Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size
Gonadotropin-inhibitory hormone (GnIH) can decrease luteinizing hormone and/or follicle-stimulating hormone levels in rat, mouse, sheep, and cattle by the direct suppression of gonadotropin-releasing hormone (GnRH). The present study investigated polymorphisms in the GnIH genes of two dairy goat bre...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2017-07-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201707-0001_two-single-nucleotide-polymorphisms-in-the-caprine-gnih-gene-are-associated-with-litter-size.php |
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| author | Xiaopeng An Lijuan Bao Jinxing Hou Yueyu Bai Xinyan Zhao Yuxuan Song Binyun Cao |
| author_facet | Xiaopeng An Lijuan Bao Jinxing Hou Yueyu Bai Xinyan Zhao Yuxuan Song Binyun Cao |
| author_sort | Xiaopeng An |
| collection | DOAJ |
| description | Gonadotropin-inhibitory hormone (GnIH) can decrease luteinizing hormone and/or follicle-stimulating hormone levels in rat, mouse, sheep, and cattle by the direct suppression of gonadotropin-releasing hormone (GnRH). The present study investigated polymorphisms in the GnIH genes of two dairy goat breeds and their association with litter size. Single nucleotide polymorphisms (SNPs) g.1837C>G and g.3195G>A (GenBank Accession Nos. KR778885 and KR819142) were detected in the GnIH genes of Xinong Saanen and Guanzhong dairy goat breeds using DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Furthermore, the g.1837C>G and g.3195G>A loci were closely linked in both breeds (r2 > 0.33). Association analysis showed that these SNPs had significant effects on the litter size of goats (P < 0.05). In both breeds, individuals with the CC/GG (g.1837C>G/g.3195G>A) genotype showed larger litter sizes in the second and average parities than individuals with the GG/AA genotype (P < 0.05). Known biochemical and physiological functions, along with our results, indicate that the CC/GG genotype may be used in marker-assisted selection to choose individuals with a larger litter size from both breeds. |
| first_indexed | 2024-04-10T08:25:09Z |
| format | Article |
| id | doaj.art-7e0b5960580f431e81052b51ace9619e |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:09Z |
| publishDate | 2017-07-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-7e0b5960580f431e81052b51ace9619e2023-02-23T03:33:30ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092017-07-0162726927510.17221/58/2016-CJAScjs-201707-0001Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter sizeXiaopeng An0Lijuan Bao1Jinxing Hou2Yueyu Bai3Xinyan Zhao4Yuxuan Song5Binyun Cao6College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaAnimal Health Supervision Institute of Henan Province, Zhengzhou, P.R. ChinaNorthwest A&F University of Hospital, Northwest A&F University, Yangling, P.R. ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, P.R. ChinaGonadotropin-inhibitory hormone (GnIH) can decrease luteinizing hormone and/or follicle-stimulating hormone levels in rat, mouse, sheep, and cattle by the direct suppression of gonadotropin-releasing hormone (GnRH). The present study investigated polymorphisms in the GnIH genes of two dairy goat breeds and their association with litter size. Single nucleotide polymorphisms (SNPs) g.1837C>G and g.3195G>A (GenBank Accession Nos. KR778885 and KR819142) were detected in the GnIH genes of Xinong Saanen and Guanzhong dairy goat breeds using DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Furthermore, the g.1837C>G and g.3195G>A loci were closely linked in both breeds (r2 > 0.33). Association analysis showed that these SNPs had significant effects on the litter size of goats (P < 0.05). In both breeds, individuals with the CC/GG (g.1837C>G/g.3195G>A) genotype showed larger litter sizes in the second and average parities than individuals with the GG/AA genotype (P < 0.05). Known biochemical and physiological functions, along with our results, indicate that the CC/GG genotype may be used in marker-assisted selection to choose individuals with a larger litter size from both breeds.https://cjas.agriculturejournals.cz/artkey/cjs-201707-0001_two-single-nucleotide-polymorphisms-in-the-caprine-gnih-gene-are-associated-with-litter-size.phppcr-rflpsnpscombined genotypecandidate gene |
| spellingShingle | Xiaopeng An Lijuan Bao Jinxing Hou Yueyu Bai Xinyan Zhao Yuxuan Song Binyun Cao Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size Czech Journal of Animal Science pcr-rflp snps combined genotype candidate gene |
| title | Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size |
| title_full | Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size |
| title_fullStr | Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size |
| title_full_unstemmed | Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size |
| title_short | Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size |
| title_sort | two single nucleotide polymorphisms in the caprine gnih gene are associated with litter size |
| topic | pcr-rflp snps combined genotype candidate gene |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201707-0001_two-single-nucleotide-polymorphisms-in-the-caprine-gnih-gene-are-associated-with-litter-size.php |
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