTExNC5wZGY= |
| work_keys_str_mv | AT jihyepark123sangbaelee1kyunghoonlee23jeeyinahn13 nuclearaktpromotesneuriteoutgrowthintheearlystageofneuritogenesis |