Study on the mechanisms of compound Kushen injection for the treatment of gastric cancer based on network pharmacology
Abstract Background As an effective prescription for gastric cancer (GC), Compound Kushen Injection (CKI) has been widely used even though few molecular mechanism analyses have been carried out. Methods In this study, we identified 16 active ingredients and 60 GC target proteins. Then, we establishe...
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-019-2787-y |
| _version_ | 1819297330705203200 |
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| author | Wei Zhou Jiarui Wu Yingli Zhu Ziqi Meng Xinkui Liu Shuyu Liu Mengwei Ni Shanshan Jia Jingyuan Zhang Siyu Guo |
| author_facet | Wei Zhou Jiarui Wu Yingli Zhu Ziqi Meng Xinkui Liu Shuyu Liu Mengwei Ni Shanshan Jia Jingyuan Zhang Siyu Guo |
| author_sort | Wei Zhou |
| collection | DOAJ |
| description | Abstract Background As an effective prescription for gastric cancer (GC), Compound Kushen Injection (CKI) has been widely used even though few molecular mechanism analyses have been carried out. Methods In this study, we identified 16 active ingredients and 60 GC target proteins. Then, we established a compound-predicted target network and a GC target protein-protein interaction (PPI) network by Cytoscape 3.5.1 and systematically analyzed the potential targets of CKI for the treatment of GC. Finally, molecular docking was applied to verify the key targets. In addition, we analyzed the mechanism of action of the predicted targets by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses. Results The results showed that the potential targets, including CCND1, PIK3CA, AKT1, MAPK1, ERBB2, and MMP2, are the therapeutic targets of CKI for the treatment of GC. Functional enrichment analysis indicated that CKI has a therapeutic effect on GC by synergistically regulating some biological pathways, such as the cell cycle, pathways in cancer, the PI3K-AKT signaling pathway, the mTOR signaling pathway, and the FoxO signaling pathway. Moreover, molecular docking simulation indicated that the compounds had good binding activity to PIK3CA, AKT1, MAPK1, ERBB2, and MMP2 in vivo. Conclusion This research partially highlighted the molecular mechanism of CKI for the treatment of GC, which has great potential in the identification of the effective compounds in CKI and biomarkers to treat GC. |
| first_indexed | 2024-12-24T05:12:18Z |
| format | Article |
| id | doaj.art-7e0b08be245640b2bf08c22258f81f94 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-24T05:12:18Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-7e0b08be245640b2bf08c22258f81f942022-12-21T17:13:40ZengBMCBMC Complementary Medicine and Therapies2662-76712020-01-0120111510.1186/s12906-019-2787-yStudy on the mechanisms of compound Kushen injection for the treatment of gastric cancer based on network pharmacologyWei Zhou0Jiarui Wu1Yingli Zhu2Ziqi Meng3Xinkui Liu4Shuyu Liu5Mengwei Ni6Shanshan Jia7Jingyuan Zhang8Siyu Guo9Department of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese MedicineDepartment of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese MedicineDepartment of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese MedicineDepartment of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese MedicineDepartment of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese MedicineDepartment of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese MedicineDepartment of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese MedicineDepartment of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese MedicineDepartment of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese MedicineDepartment of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese MedicineAbstract Background As an effective prescription for gastric cancer (GC), Compound Kushen Injection (CKI) has been widely used even though few molecular mechanism analyses have been carried out. Methods In this study, we identified 16 active ingredients and 60 GC target proteins. Then, we established a compound-predicted target network and a GC target protein-protein interaction (PPI) network by Cytoscape 3.5.1 and systematically analyzed the potential targets of CKI for the treatment of GC. Finally, molecular docking was applied to verify the key targets. In addition, we analyzed the mechanism of action of the predicted targets by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses. Results The results showed that the potential targets, including CCND1, PIK3CA, AKT1, MAPK1, ERBB2, and MMP2, are the therapeutic targets of CKI for the treatment of GC. Functional enrichment analysis indicated that CKI has a therapeutic effect on GC by synergistically regulating some biological pathways, such as the cell cycle, pathways in cancer, the PI3K-AKT signaling pathway, the mTOR signaling pathway, and the FoxO signaling pathway. Moreover, molecular docking simulation indicated that the compounds had good binding activity to PIK3CA, AKT1, MAPK1, ERBB2, and MMP2 in vivo. Conclusion This research partially highlighted the molecular mechanism of CKI for the treatment of GC, which has great potential in the identification of the effective compounds in CKI and biomarkers to treat GC.https://doi.org/10.1186/s12906-019-2787-yCompound Kushen injectionGastric CancerNetwork pharmacology |
| spellingShingle | Wei Zhou Jiarui Wu Yingli Zhu Ziqi Meng Xinkui Liu Shuyu Liu Mengwei Ni Shanshan Jia Jingyuan Zhang Siyu Guo Study on the mechanisms of compound Kushen injection for the treatment of gastric cancer based on network pharmacology BMC Complementary Medicine and Therapies Compound Kushen injection Gastric Cancer Network pharmacology |
| title | Study on the mechanisms of compound Kushen injection for the treatment of gastric cancer based on network pharmacology |
| title_full | Study on the mechanisms of compound Kushen injection for the treatment of gastric cancer based on network pharmacology |
| title_fullStr | Study on the mechanisms of compound Kushen injection for the treatment of gastric cancer based on network pharmacology |
| title_full_unstemmed | Study on the mechanisms of compound Kushen injection for the treatment of gastric cancer based on network pharmacology |
| title_short | Study on the mechanisms of compound Kushen injection for the treatment of gastric cancer based on network pharmacology |
| title_sort | study on the mechanisms of compound kushen injection for the treatment of gastric cancer based on network pharmacology |
| topic | Compound Kushen injection Gastric Cancer Network pharmacology |
| url | https://doi.org/10.1186/s12906-019-2787-y |
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