Metabolic Profiling of Cells in Response to Drug Treatment using 1H High-resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy
High-resolution magic angle spinning (HR-MAS) is an NMR technique that provides access to well resolved liquid-like 1H NMR spectra of semi-solid samples. Therefore, 1H HR-MAS NMR spectroscopy has become an important tool for the direct analysis of biological samples such as tissues and cel...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2017-03-01
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| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://www.chimia.ch/chimia/article/view/1570 |
| _version_ | 1818295796599095296 |
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| author | Martina Vermathen Gaëlle Diserens Peter Vermathen Julien Furrer |
| author_facet | Martina Vermathen Gaëlle Diserens Peter Vermathen Julien Furrer |
| author_sort | Martina Vermathen |
| collection | DOAJ |
| description |
High-resolution magic angle spinning (HR-MAS) is an NMR technique that provides access to well resolved liquid-like 1H NMR spectra of semi-solid samples. Therefore, 1H HR-MAS NMR spectroscopy has become an important tool for the direct analysis of biological samples
such as tissues and cells in a mostly non-destructive way. Here, we focus on the application of HR-MAS NMR combined with multivariate statistical methods used for metabolic profiling of cells and in particular for the study of cellular metabolic responses to drug exposure. The principles of
HR-MAS and the metabolomic approach are briefly described. As an example, a study on the metabolic response of different cell types towards treatment with a highly cytotoxic hexacationic ruthenium metallaprism as potential anti-cancer drug is presented. Specific metabolites and metabolic pathways
are suggested to be associated with the cellular response. The study demonstrates the potential of HR-MAS metabolomics applied to cells for addressing the intracellular processes involved in the treatment with organometallic drugs.
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| first_indexed | 2024-12-13T03:53:20Z |
| format | Article |
| id | doaj.art-7e03d6d258ea47ed991e9ba4dc06ebc0 |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-12-13T03:53:20Z |
| publishDate | 2017-03-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-7e03d6d258ea47ed991e9ba4dc06ebc02022-12-22T00:00:40ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242017-03-0171310.2533/chimia.2017.124Metabolic Profiling of Cells in Response to Drug Treatment using 1H High-resolution Magic Angle Spinning (HR-MAS) NMR SpectroscopyMartina Vermathen0Gaëlle Diserens1Peter Vermathen2Julien Furrer3Department of Chemistry and Biochemistry University of Bern, Freiestrasse 3 CH-3012 Bern, Switzerland. martina.vermathen@dcb.unibe.chDepartments of Clinical Research and Radiology, University and Inselspital Bern, SwitzerlandDepartments of Clinical Research and Radiology, University and Inselspital Bern, SwitzerlandDepartment of Chemistry and Biochemistry University of Bern, Freiestrasse 3 CH-3012 Bern, Switzerland;, Email: julien.furrer@dcb.unibe.ch High-resolution magic angle spinning (HR-MAS) is an NMR technique that provides access to well resolved liquid-like 1H NMR spectra of semi-solid samples. Therefore, 1H HR-MAS NMR spectroscopy has become an important tool for the direct analysis of biological samples such as tissues and cells in a mostly non-destructive way. Here, we focus on the application of HR-MAS NMR combined with multivariate statistical methods used for metabolic profiling of cells and in particular for the study of cellular metabolic responses to drug exposure. The principles of HR-MAS and the metabolomic approach are briefly described. As an example, a study on the metabolic response of different cell types towards treatment with a highly cytotoxic hexacationic ruthenium metallaprism as potential anti-cancer drug is presented. Specific metabolites and metabolic pathways are suggested to be associated with the cellular response. The study demonstrates the potential of HR-MAS metabolomics applied to cells for addressing the intracellular processes involved in the treatment with organometallic drugs. https://www.chimia.ch/chimia/article/view/1570CellsHr mas nmrMetabolic profilingRuthenium |
| spellingShingle | Martina Vermathen Gaëlle Diserens Peter Vermathen Julien Furrer Metabolic Profiling of Cells in Response to Drug Treatment using 1H High-resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy CHIMIA Cells Hr mas nmr Metabolic profiling Ruthenium |
| title | Metabolic Profiling of Cells in Response to Drug Treatment using 1H High-resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy |
| title_full | Metabolic Profiling of Cells in Response to Drug Treatment using 1H High-resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy |
| title_fullStr | Metabolic Profiling of Cells in Response to Drug Treatment using 1H High-resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy |
| title_full_unstemmed | Metabolic Profiling of Cells in Response to Drug Treatment using 1H High-resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy |
| title_short | Metabolic Profiling of Cells in Response to Drug Treatment using 1H High-resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy |
| title_sort | metabolic profiling of cells in response to drug treatment using 1h high resolution magic angle spinning hr mas nmr spectroscopy |
| topic | Cells Hr mas nmr Metabolic profiling Ruthenium |
| url | https://www.chimia.ch/chimia/article/view/1570 |
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