Production of Salvianic Acid A from <span style="font-variant: small-caps">l</span>-DOPA via Biocatalytic Cascade Reactions
Salvianic acid A (SAA), as the main bioactive component of the traditional Chinese herb <i>Salvia miltiorrhiza</i>, has important application value in the treatment of cardiovascular diseases. In this study, a two-step bioprocess for the preparation of SAA from <span style="font-...
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/18/6088 |
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| author | Ke Shun Hu Chong Le Chen Huan Ru Ding Tian Yu Wang Qin Zhu Yi Chen Zhou Jia Min Chen Jia Qi Mei Sheng Hu Jun Huang Wei Rui Zhao Le He Mei |
| author_facet | Ke Shun Hu Chong Le Chen Huan Ru Ding Tian Yu Wang Qin Zhu Yi Chen Zhou Jia Min Chen Jia Qi Mei Sheng Hu Jun Huang Wei Rui Zhao Le He Mei |
| author_sort | Ke Shun Hu |
| collection | DOAJ |
| description | Salvianic acid A (SAA), as the main bioactive component of the traditional Chinese herb <i>Salvia miltiorrhiza</i>, has important application value in the treatment of cardiovascular diseases. In this study, a two-step bioprocess for the preparation of SAA from <span style="font-variant: small-caps;">l</span>-DOPA was developed. In the first step, <span style="font-variant: small-caps;">l</span>-DOPA was transformed to 3,4-dihydroxyphenylalanine (DHPPA) using engineered <i>Escherichia coli</i> cells expressing membrane-bound L-amino acid deaminase from <i>Proteus vulgaris.</i> After that, the unpurified DHPPA was directly converted into SAA by permeabilized recombinant <i>E. coli</i> cells co-expressing <span style="font-variant: small-caps;">d</span>-lactate dehydrogenase from <i>Pediococcus acidilactici</i> and formate dehydrogenase from <i>Mycobacterium vaccae</i> N10. Under optimized conditions, 48.3 mM of SAA could be prepared from 50 mM of <span style="font-variant: small-caps;">l</span>-DOPA, with a yield of 96.6%. Therefore, the bioprocess developed here was not only environmentally friendly, but also exhibited excellent production efficiency and, thus, is promising for industrial SAA production. |
| first_indexed | 2024-03-09T23:00:08Z |
| format | Article |
| id | doaj.art-7e0b02c16a3d40b79f500f2d257c3373 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:00:08Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7e0b02c16a3d40b79f500f2d257c33732023-11-23T18:04:12ZengMDPI AGMolecules1420-30492022-09-012718608810.3390/molecules27186088Production of Salvianic Acid A from <span style="font-variant: small-caps">l</span>-DOPA via Biocatalytic Cascade ReactionsKe Shun Hu0Chong Le Chen1Huan Ru Ding2Tian Yu Wang3Qin Zhu4Yi Chen Zhou5Jia Min Chen6Jia Qi Mei7Sheng Hu8Jun Huang9Wei Rui Zhao10Le He Mei11School of Biotechnology and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biotechnology and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biotechnology and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biotechnology and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biotechnology and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biotechnology and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biotechnology and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaHangzhou Huadong Medicine Group Co. Ltd., Hangzhou 310011, ChinaSchool of Biotechnology and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaDepartment of Chemical and Biological Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaSchool of Biotechnology and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSchool of Biotechnology and Chemical Engineering, NingboTech University, Ningbo 315100, ChinaSalvianic acid A (SAA), as the main bioactive component of the traditional Chinese herb <i>Salvia miltiorrhiza</i>, has important application value in the treatment of cardiovascular diseases. In this study, a two-step bioprocess for the preparation of SAA from <span style="font-variant: small-caps;">l</span>-DOPA was developed. In the first step, <span style="font-variant: small-caps;">l</span>-DOPA was transformed to 3,4-dihydroxyphenylalanine (DHPPA) using engineered <i>Escherichia coli</i> cells expressing membrane-bound L-amino acid deaminase from <i>Proteus vulgaris.</i> After that, the unpurified DHPPA was directly converted into SAA by permeabilized recombinant <i>E. coli</i> cells co-expressing <span style="font-variant: small-caps;">d</span>-lactate dehydrogenase from <i>Pediococcus acidilactici</i> and formate dehydrogenase from <i>Mycobacterium vaccae</i> N10. Under optimized conditions, 48.3 mM of SAA could be prepared from 50 mM of <span style="font-variant: small-caps;">l</span>-DOPA, with a yield of 96.6%. Therefore, the bioprocess developed here was not only environmentally friendly, but also exhibited excellent production efficiency and, thus, is promising for industrial SAA production.https://www.mdpi.com/1420-3049/27/18/6088salvianic acid A<span style="font-variant: small-caps">l</span>-DOPAmembrane-bound <span style="font-variant: small-caps">l</span>-amino acid deaminasesbiocatalysismolecular biologybiological engineering |
| spellingShingle | Ke Shun Hu Chong Le Chen Huan Ru Ding Tian Yu Wang Qin Zhu Yi Chen Zhou Jia Min Chen Jia Qi Mei Sheng Hu Jun Huang Wei Rui Zhao Le He Mei Production of Salvianic Acid A from <span style="font-variant: small-caps">l</span>-DOPA via Biocatalytic Cascade Reactions Molecules salvianic acid A <span style="font-variant: small-caps">l</span>-DOPA membrane-bound <span style="font-variant: small-caps">l</span>-amino acid deaminases biocatalysis molecular biology biological engineering |
| title | Production of Salvianic Acid A from <span style="font-variant: small-caps">l</span>-DOPA via Biocatalytic Cascade Reactions |
| title_full | Production of Salvianic Acid A from <span style="font-variant: small-caps">l</span>-DOPA via Biocatalytic Cascade Reactions |
| title_fullStr | Production of Salvianic Acid A from <span style="font-variant: small-caps">l</span>-DOPA via Biocatalytic Cascade Reactions |
| title_full_unstemmed | Production of Salvianic Acid A from <span style="font-variant: small-caps">l</span>-DOPA via Biocatalytic Cascade Reactions |
| title_short | Production of Salvianic Acid A from <span style="font-variant: small-caps">l</span>-DOPA via Biocatalytic Cascade Reactions |
| title_sort | production of salvianic acid a from span style font variant small caps l span dopa via biocatalytic cascade reactions |
| topic | salvianic acid A <span style="font-variant: small-caps">l</span>-DOPA membrane-bound <span style="font-variant: small-caps">l</span>-amino acid deaminases biocatalysis molecular biology biological engineering |
| url | https://www.mdpi.com/1420-3049/27/18/6088 |
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