Protocol to study starvation-induced autophagy in developing rat neurons
Summary: Autophagy is being involved in an increasing number of cellular pathways. It now appears that autophagy stimulation and inhibition have complex effects in neurons. Here, we present a simple yet powerful protocol to induce autophagy in primary neurons in culture by partial nutrient deprivati...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721004202 |
| _version_ | 1818717505651212288 |
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| author | José Wojnacki Sébastien Nola Thierry Galli |
| author_facet | José Wojnacki Sébastien Nola Thierry Galli |
| author_sort | José Wojnacki |
| collection | DOAJ |
| description | Summary: Autophagy is being involved in an increasing number of cellular pathways. It now appears that autophagy stimulation and inhibition have complex effects in neurons. Here, we present a simple yet powerful protocol to induce autophagy in primary neurons in culture by partial nutrient deprivation, in neurons with or without transfection of plasmids encoding the Longin domain of VAMP7 or a nanobody directed against VAMP7. Although limited to cells in culture, this protocol can facilitate the study of autophagy in neurons.For complete details on the use and execution of this protocol, please refer to Wojnacki et al. (2020). |
| first_indexed | 2024-12-17T19:36:13Z |
| format | Article |
| id | doaj.art-7e035b0e786e46009c9c38ba0481d231 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T19:36:13Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-7e035b0e786e46009c9c38ba0481d2312022-12-21T21:35:09ZengElsevierSTAR Protocols2666-16672021-09-0123100713Protocol to study starvation-induced autophagy in developing rat neuronsJosé Wojnacki0Sébastien Nola1Thierry Galli2Université de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, 75014 Paris, France; Corresponding authorUniversité de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, 75014 Paris, FranceUniversité de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, 75014 Paris, France; GHU PARIS psychiatrie & neurosciences, 75014 Paris, France; Corresponding authorSummary: Autophagy is being involved in an increasing number of cellular pathways. It now appears that autophagy stimulation and inhibition have complex effects in neurons. Here, we present a simple yet powerful protocol to induce autophagy in primary neurons in culture by partial nutrient deprivation, in neurons with or without transfection of plasmids encoding the Longin domain of VAMP7 or a nanobody directed against VAMP7. Although limited to cells in culture, this protocol can facilitate the study of autophagy in neurons.For complete details on the use and execution of this protocol, please refer to Wojnacki et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721004202AntibodyCell BiologyCell cultureMicroscopyNeuroscience |
| spellingShingle | José Wojnacki Sébastien Nola Thierry Galli Protocol to study starvation-induced autophagy in developing rat neurons STAR Protocols Antibody Cell Biology Cell culture Microscopy Neuroscience |
| title | Protocol to study starvation-induced autophagy in developing rat neurons |
| title_full | Protocol to study starvation-induced autophagy in developing rat neurons |
| title_fullStr | Protocol to study starvation-induced autophagy in developing rat neurons |
| title_full_unstemmed | Protocol to study starvation-induced autophagy in developing rat neurons |
| title_short | Protocol to study starvation-induced autophagy in developing rat neurons |
| title_sort | protocol to study starvation induced autophagy in developing rat neurons |
| topic | Antibody Cell Biology Cell culture Microscopy Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721004202 |
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